1. Electrically stimulated osteogenesis on Ti-PPy/PLGA constructs prepared by laser-assisted processes.
- Author
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Paun IA, Stokker-Cheregi F, Luculescu CR, Acasandrei AM, Ion V, Zamfirescu M, Mustaciosu CC, Mihailescu M, and Dinescu M
- Subjects
- Biocompatible Materials chemistry, Biocompatible Materials pharmacology, Bone and Bones drug effects, Cell Differentiation drug effects, Cell Line, Tumor, Cell Survival drug effects, Electric Stimulation methods, Humans, Laser Therapy methods, Osteoblasts drug effects, Polylactic Acid-Polyglycolic Acid Copolymer, Lactic Acid chemistry, Lactic Acid pharmacology, Osteogenesis drug effects, Polyglycolic Acid chemistry, Polyglycolic Acid pharmacology, Polymers chemistry, Polymers pharmacology, Pyrroles chemistry, Pyrroles pharmacology, Titanium chemistry, Titanium pharmacology
- Abstract
This work describes a versatile laser-based protocol for fabricating micro-patterned, electrically conductive titanium-polypyrrole/poly(lactic-co-glycolic)acid (Ti-PPy/PLGA) constructs for electrically stimulated (ES) osteogenesis. Ti supports were patterned using fs laser ablation in order to create high spatial resolution microstructures meant to provide mechanical resistance and physical cues for cell growth. Matrix Assisted Pulsed Laser Evaporation (MAPLE) was used to coat the patterned Ti supports with PPy/PLGA layers acting as biocompatible surfaces having chemical and electrical properties suitable for cell differentiation and mineralization. In vitro biological assays on osteoblast-like MG63 cells showed that the constructs maintained cell viability without cytotoxicity. At 24 h after cell seeding, electrical stimulation with currents of 200 μA was applied for 4 h. This treatment was shown to promote earlier onset of osteogenesis. More specifically, the alkaline phosphatase activity of the stimulated cultures reached the maximum before that of the non-stimulated ones, i.e. controls, indicating faster cell differentiation. Moreover, mineralization was found to occur at an earlier stage in the stimulated cultures, as compared to the controls, starting with Day 6 of cell culture. At later stages, calcium levels in the stimulated cultures were higher than those in control samples by about 70%, with Ca/P ratios similar to those of natural bone. In all, the laser-based protocol emerges as an efficient alternative to existing fabrication technologies., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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