1. Sequence-encoded bioactive protein-multiblock polymer conjugates via quantitative one-pot iterative living polymerization.
- Author
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Li Z, Song K, Chen Y, Huang Q, You L, Yu L, Chen B, Yuan Z, Xu Y, Su Y, Da L, Zhu X, and Dong R
- Subjects
- Animals, Mice, Tissue Distribution, Proteins chemistry, Humans, Polymerization, Polymers chemistry, Molecular Dynamics Simulation
- Abstract
Protein therapeutics are essential in treating various diseases, but their inherent biological instability and short circulatory half-lives in vivo pose challenges. Herein, a quantitative one-pot iterative living polymerization technique is reported towards precision control over the molecular structure and monomer sequence of protein-polymer conjugates, aiming to maximize physicochemical properties and biological functions of proteins. Using this quantitative one-pot iterative living polymerization technique, we successfully develop a series of sequence-controlled protein-multiblock polymer conjugates, enhancing their biostability, pharmacokinetics, cellular uptake, and in vivo biodistribution. All-atom molecular dynamics simulations are performed to disclose the definite sequence-function relationship of the bioconjugates, further demonstrating their sequence-encoded cellular uptake behavior and in vivo biodistribution in mice. Overall, this work provides a robust approach for creating precision protein-polymer conjugates with defined sequences and advanced functions as a promising candidate in disease treatment., (© 2024. The Author(s).)
- Published
- 2024
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