1. Effect of indole alkaloids from roots of Rauvolfia ligustrina in the noradrenergic neurotransmission.
- Author
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Magalhães HS, da Silva AB, Nascimento NRF, de Sousa LGF, da Fonseca MJS, Loiola MIB, Monteiro NKV, Almeida Neto FWQ, Canuto KM, and Pessoa ODL
- Subjects
- Animals, Brazil, Electric Stimulation, In Vitro Techniques, Indole Alkaloids chemistry, Magnetic Resonance Spectroscopy, Male, Mice, Molecular Structure, Phytochemicals isolation & purification, Phytochemicals pharmacology, Plant Extracts chemistry, Vas Deferens drug effects, Indole Alkaloids pharmacology, Plant Roots chemistry, Rauwolfia chemistry, Synaptic Transmission drug effects
- Abstract
The new glucosyl sarpagan alkaloid designated as 21(R*)-(O-β-glucosyl)-hydroxy-sarpagan-17-oic acid, along with eleven known alkaloids were isolated from a soluble alkaloidal fraction from the ethanol extract of Rauvolfia ligustrina. Their structures were elucidated by interpretation of spectroscopic data (1D and 2D NMR), HRESIMS experiment, GIAO
13 C NMR calculations, and comparison with literature data. All the isolated alkaloids were screened by their neuroinhibitory effects using the electrically stimulated mice vas deferens bioassay. Compounds 1, 2 and 9 presented a potent inhibitory effect in the neurotransmission while 3 and 11 showed an acute neuroexcitatory effect. Compound 10 exhibited a very effective post-synaptic inhibitory activity., Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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