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5 results on '"Ovchinnikova LP"'

1. [Induction of directed mutations in the tetracycline resistance gene of plasmid pBR 322 using complementary single-stranded DNA fragments carrying alkylating groups].

Author

Mazin AV, Dianov GL, Ovchinnikova LP, and Salganik RI

Subjects
Escherichia coli drug effects, Escherichia coli genetics, Formamides pharmacology, Nucleic Acid Hybridization drug effects, Alkylating Agents pharmacology, DNA pharmacology, DNA, Single-Stranded pharmacology, Genes, Bacterial drug effects, Mutation, Plasmids drug effects, Tetracycline antagonists & inhibitors
Published
1983

2. [Induction of a wide spectrum of recombinations resulting from limited site-directed damage to plasmid DNA by chemical mutagens].

Author

Salganik RI, Dianov GL, Vasiunina EA, Ovchinnikova LP, and Sinitsyna OI

Subjects
DNA, Circular genetics, Escherichia coli drug effects, Hydroxylamines pharmacology, Sulfuric Acid Esters pharmacology, T-Phages genetics, Attachment Sites, Microbiological drug effects, DNA, Bacterial genetics, Escherichia coli genetics, Lysogeny drug effects, Mutagens pharmacology, Plasmids drug effects, Recombination, Genetic drug effects
Published
1984

3. [Induction of repetitive nucleotide sequences in the Tcr gene of plasmid pBR 322 resulting from gene-directed mutagenesis].

Author

Salganik RI, Mazin AV, Dianov GL, and Ovchinnikova LP

Subjects
Alkylating Agents pharmacology, Base Sequence drug effects, DNA, Bacterial genetics, DNA, Single-Stranded genetics, Drug Resistance, Microbial, Escherichia coli drug effects, Escherichia coli genetics, Nitrogen Mustard Compounds pharmacology, Gene Expression Regulation drug effects, Genes, Bacterial drug effects, Mutation, Plasmids drug effects, Repetitive Sequences, Nucleic Acid drug effects, Tetracycline antagonists & inhibitors
Published
1984

4. The molecular basis of the origin of complete and mosaic mutants.

Author

Dianov GL, Vasyunina EA, Ovchinnikova LP, Sinitsina OI, and Salganik RI

Subjects
DNA Repair, Escherichia coli genetics, Mosaicism, Transformation, Genetic, DNA, Bacterial genetics, Mutation, Plasmids
Abstract

To study the molecular basis of the origin of complete and mosaic mutants, pBR322 plasmids with damage to one or both DNA strands were constructed by limited chemical modification of plasmid DNA. Damage to one strand of DNA resulted in the induction of predominantly mosaic mutants. Data were obtained indicating that complete mutations arise as a result of damage to both strands in the region of the mutated gene.

Published
1986
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5. [Introduction of new DNA sequences into previously selected regions of a plasmid genome by means of the formation of heteroduplexes].

Author

Saparbaev MK, Mazin AV, Ovchinnikova LP, Dianov GL, and Salganik RI

Subjects
Base Sequence, Cloning, Molecular, DNA Restriction Enzymes, Escherichia coli genetics, DNA, Recombinant, Nucleic Acid Heteroduplexes, Nucleic Acid Hybridization, Plasmids
Abstract

A new method for obtaining the recombinant DNA based on heteroduplex-initiated site-directed insertion of alien nucleotide sequences is proposed. To generate a single-stranded region, plasmid DNA was nicked with restriction endonuclease in the presence of ethidium bromide with subsequent exonuclease III controlled digestion. The inserted DNA sequences flanked by nucleotide sequences complementary to single-stranded region were annealed with plasmid DNA and E. coli cells were transformed by the resulting heteroduplex molecules. The presented data show the possibility to insert as many as 200 nucleotides. The yield of recombinant DNA varied from 16 to 0.7% as the number of nucleotides inserted correspondingly varied from 15 to 200. The site of insertion does not depend crucially on the localization of the restriction site used.

Published
1988

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