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The basic Helix-Loop-Helix (bHLH) superfamily is the most widespread family of transcription factors in eukaryotic organisms, which can activate the expression of genes by interacting with specific promoters in the genes. The bHLH transcription factors direct the development and metabolic process of plants, including flowering initiation and secondary metabolite production, by attaching to specific sites on their promoters. These transcription factors are essential for encouraging plant tolerance or the adjustment to harsh environmental conditions. The involvement of bHLH genes in anthocyanin formation in fleshy fruit-bearing plants, as well as the role of these genes in response to stimuli including drought, salt, and cold stress, are discussed in this article. New concepts and goals for the production of stress--tolerant fruit species are suggested. Furthermore, solid evidence for the critical role of bHLH genes in the growth and development, as well as anthocyanin biosynthesis in fleshy fruit plants, are also presented in this article. This review identifies several future research directions that can shed light on the roles of bHLH genes in fruit-bearing plants and will assist the use of these genes in efforts to breed fruit crop varieties that are more resistant to stress. Generally, there has been little research carried out on the role of bHLHs transcription factor family genes in fleshy fruit-bearing plant species and more in-depth studies are required to fully understand the diverse role of bHLH genes in these species. [ABSTRACT FROM AUTHOR]

Efficient protocols for callus induction and micro propagation of Saussurea costus (Falc.) Lipsch were developed and phytochemical diversity of wild and in-vitro propagated material was investigated. Brown and red compact callus was formed with frequency of 80–95%, 78–90%, 70–95% and 65–80% from seeds, leaf, petiole and root explants, respectively. MS media supplemented with BAP (2.0 mgL−1), NAA (1.0 mgL−1) and GA3 (0.25 mgL−1) best suited for multiple shoot buds initiation (82%), while maximum shoot length was formed on media with BAP (1.5 mgL−1), NAA (0.25 mgL−1) and Kinetin (0.5 mgL−1). Full strength media with IAA (0.5 mgL−1) along with IBA (0.5 mgL−1) resulted in early roots initiation. Similarly, maximum rooting (87.57%) and lateral roots formation (up to 6.76) was recorded on full strength media supplemented with BAP (0.5 mgL−1), IAA (0.5 mgL−1) and IBA (0.5 mgL−1). Survival rate of acclimatized plantlets in autoclaved garden soil, farmyard soil, and sand (2:1:1) was 87%. Phytochemical analysis revealed variations in biochemical contents i.e. maximum sugar (808.32 µM/ml), proline (48.14 mg/g), ascorbic acid (373.801 mM/g) and phenolic compounds (642.72 mgL−1) were recorded from callus cultured on different stress media. Nonetheless, highest flavenoids (59.892 mg/g) and anthocyanin contents (32.39 mg/kg) were observed in in-vitro propagated plants. GC–MS analysis of the callus ethyl acetate extracts revealed 24 different phytochemicals. The variability in secondary metabolites of both wild and propagated plants/callus is reported for the first time for this species. This study may provide a baseline for the conservation and sustainable utilization of S. costus with implications for isolation of unique and pharmacologically active compounds from callus or regenerated plantlets. [ABSTRACT FROM AUTHOR]

Eleven species: Oscillatoria acuminata Gomont, Oscillatoria princeps Vaucher, Oscillatoria tenius C.A. Agardh, Oscillatoria curviceps C.A. Agardh, Oscillatoria agardhii, Oscillatoria prolifica (Grev) Gomont, Oscillatoria chlorina Kutz ex Gomont, Oscillatoria proboscoidea Gomont, Oscillatoria margartifera (Kutz) Gomont, Oscillatoria perornata C.A. Agardh and Oscillatoria bornetii Zukal were identified from District Charsadda, Pakistan. [ABSTRACT FROM AUTHOR]

Fourteen genera with 27 species of 12 families and 7 orders of Chlorophyceae were identified. These include: Spirogyra (4 Spp.), Zygnema (3 Spp.), Cladophora (3 Spp.), Closterium (2 Spp.), Ulothrix (2 Spp.), and Stigeoclonium (2 Spp.), Pedistrum (2 Spp.), Oedogonium (2 Spp.), Coloecheate (2 Spp.), Chlorococcum (2 Spp.), and Cheatophora & Chlorella with one species each. [ABSTRACT FROM AUTHOR]

Plant species are distributed in different types of habitats, forming different communities driven by different sets of environmental variables. Here, we assessed potential plant communities along an altitudinal gradient and their associations with different environmental drivers in the unexplored Manoor Valley (Lesser Himalaya), Pakistan. We have implemented various ecological techniques and evaluated phytosociological attributes in three randomly selected 50 m-transects within each stand (a total of 133) during different seasons for four years (2015–2018). This phytosociological exploration reported 354 plant species representing 93 different families. The results revealed that the Therophytic life form class dominated the flora, whereas Nanophyll dominated the leaf size spectra. There were a total of twelve plant communities identified, ranging from the lowest elevations to the alpine meadows and cold deserts. The maximum number of species were found in Cedrus–Pinus–Parrotiopsis community (197 species), in the middle altitudinal ranges (2292–3168 m). Our results showed that at high altitudes, species richness was reduced, whereas an increase in soil nutrients was linked to progression in vegetation indicators. We also found different clusters of species with similar habitats. Our study clearly shows how altitudinal variables can cluster different plant communities according to different microclimates. Studies such as ours are paramount to better understanding how environmental factors influence ecological and evolutionary aspects. [ABSTRACT FROM AUTHOR]

The ecotonal zones support populations that are acclimated to changing, fluctuating, and unstable conditions, and as a result, these populations are better equipped to adjust to expected change. In this context, a hypothesis was tested that there must be vegetation dominated by unique indicator plant species under the influence of ecological gradients in the ecotonal zone of Manoor Valley (northwestern Himalaya), Pakistan. Keeping the aforementioned hypothesis in mind, detailed field studies were conducted during different seasons in 2015-18. Line transect sampling and phytosociological characteristics (density, frequency, cover, and their relative values and Importance Value) were implemented as ecological methods. This investigation documented 97 plant species recorded from seven sampling sites. The community distribution modelling revealed that the ecological variables separate the seven sampling sites into two major plant communities (Indigofera-Parrotiopsis-Bistorta and Ziziphus-Leptopus-Quercus) recognized by TWINSPAN. The IBP communities showed a positive and significant correlation with altitude (1789.6–1896.3 m), sandy soil texture with a slightly acidic pH (6.4–6.5), and higher phosphorous (9–13 mg kg−1). In contrast with this, the ZLQ community was recognized on the southern slope under the strong influence of high electrical conductivity (2.82–5.4 dsm−1), organic matter (1.08–1.25%), calcium carbonate (5.8–7.6 mg kg−1), potassium (202–220 mg kg−1), and temperature (28.8–31.8 °C). Hence, both communities were found on opposite axes with clear differences based on the ecological gradients. NMDS clustered different species with similar habitats and different stands with common species, showing that plant species and stands were in a linear combination with ecological gradients. The IPB community has the maximum number of plant species (87 species), Shannon value (H' = 4), Simpson value (0.98), and Pielou's evenness value (0.96). Thus, the multivariate approaches revealed unique vegetation with sharp boundaries between communities which might be due to abrupt environmental changes. [ABSTRACT FROM AUTHOR]

The development of vegetation communities is typically influenced by their response to variation in environmental, geographic and as well as physiographic gradients. The current study was planned to evaluate the influence of environmental gradients upon the structure of plant communities and to highlight their respective indicators in subalpine pastures of the Himalaya, Pakistan. In relation to this aim, ecological techniques were used following the Line transect (50 m) method to quantify the vegetation structure of the study area. Soil samples were collected from each sampling site and edaphic gradients were examined using standard protocols. Weather station data (Kestrel 4000) was used to determine the climatic gradients, GPS data was used to record the geographic and physiographic gradients. PCORD software was used to recognize communities through two-way indicator species analysis (TWINSPAN), R and CANOCO software was employed for ordination analysis to find variation directories of different plant species. A total of 56 plant species, recorded from 21 sampling sites, were grouped into four plant communities with the help of environmental gradients. The highest index of similarity was recorded between the Bergenia-Sibbaldia-Rheum and Sibbaldia-Rheum-Bergenia communities and highest dissimilarity between Bergenia-Sibbaldia-Rheum and Juniperus-Sibbaldia-Poa communities. The highest number of plant species (50 species), maximum alpha diversity (H′ = 3.38) and beta diversity was reported in Sibbaldia-Rheum-Bergenia community (0.95), but Pielou's evenness was highest (0.89) in Juniperus-Sibbaldia-Poa among all recorded communities. Besides, the edaphic (i.e. organic matter, phosphorous, pH and soil texture) and climatic factors (temperature, humidity) were the strong environmental gradients that were responsible for structuring and hosting the diverse plant communities in subalpine meadows. Techniques adapted in the current study for identification of vegetation indicators could further be used for conservation management. • Natural variation in the ecological gradients drives a change in the vegetation structure. • Application of multivariate approaches for the identification of indicator species. • Environmental gradients are responsible for structuring plant communities and their indicators in subalpine pastures. • Techniques adopted in this study could further be used for conservation management. [ABSTRACT FROM AUTHOR]

In spite of the remarkable achievements in the healthcare sector over recent decades, inequities in accessibility and affordability of these facilities coexist throughout Pakistan. Thus, we aimed to explore and document the cultural knowledge of herbal teas used medicinally by the local community members of Manoor Valley, Pakistan. Field investigations were undertaken during the summer season of 2015–2017, and cultural practices of medicinal plant usage for treating various ailments were gathered through interviews of the local inhabitants. Ethnomedicinal insights of the medicinal plants used in herbal teas were gained with different indexes. Our results revealed 27 plant species, comprising of herbs (70%), shrubs (26%), and trees (4%), which were used for treating 21 diseases. Plants belonged to 18 families: Asteraceae and Lamiaceae were the leading families used for treating diseases. Diarrhea and gas troubles were the most frequent diseases. Based on indexes values, Cannabissativa was the dominant species used. The results revealed that 57% of medicinal uses are new to literature. This ethnomedicinal study is providing the first insights into the traditional medication system of Lesser Himalaya, Pakistan, through ethnomedicinal teas. [ABSTRACT FROM AUTHOR]