1. Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China
- Author
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Wenxu Hong, Chengyao Li, Liang Lu, Jinfeng Zeng, Tong Li, Wen Shao, Tingting Li, Weigang Zhu, and Xianlin Ye
- Subjects
0301 basic medicine ,Adult ,Male ,China ,Hepatitis B virus ,South china ,Occult hepatitis B infection (OBI) ,Genotype ,030106 microbiology ,Blood Donors ,Hepacivirus ,Biology ,Polymerase Chain Reaction ,Virus ,Re-entry policy ,Serology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,0302 clinical medicine ,Humans ,lcsh:RC109-216 ,Serologic Tests ,030212 general & internal medicine ,Hepatitis B Antibodies ,Hepatitis B Surface Antigens ,Potential risk ,Hepatitis B ,Virology ,Occult ,Molecular characterization ,Infectious Diseases ,Parasitology ,Nat ,Mutation ,Non-discriminated reactive (NDR) ,HIV-1 ,Female ,Blood safety ,Research Article - Abstract
Background Blood donor plasma samples were detected by the Ultrio Plus NAT system for HBV, HCV and HIV-1 in Shenzhen blood center, China. Reactive samples underwent further discriminatory testing of a single virus by the same methodology. A large number of cases of non-discriminated reactive (NDR) donors were found, leaving potential risk of transmitting HBV if not deferrals. This study identified those non-discriminated samples. Methods The NDR plasma samples from blood donation screening were detected and classified by additional molecular and serological tests. Molecular characterizations of DNA+ NDR were determined by sequencing analysis. Results A number of 259 (0.21%) NDR plasma samples from screening of 123,280 eligible blood donors were detected, which presented a higher rate (91.1%) of anti-HBc reactivity and nearly half (46.7%) of HBV DNA+ that classified as occult HBV infection (OBI). Most OBI strains were wild-type HBV, but some substitutions V168A, S174 N, V177A, Q129R/L/H, G145A/R in S region of genotype B (OBIB) and T47K/V/A, P49H/L, Q101R/H/K, S174 N, L175S, V177A, T118 M/R/K, G145R/A/K/E, R160K/N in S region of genotype C (OBIC) strains were identified in high frequency. Conclusion Nearly half of NDR blood samples were identified as OBI, in which a number of important mutations were detected. NDR donation might have potential risk for HBV transmission, but need to be further investigated.
- Published
- 2019