5 results on '"Miragliotta, G."'
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2. Detection of Bartonella henselae and Afipia felis DNA by polymerase chain reaction in specimens from patients with cat scratch disease.
- Author
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Del Prete R, Fumarola D, Fumarola L, and Miragliotta G
- Subjects
- Adolescent, Adult, Afipia genetics, Bartonella henselae genetics, Cat-Scratch Disease microbiology, Child, Child, Preschool, DNA, Bacterial analysis, Female, Humans, Lymph Nodes microbiology, Male, Nucleic Acid Hybridization methods, Afipia isolation & purification, Bartonella henselae isolation & purification, Cat-Scratch Disease diagnosis, DNA, Bacterial blood, Polymerase Chain Reaction methods
- Abstract
Polymerase chain reaction (PCR) amplification and colorimetric identification of amplicons were performed to detect Bartonella henselae and Afipia felis DNA in specimens from patients who were clinically and histologically suspected of having cat scratch disease. PCR products were revealed using 2% ethidium bromide agarose-gel electrophoresis and identified with specific probes in a commercial colorimetric hybridization assay (DEIA) (GEN-ETI-K; DiaSorin, Italy). Six paraffin-embedded lymph node biopsies from 18 patients as well as 18 samples of peripheral whole blood and 18 sera were investigated. Bartonella henselae DNA was recovered from the whole blood of four patients, and Bartonella henselae and Afipia felis DNA were detected in one patient's lymph node biopsy. This study suggests that PCR-DEIA is sufficiently sensitive to be considered feasible for the molecular diagnosis of cat scratch disease.
- Published
- 2000
- Full Text
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3. Polymerase chain reaction detection of Bartonella henselae bacteraemia in an immunocompetent child with cat-scratch disease.
- Author
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Del Prete R, Fumarola D, Ungari S, Fumarola L, and Miragliotta G
- Subjects
- Antibodies, Bacterial analysis, Cat-Scratch Disease immunology, Child, DNA, Bacterial analysis, Fluorescent Antibody Technique, Direct, Humans, Male, Bacteremia diagnosis, Bartonella Infections diagnosis, Bartonella henselae isolation & purification, Cat-Scratch Disease diagnosis, Polymerase Chain Reaction
- Abstract
Unlabelled: A case of Bartonella henselae bacteraemia is reported in an immunocompetent 8-year-old boy with cat-scratch disease. Serology to B. henselae, diagnosed by polymerase chain reaction, was positive. DNA was extracted from peripheral whole blood and amplified with specific primers targeting the htrA gene of B. henselae. A non-isotopic hybridization assay with a species-specific oligonucleotide probe was used to detect the amplified product., Conclusion: The polymerase chain reaction can be used for the rapid laboratory diagnosis of bacteraemia in cat-scratch disease.
- Published
- 2000
- Full Text
- View/download PDF
4. Molecular epidemiology of KPC-producing Klebsiella pneumoniae from invasive infections in Italy: Increasing diversity with predominance of the ST512 clade II sublineage
- Author
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Conte, Viola, Monaco, Monica, Giani, Tommaso, D'Ancona, Fortunato, Moro, Maria Luisa, Arena, Fabio, D'Andrea, Marco Maria, Rossolini, Gian Maria, Pantosti, Annalisa, Bianchi, E., Catania, M. R., Cavalcanti, P., De Nittis, R., Dusi, P. A., Grandesso, S., Gualdi, P., Imbriani, A., Pini, B., Vincenzi, C., Meledandri, M., Agrappi, C., Parisi, G., Pollini, S., Restelli, A., Rocchetti, A., Vailati, F., Aschbacher, R., Barbaro, A., Bona, R., Chirillo, M., Corradini, S., Cuccurullo, S., De Bernochi, A., Dodi, C., Giammanco, A., Mencacci, Antonella, Milano, F., Miragliotta, G., Mungiguerra, M., Pedna, M. F., Piana, Francesca, Porcheddu, G. M., Rossi, M. R., Santino, I., Sartor, A., Sartore, P., Conte V., Monaco M., Giani T., D'Ancona F., Moro M.L., Arena F., D'Andrea M.M., Rossolini G.M., Pantosti A., Bianchi E., Catania M.R., Cavalcanti P., De Nittis R., Dusi P.A., Grandesso S., Gualdi P., Imbriani A., Pini B., Vincenzi C., Meledandri M., Agrappi C., Parisi G., Pollini S., Restelli A., Rocchetti A., Vailati F., Aschbacher R., Barbaro A., Bona R., Chirillo M., Corradini S., Cuccurullo S., De Bernochi A., Dodi C., Giammanco A., Mencacci A., Milano F., Miragliotta G., Mungiguerra M., Pedna M.F., Piana F., Porcheddu G.M., Rossi M.R., Santino I., Sartor A., Sartore P., Conte, V, Monaco, M, Giani, T, D'Ancona, F, Moro, Ml, Arena, F, D'Andrea, Mm, Rossolini, Gm, Pantosti, A, AR-ISS Study Group on Carbapenemase-Producing K., pneumoniae: Bianchi E, Catania, Mr, Cavalcanti, P, De Nittis, R, Dusi, Pa, Grandesso, S, Gualdi, P, Imbriani, A, Pini, B, Vincenzi, C, Meledandri, Alessio, Agrappi, C, Parisi, G, Pollini, S, Restelli, Ugo, Rocchetti, Romualdo, Vailati, F, Aschbacher, R, Barbaro, A, Bona, R, Chirillo, M, Corradini, S, Cuccurullo, S, De Bernochi, A, Dodi, C, Giammanco, Anna, Mencacci, A, Milano, F, Miragliotta, Giuseppe, Mungiguerra, M, Pedna, Mf, Piana, Daniela, Porcheddu, Gm, Rossi, Mr, Santino, I, Sartor, A, and Sartore, P.
- Subjects
0301 basic medicine ,Microbiology (medical) ,gel ,Klebsiella pneumoniae ,genotype ,030106 microbiology ,Population ,cross-sectional studies ,pulsed-field ,multilocus sequence typing ,electrophoresis, gel, pulsed-field ,humans ,italy ,klebsiella infections ,klebsiella pneumoniae ,molecular epidemiology ,serogroup ,beta-lactamases ,genetic variation ,Settore BIO/19 - Microbiologia Generale ,Cross-Sectional Studies ,Electrophoresis, Gel, Pulsed-Field ,Humans ,Italy ,Klebsiella Infections ,Molecular Epidemiology ,Multilocus Sequence Typing ,Serogroup ,beta-Lactamases ,Genetic Variation ,Genotype ,Pharmacology ,Pharmacology (medical) ,Infectious Diseases ,law.invention ,Microbiology ,beta-Lactamase ,03 medical and health sciences ,law ,Pulsed-field gel electrophoresis ,Typing ,education ,Polymerase chain reaction ,Cross-Sectional Studie ,education.field_of_study ,biology ,Molecular epidemiology ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Settore MED/07 - Microbiologia e Microbiologia Clinica ,bacterial infections and mycoses ,Virology ,electrophoresis ,Multilocus sequence typing ,Human ,Klebsiella Infection - Abstract
Objectives The spread of carbapenem-resistant Enterobacteriaceae (CRE) represents one of the most worrisome problems for clinical medicine worldwide. In Italy, the Antibiotic-Resistance-Istituto Superiore di Sanita surveillance network, in collaboration with the Committee for Antimicrobial Agents of the Italian Society of Clinical Microbiologists, promoted a study to investigate the carbapenem-resistance mechanisms, clonal relatedness and capsular typing of a recent collection of carbapenem-resistant Klebsiella pneumoniae (CR-KP). Methods A total of 17 laboratories distributed across Italy collected all consecutive non-replicate CR-KP isolated from invasive infections during two different study periods (2011-12 and 2013). Carbapenemase genes were searched for by filter hybridization and confirmed by PCR and sequencing. KPC-producing K. pneumoniae (KPC-KP) were typed by PFGE and MLST. Capsular types were identified by wzi gene typing. Results Of the collected K. pneumoniae isolates (n = 461), the overall proportion of CR-KP was 36.2% (n = 167). The majority (97%) of the CR-KP were positive for the blaKPC gene. Among the KPC-KP population, nine different STs were detected with the majority of isolates (94%) belonging to the clonal group (CG) 258. A subpopulation that belonged to ST512 and showed an identical PFGE profile represented the majority (57%) of KPC-KP strains, with a countrywide distribution. Capsular characterization showed the predominance of the wzi154, cps-2 capsular type (88.8% of all CG258 strains). ST258 strains were associated with both cps-1 and cps-2 capsular types, while ST512 was associated with cps-2 only. Conclusions Although a trend to a polyclonal evolution of the Italian KPC-KP was noted, this study showed that the KPC-KP population remained largely oligoclonal with the wide diffusion of an ST512 lineage carrying cps-2 capsular type and producing the KPC-3 enzyme.
- Published
- 2016
5. Monoclonal gammopathy of undetermined significance in pediatric kidney transplant: A possible role of Epstein-Barr virus.
- Author
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Giordano, M., Santangelo, L., Scarasciulli, M. L., Calvario, A., Miragliotta, G., Giordano, P., and Cecinati, V.
- Subjects
EPSTEIN-Barr virus ,CHILDREN'S health ,IMMUNE response ,IMMUNOSUPPRESSIVE agents ,ETIOLOGY of diseases - Abstract
MG is a common event of hematologic malignancies. There are many papers regarding kidney transplantation patients with MGUS in adults, while data in pediatrics are scarce. The etiology and clinical significance of MGUS are unclear both in adults and children. Immunosuppressive drugs, graft antigenicity, and viral infection could play a possible role. The viruses most frequently implicated seem to be EBV or CMV in particular, but their role has to be defined better. However, many investigators have emphasized an impaired balance between an adequate immune response and reactivation of viral infection. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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