1. Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix.
- Author
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Kim IG, Gil CH, Seo J, Park SJ, Subbiah R, Jung TH, Kim JS, Jeong YH, Chung HM, Lee JH, Lee MR, Moon SH, and Park K
- Subjects
- Animals, Cell Adhesion, Cell Culture Techniques, Cell Movement, Cell Proliferation, Epithelial-Mesenchymal Transition, Humans, Mice, NIH 3T3 Cells, Cell Engineering, Extracellular Matrix metabolism, Fibroblasts cytology, Mechanotransduction, Cellular, Pluripotent Stem Cells cytology
- Abstract
Cell-derived matrices (CDM) are becoming an attractive alternative to conventional biological scaffolding platforms due to its unique ability to closely recapitulate a native extracellular matrix (ECM) de novo. Although cell-substrate interactions are recognized to be principal in regulating stem cell behavior, very few studies have documented the acclimation of human pluripotent stem cells (hPSCs) on pristine and altered cell-derived matrices. Here, we investigate crosslink-induced mechanotransduction of hPSCs cultivated on decellularized fibroblast-derived matrices (FDM) to explore cell adhesion, growth, migration, and pluripotency in various biological landscapes. The results showed either substrate-mediated induction or inhibition of the Epithelial-Mesenchymal-Transition (EMT) program, strongly suggesting that FDM stiffness can be a dominant factor in mediating hPSC plasticity. We further propose an optimal FDM substratum intended for long-term hPSC cultivation in a feeder-free niche-like microenvironment. This study carries significant implications for hPSC cultivation and encourages more in-depth studies towards the fundamentals of hPSC-CDM interactions., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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