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Non-thermal atmospheric-pressure plasma (NTAPP) has been widely studied for clinical applications, e.g., disinfection, wound healing, cancer therapy, hemostasis, and bone regeneration. It is being revealed that the physical and chemical actions of plasma have enabled these clinical applications. Based on our previous report regarding plasma-stimulated bone regeneration, this study focused on Achilles tendon repair by NTAPP. This is the first study to reveal that exposure to NTAPP can accelerate Achilles tendon repair using a well-established Achilles tendon injury rat model. Histological evaluation using the Stoll's and histological scores showed a significant improvement at 2 and 4 weeks, with type I collagen content being substantial at the early time point of 2 weeks post-surgery. Notably, the replacement of type III collagen with type I collagen occurred more frequently in the plasma-treated groups at the early stage of repair. Tensile strength test results showed that the maximum breaking strength in the plasma-treated group at two weeks was significantly higher than that in the untreated group. Overall, our results indicate that a single event of NTAPP treatment during the surgery can contribute to an early recovery of an injured tendon., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Nakazawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Non-thermal atmospheric-pressure plasma (NTAPP) is attracting widespread interest for use in medical applications. The tissue repair capacity of NTAPP has been reported in various fields; however, little is known about its effect on fracture healing. Non-union or delayed union after a fracture is a clinical challenge. In this study, we aimed to investigate how NTAPP irradiation promotes fracture healing in a non-union fracture model and its underlying mechanism, in vitro and in vivo. For the in vivo study, we created normal and non-union fracture models in LEW/SsNSlc rats to investigate the effects of NTAPP. To create a fracture, a transverse osteotomy was performed in the middle of the femoral shaft. To induce the non-union fracture model, the periosteum surrounding the fracture site was cauterized after a normal fracture model was created. The normal fracture model showed no significant difference in bone healing between the control and NTAPP-treated groups. The non-union fracture model demonstrated that the NTAPP-treated group showed consistent improvement in fracture healing. Histological and biomechanical assessments confirmed the fracture healing. The in vitro study using pre-osteoblastic MC3T3-E1 cells demonstrated that NTAPP irradiation under specific conditions did not reduce cell proliferation but did enhance osteoblastic differentiation. Overall, these results suggest that NTAPP is a novel approach to the treatment of bone fractures., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Saito et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Non-thermal atmospheric gas plasma (AGP) exhibits cytotoxicity against malignant cells with minimal cytotoxicity toward normal cells. However, the mechanisms of its tumor-selective cytotoxicity remain unclear. Here we report that AGP-activated medium increases caspase-independent cell death and mitochondrial network collapse in a panel of human cancer cells, but not in non-transformed cells. AGP irradiation stimulated reactive oxygen species (ROS) generation in AGP-activated medium, and in turn the resulting stable ROS, most likely hydrogen peroxide (H2O2), activated intracellular ROS generation and mitochondrial ROS (mROS) accumulation. Culture in AGP-activated medium resulted in cell death and excessive mitochondrial fragmentation and clustering, and these responses were inhibited by ROS scavengers. AGP-activated medium also increased dynamin-related protein 1-dependent mitochondrial fission in a tumor-specific manner, and H2O2 administration showed similar effects. Moreover, the vulnerability of tumor cells to mitochondrial network collapse appeared to result from their higher sensitivity to mROS accumulation induced by AGP-activated medium or H2O2. The present findings expand our previous observations on death receptor-mediated tumor-selective cell killing and reinforce the importance of mitochondrial network remodeling as a powerful target for tumor-selective cancer treatment.

Abstract: New ion cyclotron range of frequencies (ICRF) antennas have just been installed in the large helical device (LHD). These side-by-side ICRF antennas are symmetrical and designed to launch fast waves with various wave numbers parallel to the magnetic field line. The wave number can be controlled by changing the current phase on the straps; however, the mutual coupling between antennas changes antenna impedances, even if the plasma parameters are constant, leading to an increase in the reflected power. In addition to the current phase control, impedance matching devices must be tuned for the protection of tetrode tubes and efficient power injection. For this purpose, the impedance matrix of ICRF antennas must be determined, and it can be deduced from the forward and reflected waves at the outlet of the power amplifier by assuming geometric symmetry and reciprocity of the antennas. Using half-scale antennas, we successfully demonstrated simultaneous impedance matching and current phase control. [Copyright &y& Elsevier]

Achieving steady-state plasma operation at high plasma temperatures is one of the important goals of worldwide magnetic fusion research. A high temperature of approximately 2 keV, and steady-state plasma-sustainment operation of the Large Helical Device (LHD) [O. Motojima, K. Akaishi, H. Chikaraishi et al., Nucl. Fusion 40, 599 (2000)] is reported. High-temperature plasmas were created and maintained for more than 30 min with a world record injected heating power of 1.3 GJ. The three-dimensional heat-deposition profile of the LHD helical divertor was modified and during long-pulse discharges it effectively dispersed the heat load using a magnetic-axis swing technique developed at the LHD. A sweep of only 3 cm of the major radius of the magnetic axis position (less than 1% of the major radius of the LHD) was enough to disperse the divertor heat load. The modification of the heat-load profile was explained well by field-line tracing. The steady-state plasma was heated and sustained mainly by hydrogen minority ion heating using ion cyclotron range of frequencies. The operation lasted until a sudden increase of radiation loss occurred, presumably because of wall metal flakes dropping into the plasma. The sustained line-averaged electron density was approximately 0.7–0.8×1019 m-3. The average input power was 680 kW, and the plasma duration was 31 min 45 s. This successful long operation shows that the heliotron configuration has a high potential as a steady-state fusion reactor. [ABSTRACT FROM AUTHOR]

The neoclassical magnetohydrodynamic equilibrium of reversed-field pinch (RFP) is self-consistently solved considering the self-induced plasma current I[lowercase_phi_synonym]SI in a reactor relevant parameter regime. The current I[lowercase_phi_synonym]SI consists of bootstrap currents due to α particles as well as the bulk plasma, where the conventional neoclassical transport theory is applied without considering the finite banana-width effect, Pfirsch–Schluter current, and diamagnetic current. The neoclassical effect enhances I[lowercase_phi_synonym]SI in a low-aspect-ratio RFP (aspect ratio A=2), when β is high. An I[lowercase_phi_synonym]SI as high as 94.4% of the plasma current in the equilibrium with ellipticity κ=1.4 and triangularity δ=0.4 is obtained with a flat pressure profile, which provides a toroidal β of βt=63% (volume-averaged β, 〈β〉=66%) stable against both the ideal kink mode and Mercier mode. This dominant self-induced current makes the steady-state approach feasible. Its hollow current profile gives a strong magnetic shear and a high-stability β. [ABSTRACT FROM AUTHOR]

Ion cyclotron range of frequency (ICRF) heating experiments on the Large Helical Device (LHD) [O. Motojima et al. Fus. Eng. Des. 20, 3 (1993)] achieved significant advances during the third experimental campaign carried out in 1999. They showed significant results in two heating modes; these are modes of the ICH-sustained plasma with large plasma stored energy and the neutral beam injection (NBI) plasma under additional heating. A long-pulse operation of more than 1 minute was achieved ata level of 1 MW. The characteristics of the ICRF heated plasma are the same as those of the NBI heated plasma. The energy confinement time is longer than that of International Stellarator Scaling 95. Three keys to successful ICRF heating are as follows: (1) an increase in the magnetic field strength, (2) the employment of an inward shift of the magnetic axis, (3) the installation of actively cooled graphite plates along the divertor legs. Highly energetic protons accelerated by the ICRF electric field were experimentally observed in the energy range from 30 to 250 keV and the tail temperature depended on the energy balance between the wave heating and the electron drag. The transfer efficiency from the high energy ions to the bulk plasma was deduced from the increase in the energy confinement time due to the high energy ions in the lower density discharge, which agrees fairly well with the result obtained by the Monte Carlo simulation. The transfer efficiency is expected to be 95% at an electron density of more than n[sub e] = 5.0 × 10[sup 19] m[sup -3] even in the high power heating of 10 MW. The accumulation of impurities, e.g., FeXVI and OV was not observed in high rf power and long pulse operation. The well-defined divertor intrinsic to LHD is believed to be useful in reducing the impurity influx. [ABSTRACT FROM AUTHOR]

Asserts that a reversed-field pinch (RFP) dynamo uniformly generates most of the toroidal flux in a RFP plasma with a high magnetic Reynolds number. Features of the global dynamo and magnetic fluctuations; Global dynamo and Taylor's theory; Mechanism of the global dynamo.

Abstract: A three-dimensional neutral particle transport simulation code is applied to identify a source of the hydrogen outgas in a LHD long pulse discharge by ICRF heating. Radiation collapse induced by uncontrollable plasma density rise terminated the long pulse discharge. Toroidally non-uniform increases in the Hα emission and divertor temperature were observed. A CCD camera detected local heating of a vertically installed divertor plate, which is consistent with the strike points of the trajectories of protons accelerated by ICRF waves. The neutral particle transport simulation with the proton trajectory analyses shows that outgas from the divertor plates heated by the protons is necessary for explaining the observed toroidal non-uniform distribution of the Hα emission in the long pulse discharge. [Copyright &y& Elsevier]

The wall conditioning using radio frequency (RF) plasma was carried out using Ion Cyclotron Range of Frequency (ICRF) heating and/or Electron Cyclotron Resonance Heating (ECRH) with the working gas of helium under the established confinement magnetic field. After sufficient numbers of repetitive wall discharge conditioning (DC, ICDC for ICRF and ECDC for ECRH), the formation of the parabolic electron density profile and the increase of the central ion temperature T i were observed. There was no difference in the attained central T i of the NBI discharge just after the conditioning using a similar value of input energy regardless of the applied RF source. Thus, it is concluded that both ICDC and the ECDC are effective for the higher T i plasma production under the established magnetic field in the Large Helical Device. The effective RF wall conditioning scenarios are also investigated for high T i plasma production. [ABSTRACT FROM AUTHOR]

Abstract: The Ion Cyclotron Heating and Current Drive (IC H&CD) system for ITER will provide 20MW to the plasma. The associated Radio Frequency (RF) source system has to be compliant with all operation modes foreseen in that frame. Their specifications are fully described in this paper and constraints on IC RF source components are detailed, in particular concerning the final stage tube of the amplifier. Results of tests performed under a collaborative work at the National Institute for Fusion Science (NIFS) facility are presented. Consequences on the procurement process by ITER India (II) are deduced. [Copyright &y& Elsevier]