1. Fabricating antigen recognition and anti-bioadhesion polymeric surface via a photografting polymerization strategy.
- Author
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Song L, Zhao J, Jin J, Ma J, Liu J, Luan S, and Yin J
- Subjects
- Acrylates chemistry, Adhesiveness, Adsorption, Antibodies metabolism, Enzyme-Linked Immunosorbent Assay, Fibrinogen metabolism, Humans, Immobilized Proteins metabolism, Muramidase metabolism, Phosphorylcholine analogs & derivatives, Phosphorylcholine chemistry, Photoelectron Spectroscopy, Platelet Adhesiveness, Polymethacrylic Acids chemistry, Serum Albumin, Bovine metabolism, Spectroscopy, Fourier Transform Infrared, Antigens metabolism, Light, Polymerization radiation effects, Polymers chemistry
- Abstract
A polymeric platform for immunodiagnostic bioassay was constructed based on biostable polymeric support and two functional monomers, low-fouling methacryloyloxyethyl phosphorylcholine (MPC) and acrylic acid (AAc), by a photografting polymerization approach. Covalent binding of antibody to pAAc chains was achieved by activating carboxyl with NHS/EDC partner. The resultant surface showed obvious suppression of nonspecific protein adsorption and platelet adhesion relative to the control sample, exhibiting good anti-bioadhesion performances. Based on the polymer-supported matrix, a highly sensitive antibody-antigen specific recognition was confirmed in both native plasma and diluted human plasma due to the enhanced antibody loading capacity and lowered bioadhesion as compared to the reference., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
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