1. Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury.
- Author
-
Wong CK, Luk HK, Lai WH, Lau YM, Zhang RR, Wong AC, Lo GC, Chan KH, Hung IF, Tse HF, Woo PC, Lau SK, and Siu CW
- Subjects
- Angiotensin-Converting Enzyme 2, Betacoronavirus genetics, COVID-19, Cell Survival, Cells, Cultured, Coronavirus Infections metabolism, Coronavirus Infections virology, Coronavirus Nucleocapsid Proteins, Cytokines metabolism, Cytopathogenic Effect, Viral, Drug Evaluation, Preclinical methods, Humans, Induced Pluripotent Stem Cells metabolism, Myocarditis metabolism, Myocarditis virology, Myocytes, Cardiac metabolism, Nucleocapsid Proteins metabolism, Pandemics, Peptidyl-Dipeptidase A metabolism, Phosphoproteins, Pneumonia, Viral metabolism, Pneumonia, Viral virology, Reverse Transcriptase Polymerase Chain Reaction, SARS-CoV-2, Virus Replication, Betacoronavirus metabolism, Coronavirus Infections complications, Induced Pluripotent Stem Cells virology, Myocarditis complications, Myocytes, Cardiac virology, Pneumonia, Viral complications
- Abstract
Background: SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition., Methods and results: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated., Conclusions: Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.
- Published
- 2020
- Full Text
- View/download PDF