65 results on '"Inmaculada Riba"'
Search Results
2. Sub-lethal combined effects of illicit drug and decreased pH on marine mussels: A short-time exposure to crack cocaine in CO2 enrichment scenarios
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Denis Modelo de Souza Abessa, Lorena da Silva Souza, Luciane Alves Maranho, Estefanía Bonnail, Daniel Araki Ribeiro, Fernando Sanzi Cortez, Camilo Dias Seabra Pereira, Augusto Cesar, Fabio Hermes Pusceddu, Inmaculada Riba, Ángel DelValls, University of Cádiz, University of Atacama, Santa Cecília University (UNISANTA), Universidade de São Paulo (USP), and Universidade Estadual Paulista (UNESP)
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CO2 enrichment ,Crack cocaine ,biology ,Chemistry ,Ocean acidification ,Mussel ,Aquatic Science ,Contamination ,Oceanography ,biology.organism_classification ,Pollution ,Lipid peroxidation ,chemistry.chemical_compound ,Perna perna ,Environmental chemistry ,Seawater ,Decreased ph ,Biomarkers ,Marine mussel - Abstract
Made available in DSpace on 2022-04-29T08:45:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The increasing CO2-concentrations in the atmosphere promote ocean acidification. Seawater chemistry changes interact with contaminants, such as illicit drugs in the coastal zones. This work evaluates impacts of pH decrease and crack-cocaine exposure on the commercial mussel Perna perna through biomarker responses (lysosomal membrane stability, lipid peroxidation, and DNA strand breaks). The organisms were exposed to different crack-cocaine concentrations (0.5, 5.0, and 50 μg L−1) combined with different pH values (8.3, 8.0, 7.5, 7.0, 6.5, and 6.0) for 96 h. Crack-cocaine in the different acidification scenarios triggered cyto-genotoxicity, which affected the overall health of mussels exposed to cocaine environmentally relevant concentration. This study produced the first data on biomarker responses associated with CO2-induced acidification and illicit drugs (crack-cocaine) in marine organisms. Department of Physico-Chemistry Aquatic Systems Research Group UNESCO/UNITWIN WiCop Faculty of Marine and Environmental Sciences University of Cádiz Centro de Investigaciones Costeras-Universidad de Atacama (CIC-UDA) University of Atacama Department of Ecotoxicology Santa Cecília University (UNISANTA) Department of Marine Sciences Federal University of São Paulo (UNIFESP) Study Center on Pollution and Aquatic Ecotoxicology Paulista State University (UNESP) Study Center on Pollution and Aquatic Ecotoxicology Paulista State University (UNESP) FAPESP: 2018/18456-4 CNPq: 409187/2016-0
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- 2021
3. Comparative effects of seawater acidification on microalgae: Single and multispecies toxicity tests
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Marta Sendra, M. R. De Orte, Inmaculada Riba, Esther Bautista-Chamizo, Junta de Andalucía, European Commission, and Fundação de Amparo à Pesquisa do Estado de São Paulo
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CO2 enrichment ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,Sentinel species ,010501 environmental sciences ,01 natural sciences ,Toxicity Tests ,Microalgae ,Environmental Chemistry ,Seawater ,Flow cytometry ,Ecotoxicity ,14. Life underwater ,Phaeodactylum tricornutum ,Tetraselmis ,Waste Management and Disposal ,Ecosystem ,0105 earth and related environmental sciences ,biology ,pH ,Chemistry ,Multispecies ,Aquatic ecosystem ,Environmental stressor ,Interspecific competition ,Carbon Dioxide ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,Environmental chemistry ,Water Pollutants, Chemical - Abstract
In order to gain knowledge about the potential effects of acidification in aquatic ecosystems, global change research based on microalgae as sentinel species has been often developed. However, these studies are limited to single species tests and there is still a research gap about the behaviour of microalgal communities under this environmental stressor. Thus, the aim of this study was to assess the negative effects of CO under an ecologically realistic scenario. To achieve this objective, two types of toxicity tests were developed; i) single toxicity tests and ii) multispecies toxicity tests, in order to evaluate the effects on each species as well as the interspecific competition. For this purpose, three microalgae species (Tetraselmis chuii, Phaeodactylum tricornutum and Nannochloropsis gaditana) were exposed to two selected pH levels (7.4, 6.0) and a control (pH 8.0). The pH values were choosen for testing different scenarios of CO enrichment including the exchange atmosphere-ocean (pH 7.4) and natural or anthropogenic sources of CO (pH 6.0). The effects on growth, cell viability, oxidative stress, plus inherent cell properties (size, complexity and autofluorescence) were studied using flow cytometry (FCM). Results showed that T. chuii was the most resistant species to CO enrichment with less abrupt changes in terms of cell density, inherent cell properties, oxidative stress and cell viability. Although P. tricornutum was the dominant species in both single and multispecies tests, this species showed the highest decrease in cell density under pH 6.0. Effects of competence were recorded in the multispecies control (pH 8) but this competence was eclipsed by the effects of low pH. The knowledge of biological interactions made by different microalgae species is a useful tool to extrapolate research data from laboratory to the field., The first author thanks the European Union for the doctoral fellowship (2014-0693/001-001-EMJD) and the funding of this work through the Erasmus Mundus Programme MACOMA. M. Sendra thanks Junta de Andalucía for her post-doctoral fellowship and M.R. de Orte thanks FAPESP for the post-doctoral scholarship under the process BEPE 2017/06686-2.
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- 2019
4. Effects of CO2 enrichment on two microalgae species: A toxicity approach using consecutive generations
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Inmaculada Riba, Ángel DelValls, Manoela R. de Orte, Esther Bautista-Chamizo, and Ana R. Borrero-Santiago
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0106 biological sciences ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Climate change ,010501 environmental sciences ,01 natural sciences ,Carbon capture and storage ,Environmental Chemistry ,Marine ecosystem ,Organic matter ,Phaeodactylum tricornutum ,Tetraselmis ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,biology ,010604 marine biology & hydrobiology ,Public Health, Environmental and Occupational Health ,Ocean acidification ,General Medicine ,General Chemistry ,biology.organism_classification ,Pollution ,chemistry ,Environmental chemistry ,Environmental science ,Seawater - Abstract
As a result of the increasing pressure provoked by anthropogenic activities, the world climate is changing and oceans health is in danger. One of the most important factors affecting the marine environment is the well-known process called ocean acidification. Also, there are other natural or anthropogenic processes that produce an enrichment of CO2 in the marine environment (CO2 leakages from Carbon Capture and Storage technologies (CCS), organic matter diagenesis, volcanic vents, etc). Most of the studies related to acidification of the marine environment by enrichment of CO2 have been focused on short-term experiments. To evaluate the effects related to CO2 enrichment, laboratory-scale experiments were performed using the marine microalgae Tetraselmis chuii and Phaeodactylum tricornutum. Three different pH values (two treatments - pH 7.4 and 6.0 - and a control - pH 8.0) were tested on the selected species across four consecutive generations. Seawater was collected and exposed to different scenarios of CO2 enrichment by means of CO2 injection. The results showed different effects depending on the species and the generation used. Effects on T. chuii were shown on cell density, chlorophyll-a and metabolic activity, however, a slight adaptation across generations was found in this last parameter. P. tricornutum was more sensitive to acidification conditions through generations, with practically total growth inhibition in the fourth one. The conclusions obtained in this work are useful to address the potential ecological risk related to acidification by enrichment of CO2 on the marine ecosystem by using consecutive generations of microalgae.
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- 2018
5. Experimental evidence of uncertain future of the keystone ragworm Hediste diversicolor (O.F. Müller, 1776) under climate change conditions
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Adília Pires, Mercedes Conradi, Rosa Freitas, Khurshid Alam Bhuiyan, Inmaculada Riba, Belén Marín Rodríguez, and Ángel Dellvals
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Biogeochemical cycle ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,Physiology ,Climate Change ,Biodiversity ,Climate change ,010501 environmental sciences ,01 natural sciences ,Hediste diversicolor ,Environmental Chemistry ,Animals ,Ecosystem ,Seawater ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Polychaete ,Behavior ,Detritus ,biology ,Ecology ,Polychaeta ,biology.organism_classification ,Pollution ,PH decrease ,Metabolic capacity ,Oxidative stress ,Water Pollutants, Chemical - Abstract
It is currently assumed that climate change related factors pose severe challenges to biodiversity maintenance. This paper assesses the multi-stressor effects of elevated temperature (15 °C as control, 25 °C as elevated) and CO2 levels (pH 8.1 as control, 7.5 and 7.0 representing acidifying conditions) on the physiological (survival and regenerative capacity), behavioral (feeding and burrowing activities), and biochemical changes (metabolic capacity, oxidative status and biotransformation mechanisms) experienced by the keystone polychaete Hediste diversicolor. Temperature rise enlarged the adverse effect of marine acidification on the survival of H. diversicolor, delayed the beginning of the excavation activity, enhancing the negative effects that pH decrease had in the burrowing behavior of this polychaete. Additionally, regardless of the temperature, exposure of H. diversicolor to acidification results in a reduction in the feeding rate. It is the first time that this decreased feeding capacity is found related to seawater acidification in this species. The healing of the wound and the blastemal formation were retarded due to these two climatic factors which hinder the regenerative process of polychaetes. These vital physiological functions of H. diversicolor can be related to the oxidative stress induced by climate change conditions since free radicals overproduced will impair cells functioning, affecting species biochemical and physiological performance, including feeding and tissue regeneration. The present results also demonstrated that although polychaetes' metabolic capacity was enhanced under stress conditions, organisms were still able to increase or maintain their energy reserves. Our findings are of major environmental relevance considering that predicted climate change conditions will affect species vital and ecological and physiological capacities. These can be translated into shrinking not only at the individual and population level but also in microbial and endofaunal diversities, in the detritus processing in estuaries and biogeochemical cycles at the ecosystem level. Thus the conservation of H. diversicolor populations is vital for the normal functioning of estuarine mudflat ecosystems.
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- 2021
6. Will temperature and salinity changes exacerbate the effects of seawater acidification on the marine microalga Phaeodactylum tricornutum?
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Marta Seoane, Esther Bautista-Chamizo, Inmaculada Riba, Ángeles Cid, Marta Sendra, Manoela R. de Orte, European Commission, Junta de Andalucía, Xunta de Galicia, and Fundação de Amparo à Pesquisa do Estado de São Paulo
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Salinity ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,Climate Change ,Temperature salinity diagrams ,010501 environmental sciences ,Ecotoxicology ,01 natural sciences ,Phytoplankton ,Microalgae ,Climate change ,Environmental Chemistry ,Seawater ,Water Pollutants ,Flow cytometry ,Phaeodactylum tricornutum ,Viability assay ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Diatoms ,biology ,pH ,Chemistry ,Temperature ,Marine diatom ,Carbon Dioxide ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,Environmental chemistry ,CO2 ,Environmental Monitoring - Abstract
To evaluate the effects related to the combination of potential future changes in pH, temperature and salinity on microalgae, a laboratory experiment was performed using the marine diatom Phaeodactylum tricornutum. Populations of this species were exposed during 48 h to a three-factor experimental design (3 × 2 × 2) with two artificial pH values (6, 7.4), two levels of temperature (23 °C, 28 °C), two levels of salinity (34 psu, 40 psu) and a control (pH 8, Temp 23 °C, Sal 34 psu). The effects on growth, cell viability, metabolic activity, and inherent cell properties (size, complexity and autofluorescence) of P. tricornutum were studied using flow cytometry. The results showed adverse effects on cultures exposed to pH 6 and high temperature and salinity, being the inherent cell properties the most sensitive response. Also, linked effects of these parameters resulted on cell viability and cell size decrease and an increase of cell autofluorescence. The conclusions obtained from this work are useful to address the potential effects of climate change (in terms of changes on pH, salinity and temperature) in microalgae., The first author is grateful to the Erasmus Mundus Programme for the doctoral fellowship (2014-0693/001-001-EMJD) and also for the founding of this work through the Erasmus Mundus Project MACOMA. M. Sendra thanks Junta de Andalucía (Spain) for her post-doctoral fellowship. M. Seoane thanks Xunta de Galicia (Spain) for her pre-doctoral fellowship. M.R. de Orte thanks FAPESP for the post-doctoral fellowship under the process BEPE 2017/06686-2.
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- 2018
7. Impact of pesticides in karst groundwater. Review of recent trends in Yucatan, Mexico
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Jesús Alfredo Araujo León, Ángel DelValls Casillas, María Inmaculada Riba López, Sudip Datta Banik, and Ángel Rodríguez
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Pollution ,Environmental Engineering ,media_common.quotation_subject ,0208 environmental biotechnology ,Geography, Planning and Development ,Population ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,chemistry.chemical_compound ,Dieldrin ,Environmental protection ,Groundwater pollution ,Environmental Chemistry ,Aldrin ,Water pollution ,education ,0105 earth and related environmental sciences ,Water Science and Technology ,media_common ,education.field_of_study ,geography ,geography.geographical_feature_category ,Karst ,020801 environmental engineering ,chemistry ,Environmental science ,Groundwater - Abstract
The anthropogenic activities in karst aquifer increase the vulnerability for groundwater pollution by organochlorine pesticides (OCP). Recent research have been published about high level of OCP in water, as well as the bioaccumulation of OCP in blood of women with cancer and in breast milk. The use of banned OCP such as DDT, heptachlore, lindane, endosulfan, aldrin, are the main sources of pollution of groundwater in Yucatan, Mexico. In Yucatan, the water pollution is increased by 30% of deforestation, large zones of geological fracturing with high permeability, and high density of sinkholes (cenotes) in the karst soils. Diverse studies indicate Yucatan as a zone with high impact of water pollution (3.2 ppm of endrin, 10.86 ppm of δ-lindane, 5.23 ppm of γ-lindane, 6.53 ppm of α-lindane, 13.61 and 12.54 ppm of heptachlore). 30% of the population drink water from polluted wells and sinkholes. A study showed high levels of OCP in the blood of women with cervical uterine cancer: 7.352 ppm of endosulfan I, 3.695 ppm of aldrin, 2.336 ppm of 4,4′ DDD, 1.434 ppm of heptachlore. Furthermore, recent research show high levels of pesticides in breast milk, indicating 18.436 ppm of heptachlore epoxide and 1.024 and 2.10 ppm of dieldrin. Social conditions such as poverty, low education level, lack of regulation and capacitation in the agricultural sector, as well as environmental vulnerabilities have a high impact on the karst groundwater and the public health. This review showed the evidence of the need of regulation of OCP, the promotion of public health and implementation of agroecological programs.
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- 2018
8. Using a mesocosm approach to evaluate marine benthic assemblage alteration associated with CO2 enrichment in coastal environments
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Inmaculada Riba, A. Cesar, Alice Newton, M.C. Passarelli, and Tomás Ángel DelValls
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0106 biological sciences ,010604 marine biology & hydrobiology ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Sampling (statistics) ,Sediment ,General Medicine ,010501 environmental sciences ,01 natural sciences ,Pollution ,Mesocosm ,Benthic zone ,Abundance (ecology) ,Environmental chemistry ,Environmental science ,Species richness ,Bay ,0105 earth and related environmental sciences - Abstract
The effects of acidification related to the CO2 enrichment in the coastal environments on marine macrobenthic abundance, diversity and richness were analyzed in a medium- term (21 days) using mesocosm experiments. Two sampling sites located in the Bay of Cadiz – SW, Spain were selected and tested at pH values ranged from 7.9 to 6.0 (± 0.1). Moreover, variations in the concentrations of metals in the sediment samples were analyzed at the end of each experiment. The results showed low variation in the concentrations of metals in the sediment among the pH treatments. A significant decrease (p
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- 2018
9. Implications in studies of environmental risk assessments: Does culture medium influence the results of toxicity tests of marine bacteria?
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Inmaculada Riba, Ana R. Borrero-Santiago, and Alejandra Díaz-García
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0301 basic medicine ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,030106 microbiology ,Population ,chemistry.chemical_element ,Metal toxicity ,010501 environmental sciences ,Risk Assessment ,01 natural sciences ,03 medical and health sciences ,Marine bacteriophage ,Nutrient ,Toxicity Tests ,Environmental Chemistry ,Bioassay ,Seawater ,Food science ,education ,0105 earth and related environmental sciences ,Cadmium ,education.field_of_study ,Bacteria ,biology ,Chemistry ,Public Health, Environmental and Occupational Health ,Environmental Exposure ,General Medicine ,General Chemistry ,biology.organism_classification ,Pollution ,Culture Media ,Zinc ,Toxicity ,Water Pollutants, Chemical - Abstract
Two marine bacterial populations (Roseobacter sp. and Pseudomonas litoralis) were exposed to different concentrations of zinc (300, 625, 1250, 2000, 2500 and 5000 mg L−1) and cadmium (75, 250, 340, 500 and 1000 mg L−1) using two culture media (full nutrient Marine Broth 2216 “MB” and 1:10 (vol/vol) dilution with seawater of Marine Broth 2216 “MBSW”), in order to assess population responses depending on the culture medium and also potential adverse effects associated with these two metals. Different responses were found depending on the culture medium (Bacterial abundance (cells·mL−1), growth rates (μ, hours−1), and production of Extracellular Polysaccharides Substances (EPS) (μg glucose·cells−1). Results showed negative effects in both strains after the exposure to Zn treatments. Both strains showed highest metal sensitivity at low concentrations using both culture media. However, different results were found when exposing the bacterial populations to Cd treatments depending on the culture medium. Highest toxicity was observed using MB at low levels of Cd concentrations, whereas MBSW showed toxicity to bacteria at higher concentrations of Cd. Results not only showed adverse effects on Roseobacter sp. and Pseudomonas litoralis associated with the concentration of Zn and Cd, but also confirm that depending on the culture medium results can differ. This work suggests MBSW as an adequate culture medium to study metal toxicity bioassays in order to predict realistic effects on marine bacterial populations.
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- 2018
10. Effects of CO2 enrichment on metal bioavailability and bioaccumulation using Mytilus galloprovincialis
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Inmaculada Riba, Tomás Ángel DelValls, S. Ray, A. Cesar, and M.C. Passarelli
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010504 meteorology & atmospheric sciences ,biology ,Chemistry ,Aquatic ecosystem ,Sediment ,010501 environmental sciences ,Aquatic Science ,Oceanography ,biology.organism_classification ,01 natural sciences ,Pollution ,Mytilus ,Metal bioavailability ,Bioavailability ,Metal ,visual_art ,Bioaccumulation ,Environmental chemistry ,visual_art.visual_art_medium ,Seawater ,0105 earth and related environmental sciences - Abstract
The main aim of this study was to evaluate the bioavailability of metals related to CO2 enrichment on the mussels Mytilus galloprovincialis by metal's bioaccumulation analysis. Two sediment samples were selected and subjected to different pH levels. Concentrations of metals were measured in the overlying seawater and in the whole body of mussels exposed on the 7th, 14th and 21st days. Results showed that the CO2 enrichment in aquatic ecosystems cause significant (p
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- 2018
11. What is the best endpoint for assessing environmental risk associated with acidification caused by CO2 enrichment using mussels?
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Tomás Ángel DelValls, M.C. Passarelli, A. Cesar, and Inmaculada Riba
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0106 biological sciences ,Acute effects ,animal structures ,biology ,010604 marine biology & hydrobiology ,fungi ,Mussel ,010501 environmental sciences ,Aquatic Science ,Oceanography ,biology.organism_classification ,01 natural sciences ,Pollution ,Mytilus ,Environmental risk ,Environmental chemistry ,Environmental science ,Marine ecosystem ,Seawater ,Retention time ,0105 earth and related environmental sciences - Abstract
Carbon capture and storage is a technology that has been widely determined to be one of the best choices for the short-term reduction of atmospheric CO2 emissions. The aim of this study was to analyze the effects of CO2 enrichment in the ocean on the mussel species Mytilus galloprovincialis using three different endpoints: mortality, embryo-larval development, and neutral red retention time assays (NRRT). Acute effects were found to be associated with a pH values of 6.0 while citotoxity effects and embryo-larval development were associated with a pH value of 7.0. The NRRT assay and embryo-larval development can be recommended as good endpoints for assessing the environmental risk associated with acidification by CO2 enrichment because they provide sensitive responses on the effects of changes in seawater pH on mussels in a short period of time. Moreover, this study may support policymakers in finding appropriate solutions for the conservation of marine ecosystems.
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- 2018
12. Comparative evaluation of sea-urchin larval stage sensitivity to ocean acidification
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Tomás Ángel DelValls, A. Cesar, Inmaculada Riba, and M.C. Passarelli
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Environmental Engineering ,010504 meteorology & atmospheric sciences ,Oceans and Seas ,Health, Toxicology and Mutagenesis ,PH reduction ,010501 environmental sciences ,Biology ,01 natural sciences ,chemistry.chemical_compound ,Stress, Physiological ,biology.animal ,Animals ,Environmental Chemistry ,Seawater ,Sea urchin ,Ecosystem ,0105 earth and related environmental sciences ,Lytechinus variegatus ,Larva ,Ecology ,Aquatic ecosystem ,fungi ,Public Health, Environmental and Occupational Health ,Ocean acidification ,General Medicine ,General Chemistry ,Carbon Dioxide ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,chemistry ,Metals ,Spain ,Sea Urchins ,Carbonate ,Acids ,Brazil ,Water Pollutants, Chemical - Abstract
Changes in the marine carbonate system may affect various calcifying organisms. This study is aimed to compare the sensitivity of embryo-larval development of two species of sea urchins (Paracentrutos lividus and Lytechinus variegatus) collected and exposed to samples from different coastal zone (Spain and Brazil) to ocean acidification. The results showed that the larval stages are very sensitive to small changes in the seawater's pH. The larvae from P. lividus species showed to be more sensitive to acidified elutriate sediments than larvae from L. variegatus sea urchin. Furthermore, this study has demonstrated that the CO2 enrichment in aquatic ecosystems cause changes on the mobility of the metals: Zn, Cu, Fe, Al and As, which was presented different behavior among them. Although an increase on the mobility of metals was found, the results using the principal component analysis showed that the pH reduction show the highest correlations with the toxicity and is the main cause of embryo-larval development inhibition. In this comparative study it is demonstrated that both species are able to assess potential effects of the ocean acidification related to CO2 enrichment by both near future scenarios and the risk associated with CO2 leakages in the Carbon Capture and Storage (CCS) process, and the importance of comparative studies in different zones to improve the understanding of the impacts caused by ocean acidification.
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- 2017
13. Effects of a hypothetical escape of CO2 gas from subterranean storage sites on water flea Daphnia magna
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Ángel A. Luque, Alla Khosrovyan, Inmaculada Riba, and Tomás Ángel DelValls
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010504 meteorology & atmospheric sciences ,biology ,Chemistry ,Health, Toxicology and Mutagenesis ,Daphnia magna ,Sediment ,General Medicine ,010501 environmental sciences ,Contamination ,biology.organism_classification ,01 natural sciences ,Pollution ,Water flea ,Water column ,Testing protocols ,Environmental chemistry ,Toxicity ,Environmental Chemistry ,Ecotoxicology ,0105 earth and related environmental sciences - Abstract
The impacts of a hypothetical CO2 gas leak from freshwater sediments on the survival and reproduction of freshwater flea Daphnia magna were analyzed. Another objective was to assess the performance of standard toxicity testing protocols for CO2-induced acidification research in freshwaters. Four pH levels (7.5, 7.0, 6.5, and 6.0) and two sediments with different contamination level were tested. The results revealed that the D. magna are susceptible to a gradual but relatively rapid CO2 enrichment of the water column causing a change from circumneutral to acidic conditions. Standard 48-h immobilization test with D. magna tended to underestimate the toxicity at CO2-induced acidity condition. Dissolved aluminum may be implicated in the toxicity to the parental daphnids exposed. Metal outflux from sediments and behavior in elutriate have been discussed.
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- 2017
14. Bacterial community responses during a possible CO2 leaking from sub-seabed storage in marine polluted sediments
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T. Ángel DelValls, Ana R. Borrero-Santiago, and M. Inmaculada Riba
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Environmental Engineering ,010504 meteorology & atmospheric sciences ,Ph control ,Environmental engineering ,010501 environmental sciences ,Contamination ,01 natural sciences ,Pollution ,Benthic zone ,Environmental chemistry ,Co2 leakage ,Environmental Chemistry ,Environmental science ,Microcosm ,Waste Management and Disposal ,Bioindicator ,Seabed ,0105 earth and related environmental sciences ,Environmental risk assessment - Abstract
Carbon capture and storage (CCS) is a viable option to reduce high concentrations of CO2 and mitigate their negative effects. This option has associated risks such as possible CO2 leakage from the storage sites. So far, negative effects deriving from a CO2 release have been reported for benthic macrofauna in both polluted and nonpolluted sediments. However, bacterial communities has no considered. In this work, risk assessment was carried out in order to evaluate the possible effects in a contaminated area considering bacterial responses (total number of cells, respiring activity, changes in the bacterial community composition and diversity). Four microcosms were placed into an integrated CO2 injection system with a non-pressurized chamber to simulate four different pH treatments (pH control 7.8, 7, 6.5 and 6). Results showed an impact on bacterial communities because of the CO2 treatment. Changes in respiring activity, community composition groups and diversity were found. This study highlights the use of respiring bacteria activity not only as bioindicator for environmental risk assessment and monitoring purposes but also as a bioindicador during a CO2 leakage event or CO2 enrichment process among all the responses studied.
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- 2017
15. A possible CO2 leakage event: Can the marine microbial community be recovered?
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Esther Bautista-Chamizo, Ana R. Borrero-Santiago, Tomás Ángel DelValls, and Inmaculada Riba
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010504 meteorology & atmospheric sciences ,Resistance (ecology) ,Ecology ,010501 environmental sciences ,Aquatic Science ,Biology ,Oceanography ,01 natural sciences ,Pollution ,Marine bacteriophage ,Microbial population biology ,Respiration ,Co2 leakage ,Food science ,Monitoring tool ,0105 earth and related environmental sciences - Abstract
Bacterial communities have been studied to a much lesser degree than macrofauna in the case of a CO2 release. The resistance capacity of marine bacteria is well known, but their possible responses and their ability to recover after a CO2 release has not been investigated. Therefore, this work evaluated the responses of a marine bacterial community after 96h of CO2 exposure under diverse pH treatments (7.8 as control without CO2, 7.0, 6.5, and 6.0) and 24h after CO2 exposure. Results showed that the respiration activity and the diversity of the community were affected in all pH treatments. However, after 24h without CO2 enrichment, the respiration activity and diversity increased, showing a partial recovery. Consequently, bacterial responses have the potential to be used as a monitoring tool for risk assessment related to carbon capture and storage techniques or in any similar CO2 enrichment situations.
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- 2017
16. Simulating CO 2 leakage from sub-seabed storage to determine metal toxicity on marine bacteria
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T. Ángel DelValls, Inmaculada Riba, Alejandra Díaz-García, and Ana R. Borrero-Santiago
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0301 basic medicine ,education.field_of_study ,030106 microbiology ,Population ,Environmental engineering ,Metal toxicity ,010501 environmental sciences ,Aquatic Science ,Biology ,Oceanography ,01 natural sciences ,Pollution ,03 medical and health sciences ,Marine bacteriophage ,Abundance (ecology) ,Environmental chemistry ,Co2 leakage ,Marine ecosystem ,Lack of knowledge ,education ,Seabed ,0105 earth and related environmental sciences - Abstract
CO2 storage in sub-seabed marine geological formations has been proposed as an adequate strategy to mitigate high CO2 concentration from the atmosphere. The lack of knowledge about the potential risks of this technology on marine bacteria population in presence of metals has lead us to perform laboratory-scale experiments in order to evaluate its consequences. Thus, the effects of Zn and Cd were studied under acid conditions on Roseobacter sp. and Pseudomonas litoralis. Bacterial abundance (cells mL− 1), growth rates (μ, h− 1), relative inhibitory effects of CO2 (RICO2), and production of Extracellular Polysaccharides Substances (EPS) (μg Glucose cells− 1) were evaluated. A decreasing exopolysaccharides (EPS) production was found under low pH. Bacterial abundance as well as growth rates showed negative effects. Data obtained in this work are useful to determine the potential effects associated with enrichment of CO2 and metals on the marine ecosystem.
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- 2017
17. CO2 leaking from sub-seabed storage: Responses of two marine bacteria strains
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María Carbú, Ana R. Borrero-Santiago, Inmaculada Riba, and Tomás Ángel DelValls
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Carbon Sequestration ,Geologic Sediments ,010504 meteorology & atmospheric sciences ,Population ,010501 environmental sciences ,Aquatic Science ,Carbon sequestration ,Oceanography ,Global Warming ,01 natural sciences ,Microbiology ,chemistry.chemical_compound ,Marine bacteriophage ,Pseudomonas ,Seawater ,education ,0105 earth and related environmental sciences ,education.field_of_study ,biology ,Sediment ,General Medicine ,Carbon Dioxide ,Hydrogen-Ion Concentration ,Roseobacter ,biology.organism_classification ,Pollution ,chemistry ,Environmental chemistry ,Carbon dioxide ,Water Microbiology ,Water Pollutants, Chemical - Abstract
Carbon capture and storage (CCS) in stable geological locations is one of the options to mitigate the negative effects of global warming produced by the increase in CO2 concentrations in the atmosphere. A CO2 leak is one of the risks associated with this strategy. Marine bacteria attached to the sediment may be affected by an acidification event. Responses of two marine strains (Roseobacter sp. CECT 7117 and Pseudomonas litoralis CECT 7670) were assessed under different scenarios using a range of pH values (7.8, 7, 6.5, 6, and 5.5) to mimic a CO2 leak. A CO2 injection system was used to simulate an escape from a stable sub-seabed. Growth rate (μ), cell number, inhibition of Relative Inhibitory Effect (RI CO2) and inhibited population were analysed as endpoints. P. litoralis showed more sensitivity to high CO2 concentrations than Roseobacter sp. Our results highlight the diversity and resistance in marine bacteria and their capacity to adapt under a stressful CO2 leakage.
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- 2016
18. The influence of ph and waterborne metals on egg fertilization of the blue mussel (Mytilus edulis), the oyster (Crassostrea gigas) and the sea urchin (Paracentrotus lividus)
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Ángel A. Luque, Inmaculada Riba, Bardukh Gabrielyan, T. Angel Del Valls, and Alla Khosrovyan
- Subjects
Male ,0106 biological sciences ,Oyster ,animal structures ,food.ingredient ,Mytilus edulis ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,01 natural sciences ,Paracentrotus lividus ,Human fertilization ,food ,biology.animal ,Paracentrotus ,Animals ,Environmental Chemistry ,Crassostrea ,Sea urchin ,Ovum ,0105 earth and related environmental sciences ,biology ,Ecology ,Chemistry ,010604 marine biology & hydrobiology ,General Medicine ,Mussel ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,Mytilus ,Metals ,Fertilization ,Environmental chemistry ,Female ,Water Pollutants, Chemical ,Blue mussel - Abstract
This study evaluated the combined effect of pH and metals on the egg fertilization process of two estuarine species, the blue mussel (Mytilus edulis), the oyster (Crassostrea gigas) and a marine species, the sea urchin (Paracentrotus lividus). The success of egg fertilization was examined after exposure of gametes to sediment extracts of various degrees of contamination at pH 6.0, 6.5, 7.0, 7.5 and 8.0. At the pH levels from 6.5 to 8.0, the egg fertilization of the different species demonstrated different sensitivity to metal and/or acidic exposure. In all species, the results revealed that egg fertilization was almost completely inhibited at pH 6.0. The egg fertilization of the blue mussel M. edulis was the least sensitive to the exposure while that of the sea urchin P. lividus demonstrated a concentration-dependent response to the pH levels from 6.5 to 8.0. The results of this study revealed that acidity increased the concentration of several metal ions (Cr, Ni, Cu, Zn, Cd, and Pb) but reduced its availability to the organisms, probably related to the reactivity of the ions with most non-metals or to the competition among metals and other waterborne constituents.
- Published
- 2016
19. Simulating CO2 leakages from CCS to determine Zn toxicity using the marine microalgae Pleurochrysis roscoffensis
- Author
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Esther Bautista-Chamizo, Tomás Ángel DelValls, Inmaculada Riba, and Manoela R. de Orte
- Subjects
Environmental Engineering ,010504 meteorology & atmospheric sciences ,Chemistry ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Ocean acidification ,General Medicine ,General Chemistry ,010501 environmental sciences ,01 natural sciences ,Pollution ,Metal bioavailability ,chemistry.chemical_compound ,Environmental chemistry ,Carbon dioxide ,Toxicity ,Environmental Chemistry ,Seawater ,Microalgae growth ,Pleurochrysis roscoffensis ,Optimal growth ,0105 earth and related environmental sciences - Abstract
Due to the current climate change and ocean acidification, a new technology for CO2 mitigation has been proposed, the Carbon dioxide Capture and Storage (CCS). However, there is an ecological risk associated with potential CO2 leakages from the sub-seabed storages sites. To evaluate the effects related to CO2 leakages, laboratory-scales experiments were performed using the marine microalgae Pleurochrysis roscoffensis. Five Zn concentrations were tested at different pHs to study Zn toxicity under acidified conditions. Seawater was collected and submitted to acidification by means of CO2 injection and by HCl addition. Results showed differences between both acidification techniques: while microalgae growth was enhanced by CO2 supply, reaching the optimal growth at pH 6.5 and full inhibition at pH 5.5, HCl acidification growth was inhibited at pH 6.5. Although small concentrations of Zn were positive for P. roscoffensis growth, Zn toxicity increased at lower pHs, and more severely on samples acidified with HCl. The conclusions obtained in this work are useful to address the potential effects on the marine ecosystem related to changes in metal bioavailability during CO2 leakages scenarios.
- Published
- 2016
20. Intraspecific variation in the response of the estuarine European isopod Cyathura carinata (Krøyer, 1847) to ocean acidification
- Author
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Gema Parra, Inmaculada Riba, Mercedes Conradi, Araceli Rodríguez-Romero, Andrea Galotti, J. E. Sánchez-Moyano, Md. Khurshid Alam Bhuiyan, Maria Dolores Basallote, and Ángel DelValls
- Subjects
Environmental Engineering ,010504 meteorology & atmospheric sciences ,media_common.quotation_subject ,Acclimatization ,Population ,010501 environmental sciences ,01 natural sciences ,Population density ,Intraspecific competition ,Environmental Chemistry ,Animals ,Seawater ,education ,Waste Management and Disposal ,0105 earth and related environmental sciences ,media_common ,education.field_of_study ,biology ,Ecology ,Longevity ,Ocean acidification ,Hydrogen-Ion Concentration ,Energy budget ,biology.organism_classification ,Pollution ,Crustacean ,Adaptation, Physiological ,Spain ,Adaptation ,Isopoda - Abstract
In the present study the model isopod, Cyathura carinata were exposed to four pHNIST treatments (control: 7.9; 7.5, 7, 6.5) in order to determine the tolerance and pH threshold value this estuarine species withstand under future acidification scenarios. Seawater acidification significantly affected the lifespan of C. carinata, where population density was remarkably reduced at the lowest pH treatment. The longevity, survivorship and swimming activity (related to the acquisition of energy) of these isopods decreased with decreasing pH. Furthermore, to determine the possible metabolic plasticity of this species, the swimming activity, the Na+/K + -ATPase activity (relevant for osmoregulation process), and the RNA:DNA ratio (an indicator of fitness) were measure from two populations of C. carinata, one inhabiting a stable environment (pHNIST 7.5–8.0) and one inhabiting a fluctuating pCO2 regimes (pH 3.3–8.5) subjected to three pH treatments (7.9, 7.0 and 6.5). The population from high fluctuating pCO2 conditions showed capacity to withstand to pH 6.5, as well as higher longevity and metabolic plasticity, when compared with the population from the habitat with slight pCO2 variation. These results indicate that Cyathura population from stable environments could be vulnerable to ocean acidification because it could trigger detrimental effects on its survival energy budget, and growth. However, ocean acidification has limited effect on the energy budget and survival of C. carinata population from highly variable habitats, suggesting that they are able to cope with the elevated energy demand. The difference showed between populations is likely an indication of genetic differentiation in tolerance to ocean acidification, possibly attributable to local adaptations, which could provide the raw material necessary for adaptation to future conditions. In addition, our results suggest that when assessing marine crustacean responses to changing environments on a global scale, variability in population and metabolic responses need to be considered.
- Published
- 2018
21. Sediment quality assessment in the Guadalquivir River (SW, Spain) using caged Asian clams: A biomarker field approach
- Author
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T. Ángel DelValls, Alessandra Aloise de Seabra, Estefanía Bonnail, and Inmaculada Riba
- Subjects
Geologic Sediments ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,Biological Stress ,010501 environmental sciences ,01 natural sciences ,Water Quality ,Sediment contamination ,Environmental Chemistry ,Animals ,Corbicula fluminea ,Waste Management and Disposal ,Corbicula ,0105 earth and related environmental sciences ,Glutathione Transferase ,Metalloids ,Glutathione Peroxidase ,biology ,Quality assessment ,Sediment ,Contamination ,biology.organism_classification ,Pollution ,Fishery ,Metals ,Spain ,Environmental science ,Biomarker (medicine) ,Biomarkers ,Water Pollutants, Chemical ,Field conditions ,DNA Damage ,Environmental Monitoring - Abstract
This study assesses the sediment quality of the Guadalquivir River watercourse between the Alcala del Rio dam and the city of Seville. The main objective of this work is to address sediment quality in the area using an integrative approach that links sediment contamination and toxicity using the Asiatic clam (Corbicula fluminea) under field conditions. This is the first study conducted in the area that use of a battery of biomarkers from exposure (GST, GPx) to adverse biological effect (DNA and histopathological damage) to identify the contamination adverse effects in a river area affected by a cocktail of different anthropogenic activities (urban, industrial, agricultural, etc.). The sediment quality characterized in the area shows a significant biological stress related to metal(loid)s at station located in Alcala del Rio in the river upper part of the studied area, being this stress toxic when approaching the city of Seville. The sediments located nearby this city showed toxicity by means of positive values in the biomarkers of effects measured in the caged clams and related to contaminants with an industrial and urban discharge origins. These results have shown the useful and strength of the biomarker approach used in this study that combines biomarker responses from exposure to effects and allows identifying the contamination adverse effects by means of using caging individuals of the Asian clam. It has been proved in the different experiments how once the exposure biomarkers reach a maximum value of their system the detoxification ability of the organisms is collapsed and then the biomarkers of effect are measured significantly in the different tissues. The use of field surveys using tolerant specie such as the Asian clam is recommendable to determine sediment quality under an integrative point of view as here reported.
- Published
- 2018
22. Risk Perception and Chronic Exposure to Organochlorine Pesticides in Maya Communities of Mexico
- Author
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Ángel Rodríguez, Patrizia Quattrocchi, M. Inmaculada Riba López, Jorge A. Navarro Alberto, T. Ángel DelValls Casillas, Francisco Javier Solorio Sánchez, and Fernando J. Álvarez Cervera
- Subjects
medicine.medical_specialty ,Poverty ,poverty ,business.industry ,Agrochemical ,Health, Toxicology and Mutagenesis ,Ecological Modeling ,Public health ,Vulnerability ,health ,pesticides ,perception ,Pesticide ,Pollution ,Risk perception ,Geography ,Agriculture ,Environmental protection ,Environmental health ,medicine ,Livestock ,pesticides, risk, perception, health, poverty ,business ,risk - Abstract
In order to analyze risk perception related to the use and handling of organochlorine pesticides (OCP) in agricultural and livestock activities among Maya communities of Yucatan, Mexico, and to study their impact on public health and the environment, we conducted an analytical study applying 274 semi-structured interviews in 11 municipalities in the zone called “Ring of Cenotes.” The harmful effects of agrochemicals on water supplies, soils, air, and human health were considered, including the indoor use of pesticides to preserve harvest products. Recent studies showed high levels of OCP in groundwater. A generalized low risk perception related to human health and the environment due to pesticide use was found. Likewise, social parameters were analyzed, including the educational level, as well as risk factors related to groundwater karst vulnerability. Pesticides that have been banned by international conventions are still in use. The occurrence of some diseases such as cervical cancer, with a ver...
- Published
- 2015
23. Assessing the influence of ocean acidification to marine amphipods: A comparative study
- Author
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Tomás Ángel DelValls, M.C. Passarelli, Inmaculada Riba, F. Serrano-Bernando, and A. Cesar
- Subjects
Geologic Sediments ,Environmental Engineering ,Amphipoda ,010504 meteorology & atmospheric sciences ,Oceans and Seas ,010501 environmental sciences ,Biology ,01 natural sciences ,chemistry.chemical_compound ,Metals, Heavy ,Environmental Chemistry ,Ecotoxicology ,Animals ,Seawater ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Ecology ,Aquatic ecosystem ,Sediment ,Ocean acidification ,Carbon Dioxide ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,chemistry ,Spain ,Environmental chemistry ,Carbon dioxide ,Carbonate ,Brazil ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
CO2 increases in the ocean may occur both by the capacity of CO2 exchanges with its dissolved form between atmosphere and surface seawater as well by CO2 leaks during the carbon capture and storage (CCS) process. The decrease in seawater pH may result in a reduction in the concentration of both hydroxide and carbonate (OH- and CO32-). The main aim of this work is to conduct an ecotoxicology comparative survey using two amphipod species from Europe and Brazil exposed to different acidification (CO2) scenarios. For it, an integrative approach based on the weight of evidence was used for comparative proposes to identify the effects on the amphipods association with the acidification and with the related mobility of metals. The results demonstrate that the Ampelisca brevicornis species is more sensitive to pH reductions than the Hyale youngi species. Furthermore, this study has demonstrated that the CO2 enrichment in aquatic ecosystems would cause changes on the mobility of certain metals (Zn, Cu and As). The results of Principal Component Analysis (PCA) showed that the dissolved Zn in overlying water was strongly correlated with the decrease in the pH and was associated with increased toxicity of the sediment to the exposed organisms, mainly for the A. brevicornis species from Spain. Nevertheless, similar results were found in relation to the mortality of amphipods in low pH values for all sediment tested. Concluding, it is highlighted the importance of comparative studies in different types of environment and improve the understood of the risks associated with the ocean acidification.
- Published
- 2017
24. Simulation of the potential effects of CO 2 leakage from carbon capture and storage activities on the mobilization and speciation of metals
- Author
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Aguasanta Miguel Sarmiento, Inmaculada Riba, T. Ángel DelValls, and Manoela R. de Orte
- Subjects
Geologic Sediments ,Chemistry ,fungi ,Environmental engineering ,Carbon Dioxide ,Hydrogen-Ion Concentration ,Aquatic Science ,Metal analysis ,Contamination ,Oceanography ,Pollution ,Models, Chemical ,Metals, Heavy ,Environmental chemistry ,Co2 leakage ,Sediment contamination ,Computer Simulation ,Ecosystem ,Environmental Pollution ,Geochemical modeling ,Leakage (electronics) - Abstract
One of the main risks associated with carbon capture and storage (CCS) activities is the leakage of the stored CO2, which can result in several effects on the ecosystem. Laboratory-scale experiments were performed to provide data on the possible effects of CO2 leakage from CCS on the mobility of metals previously trapped in sediments. Metal-contaminated sediments were collected and submitted to acidification by means of CO2 injection using different pH treatments. The test lasted 10 days, and samples were collected at the beginning and at the end of the experiment for metal analysis. The results revealed increases in the mobility of metals such as Co, Cu, Fe, Pb and Zn due to pH decreases. Geochemical modeling demonstrated that acidification influenced the speciation of the metals, increasing the concentrations of their free forms. These data suggest the possible sediment contamination consequences of accidental CO2 leakage during CCS activities.
- Published
- 2014
25. Effects on the mobility of metals from acidification caused by possible CO2 leakage from sub-seabed geological formations
- Author
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Inmaculada Riba, Ángel DelValls, Manoela R. de Orte, Maria Dolores Basallote, Araceli Rodríguez-Romero, and Aguasanta Miguel Sarmiento
- Subjects
Environmental Engineering ,Sediment ,Biota ,Contamination ,Geologic Sediments ,Pollution ,Pore water pressure ,chemistry.chemical_compound ,chemistry ,Environmental chemistry ,Carbon dioxide ,Environmental Chemistry ,Environmental science ,Seawater ,Metalloid ,Waste Management and Disposal - Abstract
Carbon dioxide capture and storage (CCS) in submarine geological formations has been proposed as a mitigation measure for the prevention of global warming. However, leakage of CO2 to overlying sediments may occur over time, leading to various effects on ecosystems. Laboratory-scale experiments were performed, involving direct release of carbon dioxide into sediment, inside non-pressurized chambers, in order to provide data on the possible effects of CO2 leakage from geological storage sites on the fate of several metals. Marine sediments from three sites with different levels of contamination were sampled and submitted to acidification by means of CO2 injection. The experiment lasted 10 days and sediment samples were collected at the beginning and end of the experiment and pore water was extracted for metal analysis. The results revealed that mobility of metals from sediment to pore water depends on the site, metal and length of time exposed. Mobilization of the metals Al, Fe, Zn, Co, Pb and Cu increases with acidification, and this response generally increases with time of exposure to CO2 injection. The geochemical model applied suggests that acidification also influences the speciation of metals, transforming metals and metalloids, like As, into species much more toxic to biota. The data obtained from this study will be useful for calculating the potential risk of CCS activities to the marine environment.
- Published
- 2014
26. Levels of persistent organic pollutants in breast milk of Maya women in Yucatan, Mexico
- Author
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M. Inmaculada Riba López, Ángel Rodríguez, B. Anjan Kumar Prusty, Fernando J. Álvarez Cervera, Jesús Alfredo Araujo León, and T. Ángel DelValls Casillas
- Subjects
Chromatography, Gas ,010504 meteorology & atmospheric sciences ,Heptachlor Epoxide ,Heptachlor ,Population ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,Breast milk ,01 natural sciences ,Toxicology ,Dieldrin ,chemistry.chemical_compound ,Soil ,Population Groups ,Hydrocarbons, Chlorinated ,Humans ,Pesticides ,education ,Child ,Groundwater ,Mexico ,Endosulfan ,0105 earth and related environmental sciences ,General Environmental Science ,education.field_of_study ,Milk, Human ,Ecology ,Pesticide Residues ,Agriculture ,General Medicine ,Pollution ,Monitoring program ,chemistry ,Maternal Exposure ,Endrin ,Environmental Pollutants ,Female ,Environmental Monitoring - Abstract
In this study, 24 breast milk samples, obtained from rural Maya women, from municipalities of Yucatan, Mexico, were analyzed for organochlorine pesticide (OCP) residues by gas chromatography. Recent studies have shown that Maya communities have a poor perception about the proper usage and handling of OCP. The karstic soil in this area has a high vulnerability to groundwater pollution by the use of OCP in agriculture and livestock activities. The impact of the ecosystem on human health is much more critical due to the prevailing poverty and a very low educational level of these communities. About 30% of the Maya population consumes water directly from contaminated wells and sinkholes, resulting in a chronic exposure to OCP. The samples served to identify and quantify high levels of OCP residues (18.43 mg/kg of heptachlor epoxide and 1.92 mg/kg of endrin in the metropolitan zone; 2.10 mg/kg of dieldrin, 0.117 mg/kg of endosulfan II, 0.103 mg/kg of heptachlor, 0.178 mg/kg of endrin, and 0.127 mg/kg of endrin aldehyde in the main agricultural zone and on the west coast). The detected levels of OCP residues are a major concern and represent a potential risk to women and children in the region. This could be associated with the high rates of cervical uterine and breast cancer mortality in Yucatan. Thus, regulations on the usage of OCP and their enforcement are necessary, and it is important to establish a yearly monitoring program for OCP residues in breast milk and groundwater, as well as to implement health promotion programs for women in particular and the general population in general.
- Published
- 2016
27. Dredged material characterization and management frameworks: A case study at the port Vilagarcia (NW, Spain)
- Author
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Araceli Rodríguez-Romero, T. Ángel DelValls, Alla Khosrovyan, Inmaculada Riba, Ministerio de Ciencia e Innovación (España), European Commission, and Fundación Universidad Empresa de la provincia de Cádiz
- Subjects
Geologic Sediments ,Engineering ,Embryo, Nonmammalian ,Environmental Engineering ,Amphipoda ,Health, Toxicology and Mutagenesis ,0211 other engineering and technologies ,Weight-of-evidence ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Hazardous Substances ,Paracentrotus lividus ,Toxicity Tests ,Decision tree ,Animals ,Environmental Chemistry ,Ampelisca brevicornis ,Waste Management and Disposal ,Ovum ,0105 earth and related environmental sciences ,021110 strategic, defence & security studies ,biology ,Toxicity ,business.industry ,Environmental engineering ,Sediment ,Dredged material ,biology.organism_classification ,Aliivibrio fischeri ,Pollution ,Port (computer networking) ,Management ,Spain ,Material quality ,Paracentrotus ,business ,Potential toxicity - Abstract
The potential impact of dredged sediment has been assessed at sixteen areas of the high-traffic port of Vilagarcia (Northwest Spanish Atlantic coast). The assessment has been done by three weight-of-evidence tools, which integrated data on sediment characteristics and toxicity responses of Ampelisca brevicornis, Vibrio fischeri and eggs and embryos of Paracentrotus lividus. Two of the tools also represented management options regarding the disposal of dredged material. The comparison of the logic in these tools revealed essential differences in the type and the necessity of bioassays and threshold values for chemical concentrations. However, despite this difference, assessment results and the derived management options coincided in most of the sediments. The potential toxicity of sediments was relatively low especially for eggs and embryos possibly due to different contaminant availability in solid and liquid phases. The importance of a battery of toxicity tests in the dredged material quality assessment has been emphasized to avoid an underestimation of sediment toxicity for solid phase organisms, if only liquid phase responses are considered. The potential false implications, which may result from the application of the third tool, were highlighted. The strengths and weaknesses of the tools were discussed from the dredged material management perspective., This work was partially supported by the financial help of the Spanish Ministry of Science and Innovation through project CTM2012-36476-C02-01/TECNO. The second author thanks the Erasmus Mundus Joint Doctorate program, employer Fundacion Universidad Empresa de la Provincia de Cadiz.
- Published
- 2016
28. Lethal effects on different marine organisms, associated with sediment–seawater acidification deriving from CO2 leakage
- Author
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Maria Dolores Basallote, Ángel DelValls, Araceli Rodríguez-Romero, Julián Blasco, and Inmaculada Riba
- Subjects
Geologic Sediments ,Health, Toxicology and Mutagenesis ,Population ,Biological Availability ,Ruditapes ,Carbon capture and storage (CCS) ,Biology ,Toxicity Tests ,Animals ,Environmental Chemistry ,Bioassay ,Ecotoxicology ,Seawater ,Sediment elutriates ,education ,Toxicity test ,education.field_of_study ,pH ,Ecology ,Sediment ,Polychaeta ,General Medicine ,Carbon Dioxide ,Hydrogen-Ion Concentration ,Bivalvia ,biology.organism_classification ,Pollution ,Sea Bream ,Metals ,Spain ,Larva ,Environmental chemistry ,Hediste diversicolor ,Biological Assay ,Water Pollutants, Chemical - Abstract
CO2 leakages during carbon capture and storage in sub-seabed geological structures could produce potential impacts on the marine environment. To study lethal effects on marine organisms attributable to CO2 seawater acidification, a bubbling CO2 system was designed enabling a battery of different tests to be conducted, under laboratory conditions, employing various pH treatments (8. 0, 7. 5, 7. 0, 6. 5, 6. 0, and 5. 5). Assays were performed of three exposure routes (seawater, whole sediment, and sediment elutriate). Individuals of the clam (Ruditapes philippinarum) and early-life stages of the gilthead seabream, Sparus aurata, were exposed for 10 days and 72 h, respectively, to acidified clean seawater. S. aurata larvae were also exposed to acidified elutriate samples, and polychaete organisms of the specie Hediste diversicolor and clams R. philippinarum were also exposed for 10 days to estuarine whole sediment. In the fish larvae elutriate test, 100 % mortality was recorded at pH 6. 0, after 48 h of exposure. Similar results were obtained in the clam sediment exposure test. In the other organisms, significant mortality (p < 0.05) was observed at pH values lower than 6. 0. Very high lethal effects (calculating L[H+]50, defined as the H+ concentration that causes lethal effects in 50 % of the population exposed) were detected in association with the lowest pH treatment for all the species. The implication of these results is that a severe decrease of seawater pH would cause high mortality in marine organisms of several different kinds and life stages. The study addresses the potential risks incurred due to CO2 leakages in marine environments. © 2012 Springer-Verlag., The work described was supported partially by grants from the Spanish Ministry of Science and Innovation (CTM2008-06344-C03-02/ TECNO, CTM2008-06344-C03-03/TECNO, and CTM2011-28437-C02-02) and by grant P08-556 RNM 3924 from the Regional Government of Andalusia (Junta de Andalucía).
- Published
- 2012
29. Bioaccumulation and Effects of Metals Bound to Sediments Collected from Gulf of Cádiz (SW Spain) Using the Polychaete Arenicola marina
- Author
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Julián Blasco, Judit Kalman, Inmaculada Riba, and Ángel DelValls
- Subjects
Geologic Sediments ,Health, Toxicology and Mutagenesis ,Biological Availability ,Toxicology ,Contamination ,Gulf of Cadiz ,Plychaeta ,Metals, Heavy ,Metalloproteins ,Geologic sediments ,Animals ,Ecotoxicology ,Arenicola marina ,Polychaete ,Biological markers ,biology ,Chemistry ,Ecology ,Polychaeta ,General Medicine ,biology.organism_classification ,Pollution ,Metal bioavailability ,Rate of increase ,Bioavailability ,Spain ,Environmental chemistry ,Bioaccumulation ,Multivariate Analysis ,Arenicola ,Biomarkers ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
A short-term whole-sediment test using the polychaete Arenicola marina was conducted under laboratory conditions to assess the bioavailability of metals bound to sediments collected from 12 sites of the Gulf of Cádiz. To achieve this objective, the rate of increase of metal bioaccumulation and the induction of a typical biomarker, metallothioneinlike proteins (MTLPs) were determined. Results of the multivariate analysis showed associated metal-rich sediments, increased rate of Cu and Zn accumulations, but lower toxicity with an increased MTLP induction, whereas sedimentary Ni and Co concentrations were related to higher toxicity to lugworms, although it might be caused by other contaminants present in these sediments. The linear kinetic approach was shown to be valid in certain circumstances, but more validation studies of this parameter are required before it can be recommended for use in evaluating metal bioavailability in sediments., This work was supported by the project “Interreg IIIA Cooperación Transfronteriza España-Portugal, FEDER-EU” (SP3.E101/03). J. K. thanks the i3p program of the Spanish National Research Council for funding her research fellowship.
- Published
- 2011
30. Influence of sediment acidification on the bioaccumulation of metals in Ruditapes philippinarum
- Author
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Julián Blasco, Carlos Vale, Inmaculada Riba López, and Judit Kalman
- Subjects
Geologic Sediments ,Health, Toxicology and Mutagenesis ,chemistry.chemical_element ,Ruditapes ,Sediment quality ,Animals ,Environmental Chemistry ,Ecotoxicology ,Organic matter ,Water pollution ,chemistry.chemical_classification ,Cadmium ,biology ,pH ,Clams ,Sediment ,General Medicine ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,Bivalvia ,chemistry ,Metals ,Environmental chemistry ,Bioaccumulation ,Seawater ,Carbon dioxide capture ,Estuaries ,Acids ,Water Pollutants, Chemical - Abstract
10 páginas, 3 figuras, 1 tabla., Background, aim and scope: The influence of pH (range 6.5–8.5) on the uptake of Zn, Cd, Pb, Cu, Ni, Cr, Hg, and As by juveniles of the clam Ruditapes philippinarum was examined in order to understand whether variation in sediment pH has significant repercussions on metal bioaccumulation. Materials and methods: Clams were exposed to sediments collected in three locations in the Gulf of Cadiz (Huelva, Guadalquivir and Bay of Cadiz) and to contaminated particles derived from an accidental mining spill in Spain. Results: With a notable exception of metal Cd, the concentration of metals within clams significantly increased (p, The described work was partially supported by grants funded by the Spanish Ministry of Science and Innovation (CTM2008-06344-C03-02/TECNO and CTM2008-06344-C03-03/TECNO) and by grant P08-RNM-3924 funded by ‘Junta de Andalucía’. Dra. Inmaculada Riba (Ramon y Cajal contract) thanks the Spanish Science and Innovation program ‘Jose Castillejo’ for supporting her stay at IPIMAR. Judit Kalman also thanks the I3P program for funding her research at ICMAN/CSIC.
- Published
- 2010
31. Influence of Salinity in the Bioavailability of Zn in Sediments of the Gulf of Cádiz (Spain)
- Author
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Alejandra Maz-Courrau, María Luisa González de Canales, E. García-Luque, Tomás Ángel DelValls, and Inmaculada Riba
- Subjects
Gill ,Environmental Engineering ,Bioavailability ,Toxicity ,biology ,Chemistry ,Ecological Modeling ,Sediment ,Ruditapes ,biology.organism_classification ,Pollution ,Salinity ,Zinc ,Oceanography ,Ruditapes philippinarum ,Bioaccumulation ,Environmental chemistry ,Environmental Chemistry ,Bioassay ,Gulf of Cádiz ,Water Science and Technology - Abstract
This study assesses the effect of salinity in bioavailability and toxicity of Zn by means of laboratory bioassays by observing contamination in both sediment and water, accumulation of Zn in biological tissues, and histopathological damage in the gills and guts tissues of Ruditapes philippinarum clams, which were exposed to different types of sediments from the Gulf of Cádiz (SW Spain) as well as two dilutions of toxic mud coming from an accidental mining spill. With this objective, the coefficients of distribution (K D) for Zn between overlying water and sediments were calculated, the histopathological frequencies in the tissues of the gills and guts of clams were determined, and the biota-sediment bioaccumulation factors as well as the bioaccumulation factors were quantified in the different stations. Results showed that the greatest histopathological damages appeared when the salinity values decreased. Statistical results showed that salinity was inversely correlated with histopathological damage (p
- Published
- 2010
32. A weight of evidence approach for quality assessment of sediments impacted by an oil spill: The role of a set of biomarkers as a line of evidence
- Author
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Inmaculada Riba, T. Ángel DelValls, Carmen Morales-Caselles, Unidad Asociada de Calidad Ambiental y Patologı´a (CSIC & UCA) instituto de Ciencias Marinas de Andalucı´a (ICMAN-CSIC), Facultad de Ciencias del Mar y Ambientales, and Universidad de Cádiz (UCA)
- Subjects
Geologic Sediments ,010504 meteorology & atmospheric sciences ,Chemical Hazard Release ,010501 environmental sciences ,Aquatic Science ,Oceanography ,01 natural sciences ,Benthos ,Toxicity Tests, Acute ,Water Pollution, Chemical ,Animals ,Ecosystem ,Set (psychology) ,ComputingMilieux_MISCELLANEOUS ,Environmental quality ,0105 earth and related environmental sciences ,Pollutant ,business.industry ,Environmental resource management ,Life Sciences ,Sediment ,General Medicine ,Invertebrates ,Pollution ,6. Clean water ,Petroleum ,Spain ,13. Climate action ,Benthic zone ,Environmental chemistry ,Environmental science ,Water quality ,business ,Biomarkers ,Environmental Monitoring - Abstract
In an attempt to incorporate both line of evidence (LOE) and classical weight of evidence (WOE) approaches for the assessment of sediment quality, a set of biomarkers were analyzed in target tissues of two invertebrate species after 28 days of exposure to sediments impacted by oil (derived from the tanker Prestige (2002)). The integration of biomarkers with sediment contamination, acute toxicity and benthic alteration parameters provides an "early warning" tool which not only indicates the environmental quality of an area, but also constitutes an advisory tool for potential ecological risks. The selected biomarkers provide information about the first biological responses due to the presence of contaminants in the environment providing predictable reports about further effects to the ecosystem. The present study demonstrates that the use of a set of biomarkers as part of a WOE approach designed to assess contaminated sediments contributes added value to the classical LOE and allows characterization of the environmental status of the studied area in a more precise and accurate way.
- Published
- 2009
33. Sublethal responses in caged organisms exposed to sediments affected by oil spills
- Author
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Carmen Sarasquete, T. Ángel DelValls, M. Laura Martín-Díaz, Inmaculada Riba, and Carmen Morales-Caselles
- Subjects
Gills ,Geologic Sediments ,Environmental Engineering ,Antioxidant ,Brachyura ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,Glutathione reductase ,Histopathology ,Ruditapes ,Antioxidants ,Disasters ,Contaminants ,Cytochrome P-450 CYP1A1 ,Water Pollution, Chemical ,medicine ,Animals ,Environmental Chemistry ,Ecotoxicology ,Bioassay ,Invertebrate ,Carcinus maenas ,Polycyclic Aromatic Hydrocarbons ,Glutathione Transferase ,chemistry.chemical_classification ,Glutathione Peroxidase ,Toxicity ,biology ,Glutathione peroxidase ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,biology.organism_classification ,Polychlorinated Biphenyls ,Pollution ,Bivalvia ,Glutathione Reductase ,Petroleum ,chemistry ,Metals ,Environmental chemistry ,Phase II Detoxification ,Factor Analysis, Statistical ,Digestive System ,Water Pollutants, Chemical ,Biomarkers - Abstract
7 páginas, 5 figuras, 2 tablas., This study was performed to determine sublethal responses of two invertebrate species by using field deployments in areas affected by oil spills, which are acute in the Galician Coast (NNW, Spain) and chronic in the Bay of Algeciras (SSW, Spain). The organisms employed were the crab Carcinus maenas and the clam Ruditapes philippinarum, and during 28 days the animals were exposed to contaminated sediments in cages under field conditions. Different biomarkers of exposure were determined after a 28-day period exposure: ethoxyresorufin O-deethylase (EROD), phase I detoxification enzyme, glutathione-S-transferase (GST) phase II detoxification enzyme but also implicated in oxidative stress events, glutathione peroxidase (GPX) and glutathione reductase (GR), both antioxidant enzymes. In addition, histopathological effects in target tissues of the deployed organisms were evaluated. Biomarker measurements were linked with the concentration of chemicals in the sediments in order to elucidate the type, source and bioavailability of contaminants that produce adverse effects in the bioindicator species. Results obtained in this study have shown how the application of the selected battery of biomarkers under field bioassays allows for the identification of alternative sources of stress that are not observable in laboratory experiments., The work described was partly supported by the projects VEM2003-20563/INTER, and CTM2005-07282-C03-01/TECNO financed by the Spanish Education and Science Ministry and by CIS funded by the Ministry of Environment. Carmen Morales-Caselles thanks the Ministry of Education and Science for funding her research fellowship (FPU). We are grateful for the support and help of the members of the CIS and the ICMAN-CSIC.
- Published
- 2008
34. Using a classical weight-of-evidence approach for 4-years' monitoring of the impact of an accidental oil spill on sediment quality
- Author
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T. Ángel DelValls, Inmaculada Riba, Carmen Sarasquete, and Carmen Morales-Caselles
- Subjects
Pollution ,Geologic Sediments ,media_common.quotation_subject ,Sediment quality triad ,PAHs ,Contamination ,Crustacea ,Environmental monitoring ,Water Pollution, Chemical ,Animals ,Amphipoda ,Quality (business) ,Polycyclic Aromatic Hydrocarbons ,lcsh:Environmental sciences ,Environmental quality ,General Environmental Science ,media_common ,WOE ,lcsh:GE1-350 ,Hydrology ,Toxicity ,Sediment ,Polychaeta ,Biodiversity ,Metals ,Mollusca ,Spain ,Benthic zone ,Multivariate Analysis ,Environmental science ,Oils ,Environmental Monitoring - Abstract
10 páginas, 7 figuras, 5 tablas., In the present report, the successful application of a Weight of evidence approach (WOE) to sediment quality assessment during a four year impact period following an oil spill is discussed. The study assesses the sediment quality on the Galician Coast (NW Spain) which was impacted by an accidental spill associated with the sinking of the tanker Prestige (2002). The assessment is based on three lines of evidence: physicochemical characterization of the sediments; determination of acute toxicity by conducting sediment toxicity tests and benthic alteration including taxonomic identifications along with community descriptive statistics. The data obtained were integrated using a WOE approach by means of two different methodologies: multivariate analysis and ANOVA-based pie charts. Results confirm that PAHs related to the Prestige oil spill are the main contaminant associated with biological effects in the area which has since recovered from the initial acute impact. Also, the WOE allowed the identification of metal contamination not previously described in the area responsible for toxicity in sediments analyzed. In addition, the methodology proposed to link the 3 lines of evidence results shows the use for the first time of an objective indice based on factor analysis which allows pollution of the sediments studied to be qualitatively and quantitatively evaluated while demonstrating the WOE approach to be recommendable in monitoring environmental quality., The work described was partly supported by the projects VEM2003-20563/INTER, and CTM2005-07282-C03-01/TECNO financed by the Spanish Education and Science Ministry and by the CIS funded by the Ministry of Environment. Carmen Morales-Caselles thanks the Ministry of Education and Science for funding her research fellowship (FPU).
- Published
- 2008
35. Sediment contamination, bioavailability and toxicity of sediments affected by an acute oil spill: Four years after the sinking of the tanker Prestige (2002)
- Author
-
Cristina Micaelo, Carmen Morales-Caselles, Carlos Vale, Ana Maria da Costa Ferreira, Judit Kalman, T.A. DelValls, and Inmaculada Riba
- Subjects
Pollution ,Geologic Sediments ,Time Factors ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Disasters ,Lugworm ,Animals ,Environmental Chemistry ,Amphipoda ,Toxicity Tests, Chronic ,media_common ,Persistent organic pollutant ,biology ,Ecology ,Public Health, Environmental and Occupational Health ,Sediment ,Polychaeta ,Biota ,General Medicine ,General Chemistry ,biology.organism_classification ,Acute toxicity ,Petroleum ,Spain ,Bioaccumulation ,Environmental chemistry ,Environmental science ,Biological Assay ,Bay ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Sediment contamination and three bioassays were used to determine the sediment quality four years after an oil spill (Prestige, 2002): the Microtox® test, a 10-day bioassay using the amphipod Ampelisca brevicornis, and a polychaete 10-day toxicity test with the lugworm Arenicola marina. In addition, bioaccumulation of PAHs was examined in the polychaete after 10 days of exposure. The results obtained from the toxicity tests and bioaccumulation analyses were statistically compared to the sediment chemical data, in order to assess the bioavailability of the contaminants, their effects, and their relationship with the oil spill. The sediments studied were from two areas of the Galician Coast (NW Spain): the Bay of Corme-Laxe and the Cies Island, located in the Atlantic Island National Park. The results point to a decrease in contamination with respect to previous studies and to the disappearance of the acute toxicity four years after the oil spill. However an important bioaccumulation of PAHs was detected in the organisms exposed to sediments from Corme-Laxe, suggesting that despite the recovery of the environmental quality of the area, effects in the biota might be occurring.
- Published
- 2008
36. Comparing sediment quality in Spanish littoral areas affected by acute (Prestige, 2002) and chronic (Bay of Algeciras) oil spills
- Author
-
Inmaculada Riba, Judit Kalman, Tomás Ángel DelValls, and Carmen Morales-Caselles
- Subjects
Geologic Sediments ,Amphipoda ,Health, Toxicology and Mutagenesis ,Industrial Waste ,Toxicology ,Disasters ,PAHs ,Contamination ,Metals, Heavy ,Toxicity Tests ,Littoral zone ,Animals ,Ecotoxicology ,Seawater ,Polycyclic Aromatic Hydrocarbons ,Water pollution ,Ships ,Vibrio ,Hydrology ,Toxicity ,biology ,Microtox® ,Prestige ,Water Pollution ,Sediment ,General Medicine ,Amphipods ,biology.organism_classification ,Polychlorinated Biphenyls ,Pollution ,Fishery ,Environmental science ,Bay ,Fuel Oils ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
8 páginas, 4 figuras, 2 tablas., The quality of sediments collected from two areas of the Spanish coast affected by different sources of contaminants has been compared in this study. The areas studied are the coast of Galicia affected by the oil spill from the tanker Prestige (November 2002) and the Gulf of Cádiz which suffers continuous inputs of contaminants from industries located in the area and from oil spills. Contamination by several chemicals (metals, PCBs and PAHs) that bind to sediments was analyzed, and two toxicity tests (Microtox® and amphipod 10-day bioassay) were conducted. PAHs were identified as the compounds responsible for the toxic effects. Results show differences between an acute impact related to the sinking of the tanker Prestige and the chronic impact associated with continuous oil spills associated with the maritime and industrial activities in the Bay of Algeciras, this being the most polluted part of the two coastal areas studied in this work., The work described was partly supported by a Grant funded by the Ministry of Education and Science VEM2003-20563 and by CIS funded by the Ministry of Environment. Carmen Morales-Caselles thanks the Ministry of Education and Science for funding her research fellowship (FPU).
- Published
- 2007
37. Carbon Capture and Storage (CCS): Risk assessment focused on marine bacteria
- Author
-
Ana R. Borrero-Santiago, Tomás Ángel DelValls, and Inmaculada Riba
- Subjects
Aquatic Organisms ,Carbon Sequestration ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,Climate Change ,Oceans and Seas ,Climate change ,Context (language use) ,010501 environmental sciences ,01 natural sciences ,Risk Assessment ,Marine bacteriophage ,Exponential growth ,Toxicity Tests ,Carbon capture and storage ,Seawater ,0105 earth and related environmental sciences ,Bacteria ,Ecology ,Public Health, Environmental and Occupational Health ,Environmental engineering ,Ocean acidification ,General Medicine ,Carbon Dioxide ,Hydrogen-Ion Concentration ,Pollution ,Environmental science ,Risk assessment ,Water Pollutants, Chemical - Abstract
Carbon capture and storage (CCS) is one of the options to mitigate the negative effects of the climate change. However, this strategy may have associated some risks such as CO2 leakages due to an escape from the reservoir. In this context, marine bacteria have been underestimated. In order to figure out the gaps and the lack of knowledge, this work summarizes different studies related to the potential effects on the marine bacteria associated with an acidification caused by a CO2 leak from CSS. An improved integrated model for risk assessment is suggested as a tool based on the rapid responses of bacterial community. Moreover, this contribution proposes a strategy for laboratory protocols using Pseudomona stanieri (CECT7202) as a case of study and analyzes the response of the strain under different CO2 conditions. Results showed significant differences (p≤0.05) under six diluted enriched medium and differences about the days in the exponential growth phase. Dilution 1:10 (Marine Broth 2216 with seawater) was selected as an appropriate growth medium for CO2 toxicity test in batch cultures. This work provide an essential and a complete tool to understand and develop a management strategy to improve future works related to possible effects produced by potential CO2 leaks.
- Published
- 2015
38. Assessment of metal contamination, bioavailability, toxicity and bioaccumulation in extreme metallic environments (Iberian Pyrite Belt) using Corbicula fluminea
- Author
-
Inmaculada Riba, Estefanía Bonnail, José Miguel Nieto, Aguasanta Miguel Sarmiento, and Tomás Ángel DelValls
- Subjects
Environmental Engineering ,010504 meteorology & atmospheric sciences ,Metal toxicity ,010501 environmental sciences ,01 natural sciences ,Mining ,Biomonitoring ,Environmental Chemistry ,Animals ,Corbicula fluminea ,Waste Management and Disposal ,Corbicula ,0105 earth and related environmental sciences ,Iberian Pyrite Belt ,biology ,Environmental engineering ,Contamination ,Acid mine drainage ,biology.organism_classification ,Pollution ,Metals ,Spain ,Bioaccumulation ,Environmental science ,Water quality ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
The Iberian Pyrite Belt (SW Iberian Peninsula) has intense mining activity. Currently, its fluvial networks receive extremely acid lixiviate residue discharges that are rich in sulphates and metals in solution (acid mine drainage, AMD) from abandoned mines. In the current study, the sediment and water quality were analysed in three different areas of the Odiel River to assess the risk associated with the metal content and its speciation and bioavailability. Furthermore, sediment contact bioassays were performed using the freshwater clam Corbicula fluminea to determine its adequacy as a biomonitoring tool in relation to theoretical risk indexes and regulatory thresholds. Reburial activity and mortality were used as the toxic responses of clams when exposed to contaminated sediment. The results showed coherence between the water and sediment chemical contamination for most of the metals. The reburial activity was correlated with the metal toxicity, but no clam mortality was registered. The bioaccumulation of the studied metals in the clam did not have a significant correlation with the bioavailable fraction of the metal content in the environment, which could be related to a potential different speciation in this singular environment. The bioaccumulation responses were negative for As, Cd and Zn in highly contaminated environments and were characterized as severe, considerable and low potential environmental risks, respectively. The results show that C. fluminea is a good biomonitor of Cu and Pb.
- Published
- 2015
39. Alterations in the macrobenthic fauna from Guadarranque River (Southern Spain) associated with sediment-seawater acidification deriving from CO2 leakage
- Author
-
Mercedes Conradi, Inmaculada Riba, V. Almagro-Pastor, and Tomás Ángel DelValls
- Subjects
Geologic Sediments ,Fauna ,Alkalinity ,Aquatic Science ,Oceanography ,Mesocosm ,Rivers ,Abundance (ecology) ,Animals ,Seawater ,biology ,Ecology ,Sediment ,Ocean acidification ,Polychaeta ,Carbon Dioxide ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,Spain ,Environmental chemistry ,Hediste diversicolor ,Environmental science ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Nowadays, Carbon Storage in Sub-Seabed Geological Structures (CS-SSGS) is having much interest. Nonetheless, these technologies are still under development, especially the leakage of the stored CO2 and the consequent acidification of the environment. Therefore, the goal of this study is to test the impact of CO2-induced acidification on a macrobenthic community due to leakages from CS-SSGS using a mesocosm-based experiment. Results confirmed the significant correlation between the abundance of the species and the pH (positively), and the alkalinity (negatively). Additionally, the BIOENV analysis showed that the majority of the variability in the abundance of the total species was explained for the alkalinity. The correlation analysis showed differential vulnerabilities of different species, especially Cyathura carinata and a non-calcifier species as Hediste diversicolor. Nevertheless, these results showed the importance of taking into account the indirect effect associated with acidification processes, as metal release from sediment.
- Published
- 2014
40. Heavy metal bioavailability and effects: II. Histopathology–bioaccumulation relationships caused by mining activities in the Gulf of Cádiz (SW, Spain)
- Author
-
Inmaculada Riba, T. Ángel DelValls, Natalia Jiménez-Tenorio, M.L. González de Canales, and Julián Blasco
- Subjects
Gills ,medicine.medical_specialty ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Biological Availability ,Mining ,Environmental protection ,Metals, Heavy ,medicine ,Animals ,Environmental Chemistry ,Ecotoxicology ,Tissue Distribution ,Water pollution ,Scrobicularia plana ,geography ,geography.geographical_feature_category ,biology ,Fishes ,Public Health, Environmental and Occupational Health ,Estuary ,General Medicine ,General Chemistry ,biology.organism_classification ,Pollution ,Mollusca ,Spain ,Environmental chemistry ,Bioaccumulation ,Microscopy, Electron, Scanning ,Environmental science ,Crassostrea ,Histopathology ,Digestive System ,Bay ,Water Pollutants, Chemical - Abstract
The relationship between bioaccumulation of heavy metals (Zn, Cd, Pb and Cu) and histological lesions in different tissues of organisms is assessed in three different areas located in the southwest of Spain in the Gulf of Cadiz (Ria of Huelva, Guadalquivir estuary and Bay of Cadiz) affected and non-affected by mining activities. Data included in these relationships were obtained along the years 2000 and 2001 to address the impact of the Aznalcollar mining spill on the Guadalquivir estuary. The bioaccumulation and the histological lesions measured in this seasonal study in the Gudalquivir estuary were linked to derive tissue quality guidelines (TQGs) by means of a multivariate analysis approach (MAA). Sediments collected in the same areas of study were used to expose organisms during the survey carried out in autumn 2001 and to address the relationship between bioaccumulation and histological lesions under laboratory conditions and related to chemicals bound to sediments. Lesions show that the organisms collected in the ria of Huelva and exposed to their sediments were severe, intermediate in the Guadalquivir estuary and absent in the Bay of Cadiz. Results show that the Guadalquivir estuary trends to recover its initial status quo previous to the mining spill. The link between chemical concentration and the lesions measured in the same tissues using MAA permits to derive tissue quality guidelines for two organisms, oysters (Crassostrea angulata) and clams (Scrobicularia plana) collected in the Guadalquivir estuary and associated with the heavy metals from the mining spill (Zn and Cd). The TQG values expressed as concentrations (mg kg−1––dry weight) not associated with biological effects are for oysters, Zn, 8603, Cd, 3.42; and for clams Zn, 800, Cd, 2.6.
- Published
- 2005
41. Heavy metal bioavailability and effects: I. Bioaccumulation caused by mining activities in the Gulf of Cádiz (SW, Spain)
- Author
-
Julián Blasco, Natalia Jiménez-Tenorio, Inmaculada Riba, and T. Ángel DelValls
- Subjects
Oyster ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Biological Availability ,Models, Biological ,Mining ,Metals, Heavy ,biology.animal ,medicine ,Animals ,Environmental Chemistry ,Ecotoxicology ,Tissue Distribution ,Water pollution ,geography ,geography.geographical_feature_category ,biology ,Fishes ,Public Health, Environmental and Occupational Health ,Sediment ,Estuary ,General Medicine ,General Chemistry ,Seasonality ,medicine.disease ,Pollution ,Oceanography ,Mollusca ,Spain ,Bioaccumulation ,Environmental science ,Bay ,Water Pollutants, Chemical - Abstract
The bioaccumulation of six metals (Fe, Mn, Zn, Cd, Pb and Cu) was studied as part of the monitoring of the Aznalcóllar mining spill (April 1998) on the Guadalquivir estuary and in other estuaries located in the Gulf of Cádiz. Fish, clams and oysters were collected during different seasonal periods along the years 2000 and 2001 in the Guadalquivir estuary to determine the bioaccumulation of the metals originated by the mining spill. Results were compared to the bioaccumulation of the same metals in fish and clams exposed in the laboratory to sediments collected in the same areas during autumn 2001. The bioaccumulation of these metals was compared to the concentration of metals measured in tissues of same taxas collected in the areas of the ria of Huelva and the Bay of Cádiz. Results show that the bioaccumulation of Zn and Cd in the organisms sampled in the Guadalquivir estuary was associated with the enrichment of these metals in the estuary from the mining spill and decreased along the time reaching the lowest values in autumn 2001. The metal Cu show different trends that are associated with other sources of contamination than the spill and related to the transport of this metal from Huelva to Guadalquivir estuary and/or to the use of this metal as plaguicide in the rice fields located in the area. The comparison between bioaccumulation results under field and laboratory conditions obtained in the different areas of study shows that these data can be used to discriminate between acute and chronic impacts associated with mining activities.
- Published
- 2005
42. Sediment quality in littoral regions of the Gulf of Cádiz: a triad approach to address the influence of mining activities
- Author
-
Jesús M. Forja, T. Ángel DelValls, Inmaculada Riba, and Abelardo Gómez-Parra
- Subjects
Pollution ,Geologic Sediments ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Biological Availability ,Toxicology ,Mining ,Metals, Heavy ,Littoral zone ,Polycyclic Aromatic Hydrocarbons ,Water pollution ,media_common ,Hydrology ,geography ,geography.geographical_feature_category ,Community structure ,Sediment ,Estuary ,General Medicine ,Spain ,Benthic zone ,Bioaccumulation ,Environmental science ,Environmental Pollution ,Environmental Monitoring - Abstract
To evaluate sediment quality in different areas in the Gulf of Cádiz affected by mining activities, an integrative approach to assessment was used: the Sediment Quality Triad (SQT). Sediment samples were collected at six stations and subjected to replicated sediment acute and chronic toxicity tests, and comprehensive sediment chemistry analyses. Organisms collected synoptically at the same stations were analyzed for histopathological lesions and chemical concentrations in their tissues to determine 'in situ' alteration and bioavailability, in place of benthic community structure. The results obtained for each component were linked to the SQT using different methodologies of interpretation and expression of the integrated approach: (a) multivariate analysis, (b) significant statistical differences compared to the reference stations, (c) ANOVA-based pie charts, and (d) classical methods using triangles and the SQT index of pollution. SQT results indicated that the highest pollution was mainly associated with metals from mining activities and polyaromatic hydrocarbons in the Ría of Huelva. The use of histopathological measures and bioaccumulation of metals, improved the characterization of 'gray areas' of pollution and in the determination of the bioavailability of metals.
- Published
- 2004
43. Monitoring the Impact of the Aznalcóllar Mining Spill on Recent Sediments from the Guadalquivir Estuary, Southwest Spain
- Author
-
Tomás Ángel DelValls, Abelardo Gómez-Parra, Inmaculada Riba, and Jesús M. Forja
- Subjects
Pollution ,Geologic Sediments ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Industrial Waste ,chemistry.chemical_element ,Toxicology ,Mining ,Xenobiotics ,Metals, Heavy ,Accidents, Occupational ,Ecotoxicology ,Ecosystem ,media_common ,Cadmium ,geography ,geography.geographical_feature_category ,Estuary ,Environmental Exposure ,General Medicine ,Oceanography ,chemistry ,Spain ,Environmental science ,Environmental Pollutants ,Environmental Monitoring - Published
- 2002
44. [Untitled]
- Author
-
Abelardo Gómez-Parra, Tomás Ángel DelValls, Jesús M. Forja, and Inmaculada Riba
- Subjects
Pollution ,Hydrology ,geography ,geography.geographical_feature_category ,National park ,media_common.quotation_subject ,Environmental engineering ,Sediment ,Estuary ,General Medicine ,Management, Monitoring, Policy and Law ,engineering.material ,Acid mine drainage ,engineering ,Environmental science ,Ecotoxicology ,Ecosystem ,Pyrite ,General Environmental Science ,media_common - Abstract
The Donana National Park is one of the most important Natural Reserves in west Europe. The park and the Guadalquivir estuary were impacted by the release of 5 million cubic meters of acid waste from the processing of pyrite ore. Here are presented a multivariate analysis approach to evaluate the extension and the magnitude of the accidental spill on the estuarine sediments. Sediments were used to analyze 6 different metals (Fe, Mn, Zn, Cd, Pb, Cu). Also the concentration of the metals in the different geochemical sediment fractions were determined as a means of assessing the potential impact of the acid water from the mine. The analysis of the results obtained permits to establish that the impact of the accident was acute on the ecosystem due to the set up of a treatment plan in June 1998 and to the high hydrodynamic of the estuary that absorb the early impact. Chronic contamination by Cu was also detected during the monitoring of the impact of the accident in the estuary but this did not appear to be related to the accidental spill. Because the likelihood of long term adverse effects from the heavy metal contamination of estuarine sediments, further ecotoxicological evaluations should be carried out for the estuary and the park using an integrated approach.
- Published
- 2002
45. Comparative analysis of two weight-of-evidence methodologies for integrated sediment quality assessment
- Author
-
Inmaculada Riba, M. Antequera Ramos, Alla Khosrovyan, Araceli Rodríguez-Romero, Tomás Ángel DelValls, Ministerio de Ciencia e Innovación (España), European Commission, Fundación Universidad Empresa de la provincia de Cádiz, United Nations Educational, Scientific and Cultural Organization, and Junta de Andalucía
- Subjects
Geologic Sediments ,Environmental Engineering ,Multivariate analysis ,Chemical Phenomena ,Health, Toxicology and Mutagenesis ,Biological Availability ,Materials management ,Toxicity Tests ,Sediment contamination ,Environmental Chemistry ,Animals ,Integrated assessment ,Amphipoda ,Robustness (economics) ,WOE ,Weight of evidence ,Principal Component Analysis ,Toxicity ,Quality assessment ,business.industry ,Environmental resource management ,Public Health, Environmental and Occupational Health ,Environmental engineering ,Dredged material ,Sediment ,Polychaeta ,General Medicine ,General Chemistry ,Elutriate ,Pollution ,Aliivibrio fischeri ,Spain ,Paracentrotus ,Environmental science ,Environmental Pollutants ,Risk assessment ,business ,Environmental Monitoring - Abstract
The results of sediment quality assessment by two different weight-of-evidence methodologies were compared. Both methodologies used the same dataset but as criteria and procedures were different, the results emphasized different aspects of sediment contamination. One of the methodologies integrated the data by means of a multivariate analysis and suggested bioavailability of contaminants and their spatial distribution. The other methodology, used in the dredged material management framework recently proposed in Spain, evaluated sediment toxicity in general by assigning categories. Despite the differences in the interpretation and presentation of results, the methodologies evaluated sediment risk similarly, taking into account chemical concentrations and toxicological effects. Comparison of the results of different approaches is important to define their limitations and thereby avoid implications of potential environmental impacts from different management options, as in the case of dredged material risk assessment. Consistent results of these two methodologies emphasized validity and robustness of the integrated, weight-of-evidence, approach to sediment quality assessment. Limitations of the methodologies were discussed., This work was supported by the financial help of the Spanish Ministry of Science and Innovation, Projects CTM2011-28437-C02-02/TECNO, CTM2012-36476-C02-01/TECNO. The first author also thanks the Erasmus Mundus Joint Doctorate program, employer Fundación Universidad Empresa de la Provincia de Cádiz and Catedra UNESCO/UNITWIN_WiCop for the doctoral scholarship and administrative support.The second author also thanks Junta de Andalucía (Spanish regional government) for the Grant RNM-3924.
- Published
- 2014
46. Early Contamination by Heavy Metals of the Guadalquivir Estuary After the Aznalcóllar Mining Spill (SW Spain)
- Author
-
I. Sáenz, Jesús M. Forja, Abelardo Gómez-Parra, Inmaculada Riba, and Tomás Ángel DelValls
- Subjects
Pollution ,Cadmium ,geography ,Suspended solids ,geography.geographical_feature_category ,media_common.quotation_subject ,Environmental engineering ,chemistry.chemical_element ,Sediment ,Estuary ,Aquatic Science ,engineering.material ,Contamination ,Oceanography ,chemistry ,Environmental chemistry ,engineering ,Environmental science ,Pyrite ,Water pollution ,media_common - Abstract
The Guadalquivir estuary was impacted by the accidental release of 5 million cubic meters of acid waste from the processing of pyrite ore (25 April 1998). The waste entered ecologically sensitive and protected areas such as the National Park of Donana. Here are presented the results obtained from a time sequence of different sampling sites in the estuary and in the surrounding areas from May to September 1998. Water, suspended solids and sediments were analysed for six different metals (Fe, Mn, Zn, Cd, Pb, Cu). Also, the concentration of the metals in the different geochemical sediment fractions was determined as a means of assessing bioavailability. The results obtained during the first weeks after the incident show high concentrations of Zn. At the end of June a 10 000 m 3 temporary water-treatment plant was constructed on site for depuration of the toxic waters. The results obtained in August and September show a decrease in the metal concentrations measured in the estuary. Only concentrations of Zn were higher than sediment quality values proposed by various authors.
- Published
- 2000
47. Several benthic species can be used interchangeably in integrated sediment quality assessment
- Author
-
Inmaculada Riba, Mercedes Conradi, R. Obispo, Araceli Rodríguez-Romero, F. Serrano, Alla Khosrovyan, and T.A. Del Valls
- Subjects
Aquatic Organisms ,Geologic Sediments ,Health, Toxicology and Mutagenesis ,Weight-of-evidence ,Biology ,Lugworm ,Sensitivity ,Species Specificity ,Animals ,Amphipoda ,Lethality ,Toxicity ,Ecology ,Quality assessment ,Public Health, Environmental and Occupational Health ,Amphipod ,Sediment ,Polychaeta ,General Medicine ,biology.organism_classification ,Pollution ,Spain ,Benthic zone ,Bioaccumulation ,Arenicola ,Corophium volutator ,Bioindicator ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
The selection of the best management option for contaminated sediments requires the biological assessment of sediment quality using bioindicator organisms. There have been comparisons of the performance of different test species when exposed to naturally occurring sediments. However, more research is needed to determine their suitability to be used interchangeably. The sensitivity of two amphipod species (Ampelisca brevicornis and Corophium volutator) to sediments collected from four different commercial ports in Spain was tested. For comparison the lugworm, Arenicola marina, which is typically used for bioaccumulation testing, was also tested. Chemical analyses of the sediments were also conducted. All species responded consistently to the chemical exposure tests, although the amphipods, as expected, were more sensitive than the lugworm. It was found that C. volutator showed higher vulnerability than A.brevicornis. It was concluded that the three species can be used interchangeably in the battery of tests for integrated sediment quality assessment. © 2013 Elsevier Inc., This work was supported by the Spanish Ministry of Science and Innovation through the grants CTM 2011-2843-CO2-02 and CTM2012-36476-C02-01and by the grant RNM-3924 of the Junta de Andalucía.
- Published
- 2013
48. Comparative performances of eggs and embryos of sea urchin (Paracentrotus lividus) in toxicity bioassays used for assessment of marine sediment quality
- Author
-
F. Serrano, Araceli Rodríguez-Romero, M.J. Salamanca, T.A. Del Valls, Alla Khosrovyan, and Inmaculada Riba
- Subjects
Geologic Sediments ,Embryo, Nonmammalian ,Weight-of-evidence ,Aquatic Science ,Biology ,Test subject ,Oceanography ,Paracentrotus lividus ,Sensitivity ,biology.animal ,Toxicity Tests ,Bioassay ,Animals ,Sea urchin embryo ,Sea urchin ,Ovum ,Toxicity ,Ecology ,Sea urchin egg ,Sediment ,Embryo ,biology.organism_classification ,Pollution ,Environmental chemistry ,Sea Urchins ,embryonic structures ,Biological Assay ,Water Pollutants, Chemical ,Potential toxicity ,Environmental Monitoring - Abstract
The potential toxicity of sediments from various ports was assessed by means of two different liquid-phase toxicity bioassays (acute and chronic) with embryos and eggs of sea urchin Paracentrotus lividus. Performances of embryos and eggs of P. lividus in these bioassays were compared for their interchangeable applicability in integrated sediment quality assessment. The obtained endpoints (percentages of normally developed plutei and fertilized eggs) were linked to physical and chemical properties of sediments and demonstrated dependence on sediment contamination. The endpoints in the two bioassays were strongly correlated and generally exhibited similar tendency throughout the samples. Therein, embryos demonstrated higher sensitivity to elutriate exposure, compared to eggs. It was concluded that these tests could be used interchangeably for testing toxicity of marine sediments. Preferential use of any of the bioassays can be determined by the discriminatory capacity of the test or vulnerability consideration of the test subject to the surrounding conditions. © 2013 Elsevier Ltd.
- Published
- 2013
49. Biodynamic modelling of the accumulation of Ag, Cd and Zn by the deposit-feeding polychaete Nereis diversicolor: Inter-population variability and a generalised predictive model
- Author
-
Julián Blasco, Judit Kalman, Inmaculada Riba, Philip S. Rainbow, and Brian D. Smith
- Subjects
0106 biological sciences ,Geologic Sediments ,Silver ,chemistry.chemical_element ,Zinc ,010501 environmental sciences ,Aquatic Science ,Oceanography ,01 natural sciences ,Models, Biological ,Nereis diversicolor ,Biodynamic modelling ,Predictive Value of Tests ,Animals ,Water pollution ,0105 earth and related environmental sciences ,Polychaete ,Cadmium ,biology ,Ecology ,010604 marine biology & hydrobiology ,Sediment ,Polychaeta ,General Medicine ,Feeding Behavior ,biology.organism_classification ,Pollution ,chemistry ,Bioaccumulation ,Environmental chemistry ,Steady state (chemistry) ,Water quality ,Water Pollutants, Chemical - Abstract
11 páginas, 3 figuras, 8 tablas., Biodynamic parameters of the ragworm Nereis diversicolor from southern Spain and south England were experimentally derived to assess the inter-population variability of physiological parameters of the bioaccumulation of Ag, Cd and Zn from water and sediment. Although there were some limited variations, these were not consistent with the local metal bioavailability nor with temperature changes. Incorporating the biodynamic parameters into a defined biodynamic model, confirmed that sediment is the predominant source of Cd and Zn accumulated by the worms, accounting in each case for 99% of the overall accumulated metals, whereas the contribution of dissolved Ag to the total accumulated by the worm increased from about 27 to about 53% with increasing dissolved Ag concentration. Standardised values of metal-specific parameters were chosen to generate a generalised model to be extended to N. diversicolor populations across a wide geographical range from western Europe to North Africa. According to the assumptions of this model, predicted steady state concentrations of Cd and Zn in N. diversicolor were overestimated, those of Ag underestimated, but still comparable to independent field measurements. We conclude that species-specific physiological metal bioaccumulation parameters are relatively constant over large geographical distances, and a single generalised biodynamic model does have potential to predict accumulated Ag, Cd and Zn concentrations in this polychaete from a single sediment metal concentration., This work was also supported by the Project “Interreg IIIA Cooperación Transfronteriza España-Portugal, FEDER-EU” (SP3.E101/03).
- Published
- 2010
50. Comparative toxicity of cadmium in the commercial fish species Sparus aurata and Solea senegalensis
- Author
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T. Ángel DelValls, Inmaculada Riba, Judit Kalman, and Julián Blasco
- Subjects
Gill ,Gills ,Health, Toxicology and Mutagenesis ,Solea senegalensis ,Longevity ,Fish species ,chemistry.chemical_element ,Food Contamination ,Andrology ,chemistry.chemical_compound ,Flatfish ,Cadmium Chloride ,Sparus aurata ,Toxicity Tests, Acute ,Animals ,Intestinal Mucosa ,Cadmium ,biology ,Ecology ,Public Health, Environmental and Occupational Health ,Aquatic animal ,General Medicine ,biology.organism_classification ,Pollution ,Sea Bream ,Metallothionein-like proteins ,Intestines ,chemistry ,Liver ,Toxicity ,Administration ,Inactivation, Metabolic ,Flatfishes ,Metallothionein ,Biomarkers ,Injections, Intraperitoneal ,Water Pollutants, Chemical ,Toxicant - Abstract
6 páginas, 2 figuras, 1 tabla., The induction of metallothionein-like proteins (MTLPs) as well as cadmium levels (Cd) was studied in tissues of gilthead sea bream Sparus aurata and flatfish Solea senegalensis after an intraperitoneal (i.p.) injection of cadmium (2.5 mg kg−1 body weight). The liver, gills, intestine and blood of S. aurata as well as the liver and intestine of S. senegalensis were collected for analysis at 0, 3 and 6 days after the injection. Cd levels significantly increased in all tissues of the treated animals, and the highest accumulation was found in the liver in both species (p, This work was partially supported by research proposals funded by the Research National Plan (CTM2005-07282-C03-02, CTM2008-06344-C03-03 and CTM2008-06344-C03-02) and by UNESCO UNITWIN_Wicop at the Faculty of Marine and Environmental Sciences (University of Cádiz). J. Kalman thanks the i3p program for funding her research at ICMAN (CSIC). Dr. I. Riba thanks the ‘Ramon y Cajal’ program for funding her research at ICMAN/CSIC.
- Published
- 2009
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