1. A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier.
- Author
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Zheng B, Ying M, Xie J, Chen Y, Wang Y, Ding X, Hong J, Liao W, and Yu Q
- Subjects
- Animals, Autophagy-Related Protein 5 metabolism, Beclin-1 metabolism, Caspase 3 metabolism, Caspase 9 metabolism, Colitis, Ulcerative chemically induced, Colon pathology, Interleukin-10 metabolism, Male, Mice, Mice, Inbred C57BL, Polysaccharides metabolism, Proto-Oncogene Proteins c-bcl-2 metabolism, Apoptosis drug effects, Autophagy drug effects, Autophagy-Related Protein 7 metabolism, Colitis, Ulcerative drug therapy, Ganoderma metabolism, Polysaccharides pharmacology, Protective Agents pharmacology
- Abstract
Polysaccharides are one of the main active substances in Ganoderma atrum (G. atrum). The purpose of this study was to explore the protective effect of a G. atrum polysaccharide (PSG-1) on DSS-induced colitis and the underlying mechanism. The results showed that PSG-1 could maintain the integrity of the intestinal structure by promoting the expression of goblet cells and levels of tight junction proteins in the colon of DSS-induced colitis mice. Furthermore, PSG-1 relieved the inhibition of Bcl-2 and the overexpression of caspase-3 and caspase-9 caused by DSS. Simultaneously, PSG-1 restored the expression of Atg5, Atg7 and beclin-1 and inhibited the p-akt and p-mTOR levels, suggesting that PSG-1 promoted autophagy via the Akt/mTOR pathway. Moreover, PSG-1 inhibited the content of DCs in the colon and modulated the expression of IL-10 in DCs. In conclusion, PSG-1 alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier.
- Published
- 2020
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