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6 results on '"Dure LS"'

1. Action of 3'd adenosine (cordycepin) and 3'd cytidine on the translation of the stored mRNA of cotton cotyledons.

Author

Walbot V, Capdevila A, and Dure LS 3rd

Subjects
Adenosine pharmacology, Carbon Radioisotopes, Carboxypeptidases biosynthesis, Cell Division, Cytosine pharmacology, Dactinomycin pharmacology, Gossypium drug effects, Gossypium metabolism, Leucine metabolism, Plant Proteins biosynthesis, Plants drug effects, Seeds drug effects, Seeds metabolism, Time Factors, Adenosine analogs & derivatives, Cytosine analogs & derivatives, Plants metabolism, Protein Biosynthesis drug effects, RNA, Messenger metabolism
Published
1974
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2. Cotton messenger RNA sequences exist in both polyadenylated and nonpolyadenylated forms.

Author

Galau GA, Legocki AB, Greenway SC, and Dure LS 3rd

Subjects
Molecular Weight, Nucleic Acid Hybridization, Poly A isolation & purification, Polyribosomes metabolism, Protein Biosynthesis, RNA, Messenger isolation & purification, Triticum metabolism, Gossypium metabolism, Plants metabolism, Poly A metabolism, RNA, Messenger metabolism
Abstract

The physical characteristics and sequence content of polyadenylated and nonpolyadenylated messenger RNAs were determined in total and polysomal RNAs isolated from cotton cotyledons after 24 h of germination. RNAs were fractionated on the basis of their poly(A) content by passage through poly(U)-agarose. The mass average size of poly(A) is 112 +/- 4 nucleotides in total RNA and 81 +/- 3 nucleotides in polysomal RNA. Less than 2% of the poly(A) tracts are between 5-15 nucleotides in length. The mass average length of recovered polyadenylated mRNAs from either RNA is 1600 nucleotides. In vitro translation of the RNAs in the wheat germ system and hybridization of the RNAs with complementary DNAs confirmed the physical estimates of their absolute amounts. About 3.3% of the high molecular weight RNA in the tissue is mRNA, of which 40% is on polysomes. Abut 45% of the polysomal mRNA and 75% of the nonpolysomal mRNA is polyadenylated. These mRNAs are transcribed from nonrepetitive DNA. Both techniques unambiguously demonstrate that all mRNA populations contain the same sequences in about the same relative concentration. At this point of development in this tissue, differential polyadenylation or entry into polysomes cannot be specific for particular mRNA sequences.

Published
1981

3. Differential effects of 3'-deoxy nucleosides on RNA synthesis in cotton cotyledons.

Author

Harris B and Dure LS 3rd

Subjects
Adenine Nucleotides metabolism, Adenosine metabolism, Cell Nucleus metabolism, Chromatography, Gel, Deoxyadenosines pharmacology, Deoxycytidine pharmacology, Electrophoresis, Polyacrylamide Gel, Gossypium metabolism, Polynucleotides analysis, Seeds metabolism, DNA Nucleotidyltransferases antagonists & inhibitors, Deoxyribonucleosides pharmacology, Plants metabolism, RNA biosynthesis
Published
1974
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4. The developmental biochemistry of cottonseed embryogenesis and germination. 3. Regulation of the biosynthesis of enzymes utilized in germination.

Author

Ihle JN and Dure LS 3rd

Subjects
Cycloheximide pharmacology, DNA biosynthesis, Dactinomycin pharmacology, Fluorouracil pharmacology, Gibberellins pharmacology, Gossypium drug effects, Gossypium enzymology, Gossypium growth & development, Gossypium metabolism, Isocitrates, Models, Biological, Phosphates metabolism, Phosphorus Isotopes, Plant Development, Plant Growth Regulators pharmacology, Plants drug effects, Plants metabolism, RNA biosynthesis, RNA, Messenger biosynthesis, Carboxypeptidases biosynthesis, Lyases biosynthesis, Plants enzymology
Published
1972

5. The developmental biochemistry of cottonseed embryogenesis and germination. II. Catalytic properties of the cotton carboxypeptidase.

Author

Ihle JN and Dure LS 3rd

Subjects
Binding Sites, Carbon Isotopes, Catalysis, Chymotrypsin, Electrophoresis, Disc, Electrophoresis, Paper, Esters, Gossypium enzymology, Hydrolysis, Kinetics, Macromolecular Substances, Peptides isolation & purification, Phosphorus Isotopes, Protein Binding, Protein Denaturation, Proteins, Serine, Sodium Dodecyl Sulfate, Structure-Activity Relationship, Sulfhydryl Reagents, Carboxypeptidases analysis, Carboxypeptidases antagonists & inhibitors, Plants enzymology
Published
1972

6. The developmental biochemistry of cottonseed embryogenesis and germination. I. Purification and properties of a carboxypeptidase from germinating cotyledons.

Author

Ihle JN and Dure LS 3rd

Subjects
Amino Acids analysis, Amino Acids metabolism, Arginine, Benzoates, Carbon Isotopes, Chemical Phenomena, Chemistry, Physical, Chromatography, DEAE-Cellulose, Chromatography, Gel, Dialysis, Drug Stability, Electrophoresis, Disc, Gossypium enzymology, Gossypium growth & development, Hydrogen-Ion Concentration, Macromolecular Substances, Molecular Weight, Plant Development, Plants metabolism, Sodium Dodecyl Sulfate, Ultracentrifugation, Carboxypeptidases analysis, Carboxypeptidases biosynthesis, Carboxypeptidases isolation & purification, Plants enzymology
Published
1972

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