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Pollution of the environment by petroleum products is a global problem, since its extensive exploitation in broad areas. Microorganisms can degrade or transform toxic, petroleum derivates into harmless products with high efficiency. This study aimed to investigate the biodegradation process of petroleum hydrocarbons in contaminated sediment using indigenous and a combination of indigenous and allochthonous microorganisms. The allochthonous microorganisms that were added belonged to the genera Acinetobacter and Citrobacter. The experiment lasted two weeks, and the degradation process took place in a closed vessel on a Micro-Oxymax respirometer. Total petroleum hydrocarbons (TPH) were extracted and determined using a gas chromatograph with a flame ionization detector. The number of microorganisms at the beginning and end of the experiment was also determined. The results on the respirometer and gas chromatography indicated that the microorganisms were metabolically active. Indigenous microorganisms reduce the amount of TPH by 32.0% and the combination of indigenous-allochthonous microorganisms by 33.6%. The results showed that there is no significant difference between the application of autochthonous and the combination of indigenous-allochthonous microorganisms for the reduction of TPH in contaminated sediment, suggesting a high potential of autochthonous microorganisms for bioremediation processes.

Zagađenje vode, zemljišta i sedimenta naftom i njenim derivatima, može se javiti tokom eksploatacije, transporta i skladištenja i predstavlja rizik za životnu sredinu i ljudsko zdravlje. Bioremedijacija je zelena tehnologija kojom se uklanja zagađenje iz kontaminirane životne sredine upotrebom mikroorganizama. U ovom radu su praćene promene u sadržaju mineralnih ulja i aktivnost mikroorganizama koji razgrađuju ugljovodonike tokom procesa bioremedijacije koji je trajao 210 dana. Rezultati ukazuju da mikroorganizmi koji razgrađuju ugljovodonike sa visokom efikasnošću degraduju mineralna ulja. Na osnovu količine mineralnog ulja (smanjena za 63,82 %), stepena degradacije i udela mikroorganizama naftnih degradera u ukupnom broju mikroorganizama, može se zaključiti da su faza adaptacije konzorcijuma i faza intenzivne razgradnje bile u periodu od 0. do 90. dana, praćene dalje sporijom fazom razgradnje u periodu od 90. do 210. dana. Pollution of water, soil and sediment with petroleum and its products may occur during the exploitation, transportation and storage and it poses a risk to the environment and human health. Bioremediation is a green technology that can remove pollution from contaminated environment using microorganisms. In this paper changes in the content of mineral oil and the activity of hydrocarbon degraders during remediation process that lasted 210 days were monitored. The results indicate that hydrocarbon degrading microorganisms with high efficiency have biodegraded mineral oil. Based on the amount of mineral oil (decreased for 63.82 %), degradation rates and level of hydrocarbon degraders in the total number of microorganisms, we note that the stages of adaptation of the consortium and intensive degradation phases were in the range of 0-90 days, followed by a slower decomposition phase in the period of 90-210 days. Related to: [http://cer.ihtm.bg.ac.rs/handle/123456789/3299]

The aim of our research was to determine the presence of petroleum pollutants and their vertical migration in the alluvial area of Sava river. Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5192]

One of the largest central heating plants in Belgrade, the Capital of Serbia, is a heating plant in New Belgrade. Being located in the alluvial plains of the Sava River, it represents a potential source of oil pollution for the whole alluvial area. The aim of this work was to investigate compositional changes of oil pollutant during migration in vertical profile of the soil in this area. Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5095]