1. Plant regeneration through somatic embryogenesis and polyamine levels in cultures of grasses of Thar Desert.
- Author
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Kackar, Anup and Shekhawat, Narpat S.
- Subjects
- *
PLANT genetics , *SOMATIC embryogenesis , *POLYAMINES , *CELL culture , *GRASSES - Abstract
Tissue culture methods for plant regeneration have been developed for Lasiurus scindicus, Sorghum halepense and Urochloa panicoides using basal portion of young inflorescence as new and a potential source(s) of explants. Highly embryogenic cultures were initiated on Murashige and Skoog's (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2-4,D). The regenerative cultures were maintained by subculture on MS medium in the dark at 26±2°C. Plants were regenerated via somatic embryogenesis from the competent cell cultures on hormone-free MS basal medium or MS medium with 1 mgl-1 kinetin. Somatic seedlings were successfully transplanted to pots and grown to maturity in field. Polyamine levels were analyzed in embryogenic and non-embryogenic cultures. A correlation has been found between the individual polyamine levels and the ratio of putrescine to spermidine in the cultured tissues with plant regeneration ability via somatic embryogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2007