Search

Your search keyword '"dNab2"' showing total 3 results
Start Over Descriptor "dNab2" Topic poly(a) tail
3 results on '"dNab2"'

1. The Conserved, Disease-Associated RNA Binding Protein dNab2 Interacts with the Fragile X Protein Ortholog in Drosophila Neurons

Author

Rick S. Bienkowski, Ayan Banerjee, J. Christopher Rounds, Jennifer Rha, Omotola F. Omotade, Christina Gross, Kevin J. Morris, Sara W. Leung, ChangHui Pak, Stephanie K. Jones, Michael R. Santoro, Stephen T. Warren, James Q. Zheng, Gary J. Bassell, Anita H. Corbett, and Kenneth H. Moberg

Subjects
dNab2, fragile X mental retardation protein, RNA binding protein, poly(A) tail, CamKII, futsch, Drosophila, neurodevelopment, memory, ZC3H14, Biology (General), QH301-705.5
Abstract

The Drosophila dNab2 protein is an ortholog of human ZC3H14, a poly(A) RNA binding protein required for intellectual function. dNab2 supports memory and axon projection, but its molecular role in neurons is undefined. Here, we present a network of interactions that links dNab2 to cytoplasmic control of neuronal mRNAs in conjunction with the fragile X protein ortholog dFMRP. dNab2 and dfmr1 interact genetically in control of neurodevelopment and olfactory memory, and their encoded proteins co-localize in puncta within neuronal processes. dNab2 regulates CaMKII, but not futsch, implying a selective role in control of dFMRP-bound transcripts. Reciprocally, dFMRP and vertebrate FMRP restrict mRNA poly(A) tail length, similar to dNab2/ZC3H14. Parallel studies of murine hippocampal neurons indicate that ZC3H14 is also a cytoplasmic regulator of neuronal mRNAs. Altogether, these findings suggest that dNab2 represses expression of a subset of dFMRP-target mRNAs, which could underlie brain-specific defects in patients lacking ZC3H14.

Published
2017
Full Text
View/download PDF

2. The Conserved, Disease-Associated RNA Binding Protein dNab2 Interacts with the Fragile X Protein Ortholog in Drosophila Neurons.

Author

Bienkowski, Rick S., Banerjee, Ayan, Rounds, J. Christopher, Rha, Jennifer, Omotade, Omotola F., Gross, Christina, Morris, Kevin J., Leung, Sara W., Pak, ChangHui, Jones, Stephanie K., Santoro, Michael R., Warren, Stephen T., Zheng, James Q., Bassell, Gary J., Corbett, Anita H., and Moberg, Kenneth H.

Abstract

Summary The Drosophila dNab2 protein is an ortholog of human ZC3H14, a poly(A) RNA binding protein required for intellectual function. dNab2 supports memory and axon projection, but its molecular role in neurons is undefined. Here, we present a network of interactions that links dNab2 to cytoplasmic control of neuronal mRNAs in conjunction with the fragile X protein ortholog dFMRP. dNab2 and dfmr1 interact genetically in control of neurodevelopment and olfactory memory, and their encoded proteins co-localize in puncta within neuronal processes. dNab2 regulates CaMKII , but not futsch , implying a selective role in control of dFMRP-bound transcripts. Reciprocally, dFMRP and vertebrate FMRP restrict mRNA poly(A) tail length, similar to dNab2/ZC3H14. Parallel studies of murine hippocampal neurons indicate that ZC3H14 is also a cytoplasmic regulator of neuronal mRNAs. Altogether, these findings suggest that dNab2 represses expression of a subset of dFMRP-target mRNAs, which could underlie brain-specific defects in patients lacking ZC3H14. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

3. The Conserved, Disease-Associated RNA Binding Protein dNab2 Interacts with the Fragile X Protein Ortholog in Drosophila Neurons

Author

Gary J. Bassell, Rick Bienkowski, Ayan Banerjee, Stephen T. Warren, Kenneth H. Moberg, Michael R. Santoro, James Q. Zheng, Omotola F. Omotade, Stephanie K. Jones, Anita H. Corbett, Christina Gross, Kevin J. Morris, ChangHui Pak, Jennifer Rha, Sara W. Leung, and J. Christopher Rounds

Subjects
0301 basic medicine, Male, Polyadenylation, RNA-binding protein, poly(A) tail, dNab2, Biology, General Biochemistry, Genetics and Molecular Biology, Article, CamKII, 03 medical and health sciences, Fragile X Mental Retardation Protein, Mice, 0302 clinical medicine, Memory, Ca2+/calmodulin-dependent protein kinase, Cell Line, Tumor, medicine, Animals, Drosophila Proteins, Gene Regulatory Networks, RNA, Messenger, Axon, lcsh:QH301-705.5, Cells, Cultured, Genetics, Regulation of gene expression, Neurons, Messenger RNA, neurodevelopment, RNA, Gene Expression Regulation, Developmental, RNA-Binding Proteins, RNA binding protein, futsch, Smell, 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), nervous system, ZC3H14, Drosophila, Female, Calcium-Calmodulin-Dependent Protein Kinase Type 2, 030217 neurology & neurosurgery, Drosophila Protein
Abstract

The Drosophila dNab2 protein is an ortholog of human ZC3H14, a poly(A) RNA binding protein required for intellectual function. dNab2 supports memory and axon projection, but its molecular role in neurons is undefined. Here, we present a network of interactions that links dNab2 to cytoplasmic control of neuronal mRNAs in conjunction with the fragile X protein ortholog dFMRP. dNab2 and dfmr1 interact genetically in control of neurodevelopment and olfactory memory, and their encoded proteins co-localize in puncta within neuronal processes. dNab2 regulates CaMKII, but not futsch, implying a selective role in control of dFMRP-bound transcripts. Reciprocally, dFMRP and vertebrate FMRP restrict mRNA poly(A) tail length, similar to dNab2/ZC3H14. Parallel studies of murine hippocampal neurons indicate that ZC3H14 is also a cytoplasmic regulator of neuronal mRNAs. Altogether, these findings suggest that dNab2 represses expression of a subset of dFMRP-target mRNAs, which could underlie brain-specific defects in patients lacking ZC3H14.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results