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Organic fertilizers were applied in four different forms: mixed organic matter, fermentation cake, bacterial culture, and rice husks, excluding conventional chemical fertilizer and non-treatment used as control. The results showed that soil pH was acidified after fermentation cake and chemical fertilizer treatment. Specifically, chemical fertilizer treatment showed higher phosphoric-acid content than other treatments, while total N content was higher in fermentation cake, mixed organic matter and chemical fertilizer. Growth of Codonopsis lanceolata showed superior tendency in the treatment of mixed organic matter and fermentation cake. Lancemacides, the major saponin of C. lanceolata, could be identified as foetidissimoside A, lancemaside A, lancemaside B, and lancemaside D. However, among them, quantitative analysis could not be conducted on foetidissimoside A due to its very low content, and lancemaside A was the most abundant saponin in the root from all the treatments. The content of lancemaisde A according to organic fertilizer application showed the highest value of 0.12 mg g−1 in the treatment of mixed organic matter, followed by the fermentation cake, bacterial culture, non-treatment, rice husks and chemical fertilizer, in that order. The content of lancemaside B and D was very low compared to lancemaside A, and there was no difference among treatments. Therefore, it was verified that the growth of C. lanceolata was superior in application of mixed organic matter and fermentation cake, and the major saponin, lancemaside A, was also increased.

Background: This study aimed to investigate the effect of harvest time on the growth, yield characteristics and loganin content in Dipsacus asperoides Wall. Methods and Results: Dipsacus asperoides seedlings were planted within a nursery environment in early May 2015 and harvested in early, middle and late October 2015, and early November 2015. Harvest time did not result significant differences in the plant height, stem diameter, branch length, leaf width and aboveground dry weight moreover, no significant differences were observed in root length, number of roots and root diameter. However, the diameter of lateral roots was greater in the harvests from the late October and period thereafter. The highest values of root dry weight and yield were recorded in early November. Specifically, the yield significantly increased from 205 kg/10 a (index: 100) in early October to 358 kg/10 a (index: 175) in early November, in terms of root part weight. Loganin contents of D. asperoides differed significantly among harvest times raging from 0.0766% in early October to 0.1704% in late November, thereby showing an increasing trend in later harvest times. Conclusions: These results suggest that the optimum harvest time for D. asperoides is early November, when the yield is the highest. Harvest time significantly affected loganin contents, which constantly increased from early October until early November.

Background: The purpose of this study was to evaluate methods to reduce seeding expenses, thereby increasing farm income. This study investigated the effects of rain shelter controlled cultivation and adapted seeding times on the stable seed production of Scutellaria baicalensis Georgi. Methods and Results: Seed germination was conducted under 10 condition compose to control, water washing, cold storage at for 15 days, seed sterilization with a benomyl pesticides, hormone treated seed by submerging in 100 ppm with the cold storage at and , darkness in a covered petridish and illuminated with a 1,500 Lux lamp. There were three cultivation type, open cultivation with non-woven fabric mulching, cultivation with a vinyl covering and rain sheltered in a plastic greenhouse. Sowing dates were April 27, May 18, June 7 and June 28, 2013. Plants were spaced 10 cm apart in rows 30 cm apart. Mixed oil cake fertilizer, (12-10-10) was applied at . Conclusions: Optimum germination occured in darkness at and cold storage after submerging in . The highest seed yields () occurred in the plastic greenhouse for the April 25 sowing. The highest root yield (17%) was found on April 1, under greenhouse conditions.

Stem rot was observed on Ixeridium dentatum in Jinan-gun and Dangjin-gun, Korea during the growing seasons of 2016 and 2017. The first symptom was water-soaked lesions on the basal parts of stems. Lower leaves became wilted and blighted before the plants eventually died. White cottony mycelial mats and brown spherical or irregular sclerotia formed on the basal stem and adjacent soil surfaces. The optimal temperature for in vitro colony growth and sclerotia germination were 30 °C and 25 °C, respectively. The in vitro and morphological characteristics of the fungus were identical with those described for Sclerotium rolfsii. Phylogenetic analysis based on the internal transcribed spacer (ITS) region revealed that two isolates isolated from I. dentatum formed a monophyletic group with reference isolates of S. rolfsii. Koch’s postulates were satisfied for the same two S. rolfsii isolates, thereby confirming that the pathogen causes stem rot of I. detatum in Korea.

Background : The aim of the present study was to identify an effective physicochemical control method to reduce Fusarium species infestation in adlay (Coix lacryma-jobi L.) before and after harvesting. Methods and Results : We observed that prochloraz emusifiable concentrate and hexaconazol prochloraz emusifiable concentrate strongly inhibited the mycelial growth of 10 Fusarium species. Strong growth inhibitions and cell lysis were observed following treatment with 4% NaOCl solution. The total number of fungi detected were lower follwing treatment with thiophanatemethyl triflumizole wettable powder (), hexaconazol prochloraz emulsifiable concentrate (), carboxin thiram dustable powder () and prochloraz emulsifiable concentrate () than in the non-treated control (). The reduction of Fusarium fungi varies with the concentration and soaking time of NaOCl solution. Fungal detection was not observed after soaking in NaOCl solution for 24 h and harmful effects were not observed for plant growth by NaOCl after soacking for 6 - 12 h. Conclusion : Soaking seed for 6 - 12 h in 4% NaOCl could be an effective method of disinfectant treatment for the control of Fusarium fungi in adlay seeds.

Background : This study examined the effect of harvesting time on the growth, yield characteristics, and major beneficial components in Salvia miltiorrhiza. Methods and Results : Although plant height, stem diameter and branch length were not affected by harvesting time, the number of stems was highest when harvested in mid October. There were no differences in root length and thickness, however, the rhizome was thicker when it was harvested at the end of October or early November than when it was harvested in early and mid October. The dried root weight also showed a similar pattern. However, there was a statistically significant increase to 408 kg (16%) in the rhizome weight when in late October and a rise to 455 kg (29%) when harvested in early November. Harvest time had little effect on the content of the major component of S. miltiorrhiza. For example, salvianolic acid content rose from 9.42 to 9.64% with later harvest times, and tanshinone content was tended to be slightly more increased in mid October which S. miltiorrhiza has 0.22% tanshinon than in early October. Conclusions : According to these results, the optimum harvest time for S. miltiorrhiza is early November when plant or major component yields are hightest. There were no significant harvest time effects on the major beneficial components.

Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones. Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O--D-glucopyranoside was higher than that of other adventitious root samples. Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.

Background : Platycodon grandiflorum radix used as a therapeutic agent for lung and respiratory diseases in traditional Korean medicine. The saponin and sugar content in P. grandiflorum root is dependent on the drying temperature and method. In this study, we investigated saponins and antioxidant contents in dried P. grandiflorum roots either not cut or cut to different lengths. Methods and Results : P. grandiflorum roots were either cut to 0.5, 1.0 or 2.0 cm in length or left uncut. They were dried at and the effect of antioxidant substances, antioxidant ability, and saponin content were investigated. P. grandiflorum roots rapidly dried out when cut to short lengths. The saponin contents (platycodin D, polygalacin D, and deapioplatycodin D) was highest in the uncut P. grandiflorum roots. When the cut lengths were long, the levels of polyphenolic compound increased. However, 2,2'-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were higher in the samples cut to shorter lengths. Conclusions : These results suggested that the saponin and antioxidant content of P. grandiflorum roots could be maximized by controlling the drying rate of the roots via adjustments to the root cutting length.

One objective of this study was to compare the antioxidant activity from various organs of Artemisia species and identify new organs of Artemisia species with antioxidant activity. The various organs of the Artemisia species were analyzed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, which are widely used for evaluating antioxidant activity. A. annua, A. montana, A. stolonifera and A. gmelinii found to have strong antioxidant activity. These findings reinforce the potential of plants as valuable antioxidant sources. A second objective of this study was to compare the artemisinin content from various organs of Artemisia species and identify that a new organ of Artemisia species with high artemisinin content. Artemisinin is one of the most important phytochemicals found in the Artemisia species, and has been used for the treatment of malaria all over the world. Using high-performance liquid chromatography, we analyzed the artemisinin content from various organs of the Artemisia species. The roots of A. absinthium and A. rubripes howed high contents of artemisinin. This, roots of A. absinthium and A. rubripes are suggested as alternatives to A. annua as medicinal sources of artemisinin.

*Department of Herbal Crop Research, NIHHS, RDA, Eumsung 369-873, Korea.**Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan 340-702, Korea.ABSTRACT : This study was conducted to obtain the basic data related to seed characteristics and germination conditionsof the Coriandrum sativum L. at different storage condition and temperature. The shape of fruit was oval with light browncolor. Fruit was mericarp, biloculate, with one ovule in each locus. The length and width of seed were 1.37±0.067 ㎜ and0.52 ±0.039 ㎜, respectively. Weight of 1,000 seeds was 6.55±0.15 g. Seeds which were stored at room (15℃) and cold tem-perature (4℃) in vinyl container showed the highest germination rate (93.3%) under the room temperature germinationcondition. Percentage of germination in cold and freeze temperature was 20% and 0%, respectively. Germination rate ofroom storage seeds with paper container increased to about 91.3%, however, decreased in cold (4℃) and freeze storage (−20℃)with paper container (76.7% and 78.0%, respectively). Germination rate of seed in vinyl stock container was better thanthat of paper stock container. Germination rate of seeds stored at room temperature for 4 years (2010 - 2013) ranged from80.0% to 91.3%. Therefore, coriander seeds are expected to be stored at room temperature for increasing the germinationrate and keeping a long term.Key Words : Coriandrum sativum L., Germination Temperature, Seed Characteristics, Storage Condition

This study was conducted to improve the postharvest storage techniques of managing and storing seeds, to test qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthes japonica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperatures and durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germination rates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than those in Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12 ㎜ than those in Je-Cheon about 4.90 ㎜ and seed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areas were higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing tempera- tures and durations, they were not clearly different in between 4℃ and 25℃ and they also were gradually decreased with getting longer storing durations. The germination rates treated by plant growth regulators were higher with GA3 than those with Kinetin. The highest seed germination rate was appeared at 50 ppm of GA3. Comparing its rates with different seed priming, they were relatively higher with KNO3 than those with PEG6000. In protein expression patterns between before the germinating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination com- pared to those before the germination of seeds, especially 10 ~ 20 kDa. These results showing more and clear bands were more clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulators and seed primings, GA3 was better expression than those with Kinetin and KNO3 was better than those with PEG6000. More and clear bands were closely related to the germination rates of seeds and more detailed studies would be required.

This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of man- aging and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvest- ing seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of GA3 and Kinetin was affected to improve the seed germination. GA3 increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher ger- mination rate with increasing their levels, whereas KNO3 increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results show- ing more and clearer bands were positively related to the germination rates which were different by seed colors, storage tem- peratures and durations, and plant growth regulators and primings.

*Department of Herbal Crop Research, NIHHS, RDA, Eumseong 369-873, Korea.**Jeollanamdo Development Institute for Korean Traditional Medicine, Jangheung 529-851, Korea.ABSTRACT : This study was carried out to investigate the variation of growth and yield using different planting densitiesand vinyl mulching in S. miltiorrhiza cultivation. Top plant growth was observed in the 30 × 30 ㎝ planting density of S. mil-tiorrhiza;, plant height, leaves and branches were larger than in the other treatments. Root length, root diameter, and sup-porting roots of underground part were increased as planting density was wide, and dry root weight was increased in30 × 30 ㎝. However, yield was highest at 294㎏/10a in the planting density of 30× 10 ㎝. The use of 30-31㎝ white andblack vinyl mulching had no significant effect on the plant height of S. miltiorrhiza, as compared to non-mulching. However,the highest volume of leaves and plant weight was observed in plants using 24.7 black vinyl mulching. Dry underground rootweight was highest in black vinyl mulching at 21.7 g compared to 17.0 g for non-mulching. Yield per 10a increased by 28%using black vinyl mulching compared to non-mulching. Based on the results of this study, planting density of 30 × 10 ㎝ andblack vinyl mulching are the most suitable in the cultivation of S. miltiorrhiza.Key Words : Salvia miltiorrhiza , Planting Density, Mulching Materials, Root Yield

Powdery mildew symptoms were observed on the upper surface of leaves of Cynanchum auriculatum in Eumseong, Korea in October 2015. Microscopic observations and molecular characteristics determined this fungus as Podosphaera sp., which is reported for the first time on Cynanchum auriculatum.

This study was carried out to know the characteristics of flowering and bearing fruit, the optimum period, regions and methods for seed harvesting, the optimum temperatures for seed storage and germination, and the optimum period for sowing at nursery bed and seedling transplanting of Valeriana fauriei Briquet. The flowering and bearing fruit of Valeriana fauriei was developed from the before-year root. Optimum period for seed harvest of Valeriana fauriei was from late July to middle August, and optimum areas were the high elevated areas over 500 m above the sea level as Jinbu-myeon, Pyeongchang-gun, Gangwon-do. Using of net-bag for seed harvesting was the effective method to gather the full ripe seed, and bagging of net-bag was necessary from the season of middle May that was the flowering middle-stage. Germination rates don`t show the difference among the different temperatures of storage as approximately 41% at , or of seed storage temperatures. The optimum temperature range was in for seed germination at nursery bed. The optimum period for seed sowing at nursery bed was the late February, and the optimum period for seedling transplanting was the middle April.

This study were carried out to find bolting response of cultivation in different regions and to isolate FLC (FLOWERING LOCUS C) homologs in Angelica gigas Nakai. The mean temperature of different regions, ordering in altitude, were as follows: 100 m > 350 m > 530 m > 700 m. The largest amount of rainfall was occurred in the region of 350 m while the longest time of sunshine was occurred in the region of 100 m. The content of soil chemical properties in regions showed pH 6.2 ~ 7.4, T-N 0.17 ~ 26, organic mater , , exchangeable potassium and calcium and magnesium were 0.78 ~ 1.15, 3.9 ~ 10.0, . L5 line of A. gigas was occurred in bolting at all regions, but the bolting ratio was 60.0% in 700 m region with non-mulching treatment. Manchu of A. gigas was not occurred in bolting at all regions. The accumulation bolting ratio of L5 line by non-mulching was higher than that of mulching as 90.4% and 72.8% in 100 m region. The MADS-box transcription factor FLC is one of the well-known examples as a strong floral repressor. We decided to isolate FLC homologs from A. gigas as a starting point of flowering mechanism research of this plant. We have isolated two RT-PCR products which showed very high amino acid sequence homology to Arabidopsis FLC.