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Polycyclic aromatic hydrocarbons (PAHs) and dioxins are potential causes of multiple diseases by activating the aryl hydrocarbon receptor (AhR) pathway. Health risk assessment of chemicals primarily relies on the relative potency factor (RPF), although its accuracy may be limited when solely using EC50 values. The induction of cytochrome P4501A1 (CYP1A1) serves as a biomarker for AhR activation and is an integrator of dioxin-like toxicity. Here, we present a method for evaluating the risks associated with AhR activation using mathematical models of dose-CYP1A1 induction. The dose-effect curves for certain PAHs and dioxins, including Ant, BghiP, 1,2,3,4,7,8-HxCDD, and others, exhibited a non-classical S-shaped form. The toxic equivalent factor (TEF) profiles revealed a broad range of toxic equivalent factor values. The TEFs for PAHs ranged from approximately 0.01 to 6, with higher values being observed when the concentration was less than 10 -10 M, with the exceptions of Ace, Phe, and BghiP. Most congeners of dioxins got the lowest TEF value at around 10 -10 M, ranging from 0.04 to 1.00. The binding affinity of AhR to ligands did not display a strong correlation with the EC50 of CYP1A1 expression, suggesting that the AhR-mediated effects of PAHs and dioxins are not fixed but instead fluctuate with the dose. Air samples acquired from a parking area were used to compare the proficiency of RPF and our current approach. In the current method, naphthalene and chrysene were the primary contributors of PAHs to AhR-mediated risks in parking lots air samples, respectively. However, the contributions of naphthalene and chrysene could be disregarded in the RPF approach., Competing Interests: Declaration of Competing Interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled “A novel approach to assess the health risk of aryl hydrocarbon receptor-bound contaminants via inhalation exposure usingCYP1A1expression as a biomarker”., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

During the process of industrial heating, a large amount of polycyclic aromatic hydrocarbons (PAHs) and their halogenated compounds (Cl/Br-PAHs) can be formed. However, there is still limited understanding of the chemicals from different metal smelting industrial parks. This study evaluated the seasonal variations, composition profiles, and source allocations of the atmospheric particulate-bound PAHs and Cl/Br-PAHs in different metal industrial parks in a typical industrial city in northwest China. The results showed that the main PAHs produced by metal smelting were low molecular weight isomers, and the concentrations of Cl-PAHs were lower compared to Br-PAHs. The main Br-PAHs were 1-Br-Pyr and 4-Br-Pyr, while 9-Cl-Fle, 1-Cl-Pyr, and 6-Cl-BaP were the dominated Cl-PAH isomers. No significant difference was found in the concentrations among the sites, whereas the levels of the target chemicals were higher during cold months compared to warm months. The main source of PAHs was coal combustion and gasoline vehicle emission during metal smelting, and that of Cl/Br-PAHs was also industrial coal burning. In addition to the primary source, the secondary chlorination of parent PAHs was also a significant source of Cl-PAHs in the production of high purity aluminum. This study suggests that Cl-PAHs and Br-PAHs may behave differently in the atmosphere., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The pollution of polycyclic aromatic hydrocarbons (PAHs) and halogenated PAHs (Cl/Br-PAHs) caused by electronic waste dismantling activities have attracted considerable attention. The present study investigated the emissions and formation of PAHs and Cl/Br-PAHs based on the combustion of printed circuit boards simulating electronic waste dismantling process. The emission factor of ΣPAHs was 648 ± 56 ng/g, which was much lower than that of ΣCl/Br-PAHs (8.80 × 10 4  ± 9.14 × 10 3  ng/g). From 25 to 600 °C, the emission rate of ΣPAHs reached a sub-peak of 7.39 ± 1.85 ng/(g•min) at 350 °C, then increased gradually with the fastest rate of 19.9 ± 21.8 ng/(g•min) at 600 °C, whereas that of ΣCl/Br-PAHs was the fastest at 350 °C with a rate of 597 ± 106 ng/(g•min), then decreased gradually. The present study suggested that the formation pathways of PAHs and Cl/Br-PAHs are by de novo synthesis. Low molecular weight PAHs were readily partitioned into gas and particle phases, whereas high molecular weight fused PAHs were only detected in oil phase. However, the proportion of Cl/Br-PAHs in particle and oil phases were different from that of gas phase, whereas similar to that of the total emission. In addition, PAH and Cl/Br-PAH emission factors were used to estimate the emission intensity of pyrometallurgy project in Guiyu Circular Economy Industrial Park, and it was shown that approximately 1.30 kg PAHs and 176 kg Cl/Br-PAHs would be emitted annually. This study revealed that Cl/Br-PAHs would be formed by de novo synthesis, and for the first time provided the emission factors of Cl/Br-PAHs during the heat treatment process of printed circuit board, as well as estimated the contribution of pyrometallurgy, a new electronic waste recovery technology, to environmental Cl/Br-PAH pollution, which provides potential scientific information for governmental decision-making on the control of Cl/Br-PAHs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Occupational workers and residents near petrochemical industry facilities are exposed to multiple contaminants on a daily basis. However, little is known about the co-exposure effects of different pollutants based on biotransformation. The study examined benzo[a]pyrene (BaP), a representative polycyclic aromatic hydrocarbon related to the petrochemical industry, to investigate changes in toxicity and co-exposure mechanism associated with different monoaromatic hydrocarbons (MAHs). A central composite design method was used to simulate site co-exposure scenarios to reveal biotransformation of BaP when co-exposed with benzene, toluene, chlorobenzene, or nitrobenzene in microsome systems. BaP metabolism depended on MAH concentration, and association of MAH with microsome concentration/incubation time. Particularly, MAH co-exposure negatively affected BaP glucuronidation, an important phase Ⅱ detoxification process. BaP metabolite intensities decreased to 43%-80% for OH-BaP-G, and 32%-71% for diOH-BaP-G in co-exposure system with MAHs, compared with control group. Furthermore, glucuronidation was affected by competitive and time-dependent inhibition. Co-exposure significantly decreased gene expression of UGT 1A10 and BCRP/ABCG2 in HepG2 cells, which are involved in BaP detoxification through metabolism and transmembrane transportation. Therefore, human co-exposure to multiple contaminants may deteriorate toxic effects of these chemicals by disturbing metabolic pathways. This study provides a reference for assessing toxic effects and co-exposure risks of pollutants., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Polycyclic aromatic hydrocarbons (PAHs) are a group of environmental endocrine disruptors with known carcinogenic, reproductive, and developmental toxicity. Important knowledge gaps remain regarding the relationship between PAH exposure and unexplained recurrent spontaneous abortion (URSA). In the present study, twelve monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) were measured in the urine of 413 URSA cases and 434 controls. The main OH-PAHs measured in this study were monohydroxy metabolites of naphthalene, followed by fluorene and phenanthrene. After the creatinine correction, the median concentration of urinary OH-PAHs in the control group (17.4 μg/g Creatinine) was higher than that in the case group (14.2 μg/g Creatinine). There was no positive relationship between PAH exposure and URSA using binary logistic regression analysis. Among 847 Chinese women of childbearing age, residential environment, type of drinking water, and education level were the influencing factors of PAH exposure. The health risk assessment showed that over 98% of women had a carcinogenic risk with carcinogenic risk values above the acceptable level (10 -6 ). Although this large-scale case-control study did not observe an association between PAH exposure and URSA, more attention should be paid to the high carcinogenic risk due to PAH exposure in women of reproductive age., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Soil polycyclic aromatic hydrocarbons (PAHs) generated from industrial processes are highly spatially heterologous, with limited quantitative studies on their main influencing factors. The present study evaluated the soil PAHs in three types of industrial parks (a petrochemical industrial park, a brominated flame retardant manufacturing park, and an e-waste dismantling park) and their surroundings. The total concentrations of 16 PAHs in the parks were 340-2.43 × 10 3 , 26.2-2.63 × 10 3 , and 394-2.01 × 10 4  ng/g, which were significantly higher than those in the surrounding areas by 1-2 orders of magnitude, respectively. The highest soil PAH contamination was observed in the e-waste dismantling park. Nap can be considered as characteristic pollutant in the petrochemical industrial park, while Phe in the flame retardant manufacturing park and e-waste dismantling park. Low molecular weight PAHs (2-3 rings) predominated in the petrochemical industrial park (73.0%) and the surrounding area of brominated flame retardant manufacturing park (80.3%). However, high molecular weight PAHs (4-6 rings) were enriched in the other sampling sites, indicating distinct sources and determinants of soil PAHs. Source apportionment results suggested that PAHs in the parks were mainly derived from the leakage of petroleum products in the petroleum manufacturing process and pyrolysis or combustion of fossil fuels. Contrarily, the PAHs in the surrounding areas could have been derived from the historical coal combustion and traffic emissions. Source emissions, wind direction, and local topography influenced the PAH spatial distributions., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Primitive electronic waste (e-waste) dismantling activities have been shown to be an important emissions source for a variety of toxic organic compounds, including carcinogenic polycyclic aromatic hydrocarbons (PAHs). Previous studies have found that some nitrated PAHs (NPAHs) are more toxic than their parent PAHs, however, little attention has been paid to the formation of PAH derivatives during e-waste processing and there is a lack of comprehensive data from field observations. In this study, the spatial distribution, temporal trends and atmospheric fate of NPAHs and hydroxylated PAHs (OH-PAHs) were investigated at typical e-waste dismantling sites, with monitoring data collected over three consecutive years. Compared to background levels, higher levels of NPAHs and OH-PAHs were found in air samples from an e-waste dismantling industrial park, with their seasonal and annual changes shown to be affected by e-waste dismantling activities. Atmospheric PM 2.5 particles were found to have high relative abundances of NPAHs (76.9%-95.1%) and OH-PAHs (73.3%-91.6%), with particle-bound concentrations ranging from 20.1 to 88.8 and 37.1 to 107 pg m -3 , respectively. The most abundant NPAH isomers were found to be 9-Nitroanthracene and 2-Nitrofluoranthene, while OH-PAH isomers containing 2-4 rings were predominant. Source identification was performed based on the specific diagnostic ratios of NPAH isomers, confirming that NPAH and OH-PAH emissions have multiple sources, including emissions related to the e-waste dismantling process, atmospheric photochemical reactions and traffic emissions. Further research on the fate of such derivatives and their potential use as markers for source identification, is urgently required., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Halogenated polycyclic aromatic hydrocarbons (Cl/Br-PAHs) have received tremendous attention due to their high toxicity. To identify the emission pattern of Cl/Br-PAHs from various industrial productions, understand the formation mechanisms and the influence on the surroundings, this study investigated the surface soils of three typical industrial parks. Generally, traces of Cl-PAHs were much lower than Br-PAHs. The mean Cl-PAH concentrations followed the trend of petrochemical industrial park (3.12 ng/g), brominated flame retardant (BFR) manufacturing park (1.48 ng/g), and electronic waste dismantling park (0.26 ng/g). However, the BFR manufacturing park had the highest mean Br-PAH concentration (21.6 ng/g), significantly higher than the other two parks. Generally, higher levels of the chemicals were found in the parks than in their surroundings, except for the electronic waste dismantling park. The massive addition of chlorine additives in crude oil and its by-products, plus the enormous quantity of brominated brines used in BFR productions, favor Cl/Br-PAH formation. Analyzing the homolog compositions of Cl/Br-PAHs suggested that 3- or 4-ring Cl/Br-PAHs were typically come from the petrochemical industrial park and electronic waste dismantling park. Contrarily, 4- or 5-ring Cl/Br-PAHs were predominantly come from the BFR manufacturing activity. This study provides fingerprints to trace the Cl/Br-PAH emissions during industrial production and analyzes the formation mechanism., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Assessing the adverse health risks at molecular endpoints to various aquatic organisms could be an urgent issue. In this manuscript, the ecological and AhR-mediated risk of sixteen polycyclic aromatic hydrocarbons (PAHs) and six polybrominated diphenyl ethers (PBDEs) in surface water of Dongjiang River, Southern China was evaluated using chemical analysis and in silico approaches. Average concentrations of ∑ 16 PAHs and ∑ 6 PBDEs were 586.3 ng/L and 2.672 ng/L in the dry season (DS), and 366.8 ng/L and 2.554 ng/L in the wet season (WS). Concentrations of PAHs during the DS were significantly higher than that in the WS, while no obvious seasonal distribution was observed for PBDEs. Only Ant and BaP in all congers of PAHs posed low to medium ecological risks, and PBDEs posed a low ecological risk. Moreover, AhR-mediated risk from PAHs was two orders of magnitude higher that from PBDEs, and the AhR-mediated toxicity on frog and eel were higher than those on other aquatic organisms in Dongjiang River. Phe and BDE209 were the significant contributor to the AhR-mediated risk induced by PAHs and PBDEs, respectively. This study is the first attempt to assess AhR-mediated risk of river water in multiple aquatic organisms., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Electronic waste (e-waste) is one of the fastest-growing solid wastes and has become an urgent issue due to the potential adverse consequences of exposure to emitted toxic pollutants, especially for these occupational exposed workers and local residents. In this review, the environmental occurrences, emission characteristics, sources, and possible adverse effects of polycyclic aromatic hydrocarbons (PAHs) emitted from primitive e-waste dismantling activities are summarized. In general, the atmospheric levels of PAHs at typical e-waste sites, e.g., in Guiyu, China, have substantially decreased by more than an order of magnitude compared with levels a decade ago. The PAH concentrations in soil from old e-waste sites in China are also generally lower than those at newly emerged e-waste sites in India, Pakistan and Ghana. However, elevated concentrations of PAHs have been reported in human milk, hair and urine from the populations near these e-waste sites. Source apportionment both from bench-scale studies to field observations has demonstrated that the pyrolysis and combustion processing of electronic circuit board are mainly responsible for the emissions of various PAHs. In addition, some specific PAHs and their derivatives, such as triphenylbenzene, halogenated and oxygenated PAHs, have frequently been identified and could be considered as indicators in routine analysis in addition to the 16 U.S. EPA priority PAHs currently used., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Polycyclic aromatic hydrocarbons (PAHs) derived from the incomplete combustion of organic materials are associated with adverse health effects. However, little is known about PAH exposure levels and their influencing factors on a large scale in developing countries. In this study, urinary monohydroxylated metabolites of PAHs (OH-PAHs), including the metabolites of naphthalene, fluorene, phenanthrene, pyrene, chrysene, and benzo[a]pyrene, were measured in 1154 samples in the general population nationwide from 26 provincial capitals in China. Concentrations of OH-PAHs ranged from 1.39 to 228 μg/L. OH-Nap, metabolite of naphthalene, was the predominant compound, accounting for 65.1% of totals. People in eastern, southwest and northeast China, such as Shanghai, Kunming, Nanning, and Changchun, suffered more PAH exposure than other regions which might associate with sampling time, living habits of the subjects, and the imbalance of economic development and energy consumption across regions. Urinary OH-PAH concentrations were associated with body mass index, gender, and age, and smoking was the main correlating factor. Inhalation and diet might be the main exposure route of human exposure to PAHs, especially for smokers by inhalation. Hazard indices showed that no subject was exposed to PAHs with potential non-carcinogenic risk. Furthermore, the carcinogenic risk was the most significant health effects, with almost all subjects having carcinogenic risk values higher than the acceptable level of 10 -6 . Naphthalene and phenanthrene were the main contributors. The results also suggested a possible relationship between PAH exposure and lung cancer in the Chinese population. This first nationwide study on human internal exposure to PAHs provides a large body of scientific information for governmental decision-making about associated human health and the prevention of human exposure to PAHs., (Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

A method was developed for the simultaneous determination of polycyclic aromatic hydrocarbons (PAHs) and their hydroxylated metabolites (OH-PAHs) in human fingernails using liquid-liquid extraction and online purification. After surface decontamination by rinsing with acetone, the fingernails were digested with sodium hydroxide and subjected to liquid-liquid extraction with a mixture of n-hexane and methyl tertbutyl ether. The organic extract was then fractionated using a silica-based solid-phase extraction (SPE) cartridge to obtain a PAH fraction eluted with n-hexane/dichloromethane (v/v, 95:5) and an OH-PAH fraction eluted with dichloromethane/ethyl acetate (v/v, 50:50). The PAH fraction was directly injected into an online gel permeation chromatography-gas chromatography-triple quadrupole tandem mass spectrometry (GPC-GC-MS/MS) system, enabling rapid determination of 16 PAHs. A parallel online SPE liquid chromatography tandem mass spectrometry (LC-MS/MS) method was used to determine 12 OH-PAHs. Validation experiments showed that the recovery of PAH and OH-PAH were within range of 67.4%-105.1% (RSD ≤ 10.1%) and 72.8%-102.3% (RSD ≤ 10.9%), respectively, with limits of quantitation (LOQ) of 0.06-0.8 ng/g and 0.15-3.1 ng/g, respectively. Forty-two human fingernail samples from residents of Southern China were analyzed to establish background PAH and OH-PAH levels in this region. Several PAHs and OH-PAHs were detected, at concentrations of 97.5 to 3,687 ng/g for PAHs and 24.2 to 767 ng/g for OH-PAHs. The dominant homologues were two- and three-ring PAH isomers, notably naphthalene (Nap), fluorene (Flu), and phenanthrene (Phe), as well as the corresponding hydroxylated metabolites 2-OH-Nap, OH-Flu, and OH-Phe. Smoking, consuming barbecued food, and age had no significant effects on PAH exposure, but a larger sample would be required to confirm this finding. The online purification strategy presented here expedites cleanup and purification during analysis of human fingernails and should facilitate non-invasive biomonitoring of PAHs in humans, particularly when analyzing large numbers of samples., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Polycyclic aromatic hydrocarbons (PAHs) are compounds with two or more benzene rings whose hydroxylated metabolites (OH-PAHs) are excreted in urine. Human PAH exposure is therefore commonly estimated based on urinary OH-PAH concentrations. However, no study has compared PAH exposure estimates based on urinary OH-PAHs to measurements of PAH levels in blood samples. Estimates of PAH exposure based solely on urinary OH-PAHs may thus be subject to substantial error. To test this hypothesis, paired measurements of parent PAHs in serum and OH-PAHs in urine samples from 480 participants in Guangzhou, a typical developed city in southern China, were used to investigate differences in the estimates of human PAH exposure obtained by sampling different biological matrices. The median PAH concentration in serum was 4.05 ng mL -1 , which was lower than that of OH-PAHs in urine (8.33 ng mL -1 ). However, serum pyrene levels were significantly higher than urinary levels of its metabolite 1-hydroxypyrene. Concentrations of parent PAHs in serum were not significantly correlated with those of their metabolites in urine with the exception of phenanthrene, which exhibited a significant negative correlation. Over 28% of the participants had carcinogenic risk values above the acceptable cancer risk level of 10 -6 . Overall, estimated human exposure and health risks based on urinary 1-hydroxypyrene levels were only 13.6% of those based on serum pyrene measurements, indicating that estimates based solely on urine sampling may substantially understate health risks due to PAH exposure., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Dermal exposure to semivolatile organic compounds (SVOCs) has recently attracted widespread attention; understanding these exposures is particularly important for people whose skin is frequently exposed to different pollution surfaces. In this study, handwipes were collected from exposed occupational workers and local residents near a typical electronic waste (e-waste) dismantling area; urine samples were also sampled. The wipes were analyzed for three typical SVOCs: polybrominated diphenyl ethers (PBDEs), polycyclic aromatic hydrocarbons (PAHs), and organophosphate flame retardants (OPFRs). The median levels of PAHs, OPFRs, and PBDEs in handwipes from e-waste dismantlers were 96.0, 183, and 238 ng, respectively. The analytes were higher in the handwipes collected from workers than those from residents, indicating that they were subjected to greater dermal exposure during primitive e-waste dismantling activities. Among the three SVOCs, the strongest correlation was found between triphenyl phosphate (TPhP) in handwipes and diphenyl phosphate (DPhP) in paired urine; the next strongest correlations were between PAHs and PBDEs and their corresponding urinary metabolites. The results showed that TPhP contributed the highest exposure to e-waste dismantlers via dermal exposure. Our research highlights the importance of dermal exposure to TPhP, which should be considered in future exposure risk assessments.

Non-invasive human biomonitoring methods using hair and fingernails as matrices are widely used to assess the exposure of organic contaminants. In this work, a total of 72 human fingernails were collected from workers and near-by residents from a typical electronic waste (e-waste) dismantling site, and were analyzed for human exposure to polycyclic aromatic hydrocarbons (PAHs) and their mono-hydroxyl metabolites (OH-PAHs). The concentrations of PAHs and OH-PAHs were obtained as 7.97-551 and 39.5-3280 ng/g for e-waste workers (EW workers), 7.05-431 and 27.3-3320 ng/g for non-EW workers, 7.93-289 and 124-779 ng/g for adult residents, and 8.88-1280 and 181-293 ng/g for child residents, respectively. The composition profiles of PAHs in the human fingernails of the four groups were similar, with isomers of Phe, Pyr and Fluo being the predominated congeners, while 2-OH-Nap accounted for more than 70% of the total OH-PAHs. These contaminants were found most in the fingernails of EW workers, followed by non-EW workers, adult residents, and child residents, indicating e-waste dismantling activities are the major sources of PAH exposure. However, significantly higher levels of PAHs with 4-6 rings were observed only in workers as opposed to the residents, and a significant correlation between 3-OH-Flu (p < 0.05) and 2-OH-Phe (p < 0.01) in the fingernails and urine was observed, but no significant correlation was found between the concentration of OH-PAHs in matched hair and fingernail samples. In addition, the levels of PAHs in fingernails increased with the age of EW workers. This is the first study to explore the accumulation and distribution of PAHs and OH-PAHs in human fingernails, which would provide valuable insight into non-invasive biomonitoring and health risk assessment of PAHs., (Copyright © 2021. Published by Elsevier Ltd.)

Polycyclic aromatic hydrocarbons (PAHs) and certain ingredients in personal care products, such as parabens, bisphenols, triclosan and phthalate metabolites, have become ubiquitous in the world. Concerns of human exposure to these pollutants have increased during recent years because of various adverse health effects of these chemicals. Multiple compounds including parabens, bisphenols, triclosan, phthalate metabolites (mPAEs) and hydroxyl PAHs (OH-PAHs) in urine samples from Guangzhou were determined simultaneously to identify the human exposure pathways without external exposure data combined with data analysis, and the toxicants posed the highest risk to human health were screened in the present study. The detection frequencies for the chemicals exceeded 90%. Among the contaminants, mPAEs showed the highest concentrations, followed by OH-PAHs, with triclosan present at the lowest concentrations. Mono-n-butyl phthalate, methylparaben, bisphenol A, and hydroxynaphthalene represented the most abundant mPAE, parabens, bisphenol, and OH-PAH compounds, respectively. The present PAHs are mainly exposed to human through inhalation, while the chemicals added to personal care products are mainly exposed to human through oral intake and dermal contact. The urine samples from suburban subjects showed significantly higher OH-PAH levels than the urine samples from urban subjects, and females had lower OH-PAH levels than males. Urinary concentrations of the analyzed contaminants were significantly correlated with age, body mass index, residence time, as well as the frequencies of alcohol consumption and swimming. Risk assessments based on Monte Carlo simulation indicated that approximately 30% of the subjects suffered non-carcinogenic risks from mPAEs and OH-PAHs, with mPAEs accounting for 89% of the total risk., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs), phthalate metabolites (mPAEs), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the urine of school children aged 8-11 years from Shenzhen, China were measured in order to investigate oxidative stress damage from co-exposure to PAHs and PAEs. The concentrations of OH-PAHs and mPAEs in urine were 0.36-36.5 (median: 3.86) and 9.48-1609 (median: 240) ng/mL respectively. Gender and age did not influence urinary concentrations of ΣOH-PAHs and ΣmPAEs, but geographical variations (i.e., urban versus suburban) were observed. Levels of 8-OHdG were positively correlated with urinary OH-PAHs and mPAEs, with correlation coefficients (r) varying between 0.160 and 0.365 (p < 0.05). OH-PAHs made a greater contribution to oxidative DNA damage than mPAEs when these two types of pollutants were present at the same concentrations. Human health risks were assessed using the hazard quotient and the hazard index for the cumulative risk of a complex of chemicals. The results demonstrated that risks from PAHs could be neglected, but that 29.5 % of school children may be subject to obvious health risks from PAEs, especially diethylhexyl phthalate., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Extensive electronic waste (e-waste) recycling might be an important emission source of polycyclic aromatic hydrocarbons (PAHs) mixture, which might induce negative effects on the employees. In the present work, atmospheric pollution patterns of PAHs and their derivatives were determined in five different workshops to dismantle waste printed circuit boards (WPCBs) via thermal treatment. The results showed that mass concentrations of PAHs, chlorinated PAHs (ClPAHs), brominated PAHs (BrPAHs), oxy-PAHs (OPAHs) as well as carbazole (CBZ) were ranged from 1.53 × 10 4 -2.02 × 10 5 , 32.3-364, 8.29-1.13 × 10 3 , 923-1.39 × 10 4 and 225-1.95 × 10 3 pg·m -3 , respectively. Electric heating furnaces (EHF) workshops emitted relatively higher contaminants than other disposal sectors. OPAHs was found to be the most predominant derivatives of PAHs with 9,10-anthraquinone (83.0%) has the absolute superior in EHFTV, while benzo(a)anthracene-7,12-dione (>45.0%) was found to be the highest congener in other workshops, respectively. 9,10-Cl 2 Phe exhibited the largest contributions to the ΣClPAHs whereas the composition profiles of BrPAHs varied among five workshops. In addition to direct chlorination of parent PAHs, thermal degradation of halogenated flame retardants incorporated into plastic materials might dominate the generation of Cl/BrPAHs from e-waste dismantling activities. The specific isomeric ratios of BrPAHs (3-BrFlu/1-BrPyr and 1-BrPyr/3-BrFlu) might be used to discriminate other emission sources from pyrolysis of WPCBs. However, their specific application as novel tracers for source identification should be further verified with more studies. The emitted PAHs mixture with their derivatives in all dismantling workshops posed carcinogenic risks to these dismantling workers via inhalation, particularly the workshop using electric heating furnaces to treat router. Nevertheless, new loadings of PAHs derivatives observed from e-waste dismantling activities, as well as their comprehensive health risk assessment provides us with a fresh perspective on the source appointment and potential adverse consequences of PAHs. More attention needs to be paid to the potential carcinogenic risks of exposure to PAHs and their derivatives from e-waste dismantling processes., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

The toxicities of some chlorinated and brominated polycyclic aromatic hydrocarbons (X-PAHs) are higher than their corresponding parent PAHs. However, the identification and quantitation of X-PAHs in environment are still changeable and limitedly reported. To develop a robust method for routine analysis of X-PAHs in environmental samples, the determination of 34 X-PAHs was performed and compared using different instruments, including gas chromatography-mass spectrometry (GC-MS), gas chromatography-tandem mass spectrometry (GC-MS/MS) in both electron ionization (EI) and negative chemical ionization (NCI) modes, and comprehensive two-dimensional gas chromatograph-tandem mass spectrometer (GC × GC-MS/MS). GC-EI-MS/MS possessed the highest sensitivity with method detection limits of 2.00-40.0 and 2.00-20.0 pg/g dry weight (dw) for Cl-PAHs and Br-PAHs, respectively. This validated method was then applied to analyze X-PAHs in indoor dusts from a typical e-waste dismantling workshop, and the concentrations of Σ 18 Br-PAHs (8.80-399 ng/g dw) were higher than Σ 16 Cl-PAHs (7.91-137 ng/g dw). The toxicity equivalency quantities (TEQs) of Cl-PAHs at e-waste dismantling workshop and Br-PAHs at raw materials crushing workshop showed the highest values of 176 and 453 pg·TEQ/g, respectively. Cl-PAHs and Br-PAHs posed a potential health risk to workers through dust ingestion in workshops. Further attention should be payed to the formation mechanism of X-PAHs and the health risk., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The main objectives of the present study were to investigate urinary monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in 77 primiparas who live in Shenzhen, Guangdong Province, China, and their association with 8-hydroxy-2'-deoxyguanosine (8-OHdG) and human health risks. High detection frequencies of OH-PAHs demonstrated the wide occurrence of chemicals in the human exposure to PAHs. The urinary concentrations of Σ 7 OH-PAHs ranged from 1.37 to 45.5 ng/mL, and the median concentrations of 1-hydroxynaphthalene (1-OHN), 2-hydroxynaphthalene (2-OHN), 2-hydoxyfluorene (2-OHF), ΣOHPhe (the sum of 1-, 2+ 3-hydroxyphenanthrene), and 1-hydroxypyrene (1-OHP) were 3.00, 2.58, 0.31, 0.44, and 0.51 ng/mL, respectively. In the sum concentration of seven OH-PAHs, 1-OHN accounted for the largest proportion (43.7% of Σ 7 OH-PAHs), followed by 2-OHN (37.1%), 2-OHF (4.94%), 1-OHP (8.01%), 1-OHPhe (4.79%), and 2+3-OHPhe (1.46%). The present results showed that vehicle exhaust and petrochemical emission are the main sources of PAHs in primiparas in Shenzhen, and inhalation is the most important exposure route. The living conditions have a significant influence on human exposure to PAHs. The concentrations of 8-OHdG were positively correlated with OH-PAH concentrations in urine because evidence suggested that urinary 8-OHdG levels can be considered as a biomarker of oxidative DNA damage. Hazard quotient was used to assess the human health risks from exposure to single compound, and hazard index was used to assess the cumulative risks of the compounds, which demonstrated that the exposure risks from PAHs in primiparas were relatively low., Competing Interests: Declaration of competing interest Compliance with ethical standards. The authors declare no conflict of interest., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

In this study, 96 pairs of hair and urine samples were collected from e-waste (EW) dismantling workers of an industrial park, as well as residents living in surrounding areas. The concentrations of polycyclic aromatic hydrocarbons (PAHs) and hydroxylated PAH metabolites (OH-PAHs) were analyzed . The results show that concentrations of Σ 15 PAHs ranged from 6.24 to 692 ng/g dry weight (dw) and Σ 12 OH-PAHs from undetected to 187 ng/g dw in hair external (hair-Ex), and ranged from 31.7 to 738 ng/g dw and 21.6 to 1887 ng/g dw in hair internal (hair-In). There was no significant difference in exposure levels between EW dismantling workers and residents of the surrounding area. For the parent PAHs, the concentrations of Σ 15 PAHs of hair-In were comparable with those of hair-Ex for all populations except for the child residents. On the contrary, for the OH-PAHs, the concentrations of Σ 12 OH-PAHs of hair-In were 9-37 times higher than those of hair-Ex for populations. Moreover, the congener profiles of OH-PAHs of hair-In were different from those of hair-Ex, but similar to that of urine. Particularly, 3-OH-Bap, which is a carcinogenic metabolite, was only detected in the hair-In. These results indicate that OH-PAHs in hair-In, just like in urine, are mainly derived from endogenous metabolism and could be considered as reliable biomarkers for PAHs exposure. However, there was almost no significant correlations between hair-In and urine for OH-PAHs. This indicates that more attention should be paid to OH-PAHs when conducting PAHs exposure risk assessment using hair, which will help to obtain more reliable and comprehensive information on health risk assessments., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Thermal desorption (TD) is a universal solvent-free pre-concentration technique. It is often used to pre-concentrate semi-volatile and volatile organic compounds in various sample types. Polycyclic aromatic hydrocarbons (PAHs) are widespread contaminants from incomplete combustion of organic matter and fossil fuel, which have carcinogenic effects on human health. Conventional methods for determining PAHs, represented by solvent extraction, are gradually being replaced by solvent-free methods, typically the TD technique, because of TD's many advantages, including time savings and environmentally friendly treatment. This work presents an extensive review of the universal methods used to determine PAHs in the atmosphere based on the TD technique. The methods currently used for collection and detection of both gas- and particle-phase PAHs in the air are critically reviewed. In addition, the operating parameters of the TD unit are summarized and discussed. The design shortcomings of existing studies and the problems that researchers should address are presented, and promising alternatives are suggested. This paper also discusses important parameters, such as reproducibility and limit of detection, that form a crucial part of quality assurance. Finally, the limitations and the future prospects of the TD technique for use in airborne PAH analyses are addressed. This is the first review of the latest developments of the TD technique for analysis of PAHs and their derivatives in the atmosphere., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Due to the development of the economy, electronic waste (e-waste) has become a new global problem and e-waste dismantling processes are an important source of air pollution. Among the pollutants emitted, polycyclic aromatic hydrocarbons (PAHs) are a severe concern because of their carcinogenic and mutagenic properties. However, few studies have investigated the atmospheric PAHs generated by e-waste dismantling in a specific region, especially the PAH levels throughout the year. Thus, we assessed the effects of PAHs on the local air quality by sampling the total suspended particulates (TSP), PM 10 , PM 2.5 , and gaseous phase from an e-waste dismantling area and a control site during four seasons. The TSP, PM 10 , and PM 2.5 concentrations were measured as 84.8-414, 70.7-302, and 57.1-204 μg m -3 , respectively, in this area, and those of three types of particulate bound-PAHs and gaseous phase PAHs were 2.6-16.1, 2.2-15.1, 1.9-14.6, and 20.1-72.8 ng m -3 , respectively. The pollutant levels were higher in the spring and winter than those in the summer and autumn. The PAH sources were identified by diagnostic ratio approaches and principal component analysis. E-waste dismantling was identified as the major source of PAH pollution within this area, where approximately 82.4% of the PAHs was attributed to e-waste dismantling at an industrial park (EP site). Among the sites sampled, the pollutant levels and cancer risk were highest at the EP site, and they could pose a cancer risk for humans, although only the bioaccessible PAHs in human lungs were considered. In particular, infants had a higher health risk than adults, thereby suggesting that air pollution with PAHs is a concern in this area. This study provides clear evidence of the requirement for control measurements of e-waste dismantling processes., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The bioaccessibility of organic pollutants is a key factor in human health risk assessments. We developed a novel in vitro method for determining the mass fraction of bioaccessible atmospheric polycyclic aromatic hydrocarbons (PAHs) using an air-washing device containing simulated human lung fluid. The experimental parameters were optimized based on the deposition fractions (DFs) of PAHs in human lung fluids. The DFs were measured for PAHs based on the mass of compounds in the mainstream and exhaled cigarette smoke. The mass fractions of bioaccessible PAHs were measured by passing the mainstream cigarette smoke through the air-washing device, and they were calculated via a simple mass balance equation based on the PAHs in the fluid and mainstream cigarette smoke. The DFs of individual PAHs ranged from 20.5% to 78.1%, and the bioaccessible mass fractions varied between 45.5% and 99.8%. The octanol-water partition coefficients (K OW ) significantly influenced both the DFs and bioaccessible mass fractions of PAHs, and the optimized in vitro method could be used to estimate the bioavailable atmospheric PAHs. This in vitro method can potentially be used to measure the mass fraction of bioaccessible atmospheric PAHs and to assess the health risk related to human exposure to airborne PAHs., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Prenatal exposure to polycyclic aromatic hydrocarbons (PAHs) is a high-priority public health concern. However, maternal to fetal transplacental transfer of PAHs has not been systematically studied. To investigate the transplacental transfer of PAHs from mother to fetus and determine the influence of lipophilicity (octanol-water partition coefficient, K OW ) on transfer process, in the present study, we measured the concentrations of 15 PAHs in 95 paired maternal and umbilical cord serum, and placenta samples (in total 285 samples) collected in Shanghai, China. The average concentration of total PAHs was the highest in maternal serums (1290 ng g -1 lipid), followed by umbilical cord serums (1150 ng g -1 lipid). The value was the lowest in placenta samples (673 ng g -1 lipid). Low molecular weight PAHs were the predominant compounds in the three matrices. Increases in fish and meat consumption did not lead to increases in maternal PAH levels, and no obvious gender differences in umbilical cord serums were observed. The widespread presence of PAHs in umbilical cord serums indicated the occurrence of transplacental transfer. The ratios of PAH concentrations in umbilical cord serum to those in maternal serum (F/M) and the concentrations in placenta to those in maternal serum (P/M) of paired samples were analyzed to characterize the transfer process of individual PAHs. Most F/M ratios on lipid basis were close to one (range: 0.79 to 1.36), which suggested that passive diffusion may control the transplacental transfer of PAHs from maternal serum to the fetal circulation. The P/M and F/M values calculated on lipid basis showed that PAHs with lower K OW were more likely to transfer from mother to fetus via the placenta., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

A total of 16 priority polycyclic aromatic hydrocarbons (PAHs) in sediment samples from Taihu Lake were analyzed by instruments, and sediment extracts were assayed for aryl hydrocarbon receptor (AhR)-mediated ethoxyresorufin-o-deethylase (EROD) induction using a rat hepatoma cell line (H4IIE). The cause-effect relationship between the observed EROD activity and chemical concentrations of PAHs was examined. Our results showed that sediment extracts could induce significant AhR effects, and the bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents of raw extracts (TEQ(bio)s) ranged from 2.7 to 39.8 pg g(-1) dw. Chemical analysis showed that 16 PAHs were all detected in all samples, and their total concentrations (Σ16PAHs) ranged from 179.8 to 1,669.4 ng g(-1) dw. The abundance of sedimentary PAHs in the three regions (Meiliang Bay, Gonghu Bay, and Xukou Bay) showed a decreasing trend from the inflow region to the outflow region. Chemical analysis-derived TEQs (TEQ(cal)s) contributed by PAHs ranged from 1.6 to 20.7 pg g(-1) dw. The mean contribution rates (CRs) of PAHs to TEQ(bio)s were 48.9%. In Meiliang Bay, EROD effects of 60% samples were caused by PAHs whose CRs were more than 60%, while in most sampling sites of Gonghu Bay and Xukou Bay, the CRs of PAHs to TEQ(bio)s were basically below 40%. In addition, preliminary ecological risk assessment found that PAHs in sediments have very low ecological impact based on the chemical data of PAHs, while the sediments might pose an unacceptable risk to aquatic organisms and their predators based on the data of TEQ(bio). These findings showed that EROD effects of sediment extracts from Taihu Lake were also caused by other compounds, such as dioxins, polychlorinated biphenyls, etc., together.

A new method for the simultaneous detection of 20 polybrominated diphenyl ethers (PBDEs), 16 polycyclic aromatic hydrocarbons (PAHs), 4 hydroxyl PBDEs (OH-PBDEs) and 10 hydroxyl PAHs (OH-PAHs) in human hair has been developed for the first time. External target analytes from hair (hair-Ex) were ultrasonically extracted with acetone, while the internal target analytes (hair-In) were obtained with further digestion and liquid–liquid extraction of washed hair. Alkaline digestion with liquid–liquid extraction under alkaline and re-acidification combination conditions was the key procedure to successfully extract both parent and metabolic compounds from hair. Both external and internal extracts were purified with gel permeation chromatography, and the parent compounds were subsequently separated from their hydroxylated metabolites with a silica solid phase extraction column prior to instrumental analysis. GC-MS-MS, GC-MS and HPLC-MS-MS were used to analyze PAHs, PBDEs and their hydroxylated metabolites, respectively. The method showed satisfactory accuracy as well as precision, and the recoveries of PBDEs, PAHs, OH-PBDEs and OH-PAHs ranged from 62%–145%, 48%–135%, 60%–146% and 60%–88%, respectively. The developed method was validated in a pilot biomonitoring campaign. All parent analytes were approximately 100% detected in both hair-In and hair-Ex, while no OH-PBDEs were detected in hair-In and hair-Ex. All OH-PAHs were approximately 100% detected in hair-In with a mean Σ10OH-PAHs concentration of 174.7 ng per g dry weight (dw), and the concentration in hair-Ex was 18 times lower than that in hair-In with a relatively lower detection frequency. Both partial least squares discriminant analysis (PLS-DA) and Spearman correlation analysis with the concentration of analytes confirmed that the developed method performed well to distinguish the internal from external exposure to target analytes in hair. [ABSTRACT FROM AUTHOR]

Highlights: [•] PBDE bioaccessibility in size-specific dust from air conditioner filter were measured. [•] The bioaccessibility of PBDE congeners decreased with increasing of dust size. [•] The way of being of PBDEs in dust significantly influenced the bioaccessibility. [•] Pore volume and OM were the most important factors influencing bioaccessibility. [ABSTRACT FROM AUTHOR]