1. CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants.
- Author
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Osmani Z, Jin S, Mikami M, Endo M, Atarashi H, Fujino K, Yamada T, and Nakahara KS
- Subjects
- DNA Mutational Analysis, Gene Targeting, Genetic Vectors genetics, Mutagenesis, Phenotype, Transformation, Genetic, CRISPR-Cas Systems, Gene Editing, Plant Proteins genetics, Plants, Genetically Modified, Solanum tuberosum genetics
- Abstract
A tobacco calmodulin-like protein, rgs-CaM, has been shown to interact with viruses in a variety of ways; it contributes to geminivirus infections but is also involved in primed immunity to the cucumber mosaic virus. Sequence similarity searches revealed several calmodulin-like proteins similar to rgs-CaM (rCML) in Arabidopsis and other Solanaceae plants, including potato (Solanum tuberosum). To analyze the functions of each rCML, mutations were introduced into potato rCMLs using the CRISPR/Cas9 system. Here, we describe our protocol of the CRISPR/Cas9-mediated targeted mutagenesis in stably transformed potato plants.
- Published
- 2019
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