1. CD8+ T cell activation by murine erythroblasts infected with malaria parasites.
- Author
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Imai, Takashi, Ishida, Hidekazu, Suzue, Kazutomo, Hirai, Makoto, Taniguchi, Tomoyo, Okada, Hiroko, Suzuki, Tomohisa, Shimokawa, Chikako, and Hisaeda, Hajime
- Subjects
CD8 antigen ,PLASMODIUM ,PLASMODIUM falciparum ,PLASMODIUM vivax ,MALARIA - Abstract
Recent studies show that some human malaria parasite species Plasmodium falciparum and P.vivax parasitize erythroblasts; however, the biological and clinical significance of this is unclear. To investigate further, we generated a rodent malaria parasite (P. yoelii 17XNL) expressing GFP-ovalbumin (OVA). Its infectivity to erythroblasts was confirmed and parasitized erythroblasts were capable of initiating malaria infections. Experiments showed that MHC class I molecules were highly expressed on parasitized erythroblasts. As CD8
+ T cells recognize MHC class I and peptide complexes on target cells and are involved in protection or pathology against malaria, we examined whether erythroblasts are targeted by CD8+ T cells. Purified non-parasitized erythroblasts pulsed with OVA peptides were recognized by OVA-specific CD8+ T cells. Crucially, parasitized erythroblasts isolated from GFP-OVA-, but not GFP- infected-mice, activated OT-I CD8+ T cells, indicating that CD8+ T cells recognize parasitized erythroblasts in an antigen-specific manner. [ABSTRACT FROM AUTHOR]- Published
- 2013
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