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Your search keyword '"Denisova LIa"' showing total 2 results
Start Over Author "Denisova LIa" Topic poly da-dt
2 results on '"Denisova LIa"'

1. [Termination of DNA transcription by a poly(dA).poly(dT) fragment inserted into pBR325 plasmid].

Author

Denisova LIa, Zagrebel'nyĭ SN, Pustoshilova NM, Putintseva NI, and Puchkova LI

Subjects
Base Composition, Chromosome Mapping, DNA Restriction Enzymes, DNA, Bacterial genetics, Electrophoresis, Agar Gel, Escherichia coli genetics, Nucleic Acid Hybridization, DNA Transposable Elements, DNA, Recombinant, Plasmids, Poly dA-dT genetics, Polydeoxyribonucleotides genetics, Transcription, Genetic
Abstract

A recombinant DNA was constructed by inserting polynucleotide (dA).(dT) of 80-100 base pairs long into EcoRI site of the pBR325 plasmid DNA. Transcription of this DNA was studied in E. coli RNA polymerase system in vitro. Some transcripts obtained with the recombinant plasmid were shown to have poly(U) clusters at the 3'-ends. Obtained data indicate that poly(dA).poly(dT) sequence acts as a terminator of RNA synthesis. Orientation of this sequence in recombinant DNA was also established.

Published
1984

2. [Effect of poly(dA).poly(dT) sequence inserted into EcoR1 site of pBr322 plasmid on the expression of tet and bla genes].

Author

Andreeva IS, Denisova LIa, Zagrebel'nyĭ SN, Kileva EV, and Pustoshilova NM

Subjects
Ampicillin Resistance genetics, Base Sequence, DNA genetics, Electrophoresis, Agar Gel, Plasmids, Promoter Regions, Genetic, Tetracycline Resistance genetics, Transformation, Genetic, Deoxyribonuclease EcoRI, Gene Expression Regulation, Poly dA-dT, Polydeoxyribonucleotides
Abstract

The 99 base pair poly(dA).poly(dT) stretch was inserted into EcoRI site of pBR plasmid. The effect of this insertion on tet-gene expression from P2 promoter, and bla-gene expression from P1 and P3 promoters was studied. The insertion results in a slight enhancement of the resistance of the cells to tetracycline and a decreased sensitivity to ampicillin. It is suggested that these findings reflect the effect of insertion on the transcription of the corresponding genes.

Published
1989

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