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Highlights: [•] It was isolated and purified by columns of DEAE-Sepharose F. F. and Sephacryl S H.R. [•] It was a homogeneous polysaccharide and the molecular weight was estimated to be 1.08×104 Da by HPLC. [•] The composition of it was determined as l-fucose, d-mannose, d-glucose and d-galactose by GC analysis. [•] Its structure was determined by MS, along with 1H, 13C and 2D NMR spectroscopy. [ABSTRACT FROM AUTHOR]

Highlights: [•] We purified a novel water-soluble polysaccharide from Wedelia prostrata called WPP60A. [•] Homogeneity and molecular weight (2.40×103 Da) of WPP60A were determined by HPLC. [•] Its structure was established by GC–MS, 1H, 13C, and 2D NMR analysis. [•] The repeating unit consists of [β-d-Fruf-(6→2)-β-d-Fruf-]. [ABSTRACT FROM AUTHOR]

Abstract: A novel water-soluble heteropolysaccharide (PEPS1), with a molecular weight of 1.88×104 Da as determined by high performance liquid chromatography (HPLC), specifically size exclusion chromatography, was isolated from the fruiting bodies of Pleurotus eryngii by hot water extraction and further purification by DEAE Sepharose Fast Flow and Sephacryl S-300 and S-100 High-Resolution chromatography. By use of compositional analysis, methylation analysis, together with 1H, 13C NMR and 2D NMR spectroscopy including COSY, TOCSY, HMQC, HMBC and NOESY experiments, the PEPS1 was identified as a heteropolysaccharide that consists of a α-d-(1→6)-Galactopyranan backbone with a β-d-Mannosyl unit on O-2 of the 2,6-di-O-substituted-d-Galactosyl units, α-(1→6)-3-O-Me-d-Galactopyranan backbone with a terminal α-3-O-Me-d-Galactosyl unit and it also contained a minor 2,3,6-α-d-Galatopyranan units and 4-β-d-Mannopyranan residues. [Copyright &y& Elsevier]

Abstract: A novel water-soluble polysaccharide named PGPB-1, with a molecular weight of 1.3×104 Da as determined by high-performance liquid chromatography (HPLC), was isolated from the fruiting bodies of Pleurotus geesteranus by boiling water as well as DEAE-Sepharose Fast Flow and High-Resolution Sephacryl S-300. On the bases of compositional analysis, methylation analysis and NMR spectroscopy (1H NMR, 13C NMR, 2D-COSY, TOCSY, HMQC, HMBC and NOESY spectra) analysis, the structural elucidation of PGPB-1 consisted of a α-d-(1→6)-galactopyranan and a α-d-3-O-Me-d-galactosyl unit backbone with a α-d-Mannosyl unit on O-2 of the 2,6-di-O-substituted-d-galactosyl units. The repeating unit of the polysaccharide was established as: Display Omitted PGPB-1 also contains a minor proportion of 6-linked-d-Glcp. [Copyright &y& Elsevier]