1. Effect of a plasma synthesized polypyrrole coverage on polylactic acid/hydroxyapatite scaffolds for bone tissue engineering
- Author
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Omar E. Uribe-Juarez, María G. Flores-Sánchez, Roberto Olayo, Greta P. Ortiz-Vázquez, Flor I. Vivar-Velázquez, Diego R. Esquiliano-Rendon, Juan Morales-Corona, Atlántida M. Raya-Rivera, and Nancy C. Islas-Arteaga
- Subjects
Male ,Materials science ,Bone Regeneration ,Plasma Gases ,Polymers ,Polyesters ,0206 medical engineering ,Biomedical Engineering ,02 engineering and technology ,Polypyrrole ,Bone and Bones ,Biomaterials ,chemistry.chemical_compound ,Polylactic acid ,Bone cell ,Animals ,MTT assay ,Pyrroles ,chemistry.chemical_classification ,Tissue Engineering ,Tissue Scaffolds ,Metals and Alloys ,Bone Marrow Stem Cell ,Polymer ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Plasma polymerization ,Electrospinning ,Durapatite ,chemistry ,Chemical engineering ,Ceramics and Composites ,Rabbits ,0210 nano-technology - Abstract
Composite biomaterials are solids that contain two or more different materials, combining the properties of their components to restore or improve the function of tissues. In this study, we report the generation of electrospun matrices with osteoconductive properties and porosity using the combination of a biodegradable polyester, polylactic acid (PLA), and hydroxyapatite (HA). Additionally, we report the effects of modifying these matrices through plasma polymerization of pyrrole on the growth and osteogenic differentiation of rabbit bone marrow stem cells. Cells were isolated, seeded and cultured on biomaterials for periods between 7 and 28 days. The matrices we obtained were formed by nano and microfibers containing up to 35.7 wt% HA, presenting a variety of apparent pore sizes to allow for the passage of nutrients to bone cells. Scanning electron microscopy showed that the fibers were coated with polypyrrole doped with iodine, and MTT assay demonstrated this increased cell proliferation and significantly improved cell viability due to the adhesive properties of the polymer. Our results show that PLA/HA/Pyrrole/Iodine matrices are favorable for bone tissue engineering.
- Published
- 2021