1. Recombinant mussel protein Pvfp5β enhances cell adhesion of poly(vinyl alcohol)/k-carrageenan hydrogel scaffolds.
- Author
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Muscolino E, Costa MA, Sabatino MA, Alessi S, Bulone D, San Biagio PL, Passantino R, Giacomazza D, and Dispenza C
- Subjects
- Animals, Carrageenan metabolism, Cell Adhesion physiology, Hydrogels metabolism, Hydrogels pharmacology, Mice, Recombinant Proteins metabolism, Tissue Engineering methods, Tissue Scaffolds, Fibroblasts metabolism, Polyvinyl Alcohol metabolism
- Abstract
Polymeric hydrogels are increasingly considered as scaffolds for tissue engineering due to their extraordinary resemblance with the extracellular matrix (ECM) of many tissues. As cell adhesion is a key factor in regulating important cell functions, hydrogel scaffolds are often functionalized or loaded with a variety of bioactive molecules that can promote adhesion. Interesting biomimetic approaches exploit the properties of mussel-inspired recombinant adhesive proteins. In this work, we prepared hydrogel scaffolds with a 50%w mixture of k-carrageenan (kC) and polyvinyl alcohol (PVA), by a two-step physical gelation process, and we coated them with Perna viridis foot protein-5β (Pvfp5β). The mechanical and morphological properties of hydrogels were investigated both after conditioning with typical cell culture media and also after coating with the Pvfp5β. The protein resulted strongly adsorbed onto the surface of the hydrogel and also able to penetrate in its interiors to a certain depth, mainly interacting with the kC component of the scaffold as resulted from the confocal analysis. Mouse embryonic fibroblasts NIH-3T3 were seeded on top of the hydrogels and cultured up to two weeks. The role of Pvfp5β in promoting cell adhesion, spreading and colonization of the scaffold was demonstrated., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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