1. Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe.
- Author
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Zhang T, Tian F, Long L, Liu J, and Wu X
- Subjects
- Catalysis, Humans, Immunoassay methods, Metal Nanoparticles chemistry, Rubella immunology, Rubella virology, Antigens, Viral immunology, Gold chemistry, Horseradish Peroxidase metabolism, Nanotubes chemistry, Platinum chemistry, Rubella diagnosis, Rubella virus immunology
- Abstract
Background: As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools., Purpose: Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen., Method: Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus., Results: Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity., Conclusion: Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions., Competing Interests: Disclosure The authors report no conflicts of interest in this work.
- Published
- 2018
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