1. RNA-seq analysis of green tea polyphenols modulation of differently expressed genes in Enterococcus faecalis under low pH.
- Author
-
Yan R, Zhou H, Zheng X, and Zhang X
- Subjects
- Antioxidants pharmacology, Guanosine Triphosphate metabolism, Hydrogen-Ion Concentration, Sequence Analysis, RNA, Tea chemistry, Tea metabolism, Enterococcus faecalis genetics, Polyphenols pharmacology
- Abstract
Enterococcus faecasslis (E. faecalis) is a resident bacterium in the host. The increase in internal stress like low pH may affect the biological effects of E. faecalis. The prebiotic-like function of tea polyphenols can enhance the beneficial effects of its tolerance to environmental stress. In this study, RNA-sequence analysis was used to explore the protective effect of green tea polyphenols (GTP) on E. faecalis under low pH stress. A total of 28 genes were found to be responsive to GTP under low pH stress, including 16 up-regulated and 12 down-regulated. GTP intervention can partly relieve some undesired negative influences, such as the down-regulation of the base excision repair gene and amino acid transport and metabolism gene. The significantly changes were associated with selenocompound metabolism and aminoacyl-tRNA biosynthesis after the intervention of GTP. The present study provided new insights into the growth and continuous adaptation of E. faecalis under stress., (© 2022 The Society for Applied Microbiology.)
- Published
- 2022
- Full Text
- View/download PDF