4 results on '"Son, Kyu-Yeol"'
Search Results
2. Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses.
- Author
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Jeong, Young-Ju, Matthijnssens, Jelle, Kim, Deok-Song, Kim, Ji-Yun, Alfajaro, Mia Madel, Park, Jun-Gyu, Hosmillo, Myra, Son, Kyu-Yeol, Soliman, Mahmoud, Baek, Yeong-Bin, Kwon, Joseph, Choi, Jong-Soon, Kang, Mun-Il, and Cho, Kyoung-Oh
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ROTAVIRUSES , *PATHOGENIC microorganisms , *SWINE industry , *EPIDEMIOLOGY , *DATA analysis , *NUCLEOTIDE analysis - Abstract
Porcine group C rotaviruses (RVCs) are considered important pathogens due to their economic impact on pig industry and may also cross the host species barrier toward humans. Unlike RVA, however, genetic and phylogenetic data on RVCs from pigs and other host species are scarce. In the present study, full-length ORF sequences of 26 VP7, 9 VP4 and 9 VP6 genes of Korean porcine RVC strains were compared with those of other known RVC strains by phylogenetic analyses and pairwise identity frequency graphs. Applying the established 85% nucleotide identity cut-off value for RVC VP7 classification, the 26 Korean porcine RVC strains belonged to the G1, G3, G6 and G7 genotypes. Although more complete RVC VP4 sequences are warranted before a definitive cut-off value could be determined, a provisional 83% nucleotide cut-off value proposed for RVC VP4 classification resulted in 7 P-genotypes, 5 of which possessed porcine RVC strains. A 90% nucleotide cut-off value for VP6 divided RVC strains into 7 I-genotypes, 5 of which had porcine RVC strains. G/P/I-genotype comparisons suggested the occurrence of rather frequent reassortment events among Korean porcine RVC strains, and strong geographical differences in the distribution of RVC G-genotypes worldwide. Our data indicate that a large genetic diversity exists among porcine RVC strains. For the final genotype determination of each gene segment, more intensified epidemiological studies on animal and human RVC strains throughout the world are needed. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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3. Full-length genomic analysis of porcine G9P[23] and G9P[7] rotavirus strains isolated from pigs with diarrhea in South Korea
- Author
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Kim, Ha-Hyun, Matthijnssens, Jelle, Kim, Hyun-Jeong, Kwon, Hyung-Jun, Park, Jun-Gyu, Son, Kyu-Yeol, Ryu, Eun-Hye, Kim, Deok-Song, Lee, Woo Song, Kang, Mun-Il, Yang, Dong-Kun, Hyun, Bang-Hun, Park, Sang-Ik, Park, Su-Jin, and Cho, Kyoung-Oh
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DIARRHEA , *GENOMICS , *LABORATORY swine , *ROTAVIRUS diseases , *PHYLOGENY , *EPIDEMIOLOGY , *ZOOLOGICAL microtechnique , *SWINE - Abstract
Abstract: Group A rotaviruses (RVAs) are agents causing severe gastroenteritis in infants and young animals. G9 RVA strains are believed to have originated from pigs. However, this genotype has emerged as the fifth major human RVA genotype worldwide. To better understand the relationship between human and porcine RVA strains, complete RVA genome data are needed. For human RVA strains, the number of complete genome data have grown exponentially. However, there is still a lack of complete genome data on porcine RVA strains. Recently, G9 RVA strains have been identified as the third most important genotype in diarrheic pigs in South Korea in combinations with P[7] and P[23]. This study is the first report on complete genome analyses of 1 G9P[7] and 3 G9P[23] porcine RVA strains, resulting in the following genotype constellation: G9–P[7]/P[23]–I5–R1–C1–M1–A8–N1–T1–E1–H1. By comparisons of these genotype constellations, it was revealed that the Korean G9P[7] and G9P[23] RVA strains possessed a typical porcine RVA backbone, similar to other known porcine RVA strains. However, detailed phylogenetic analyses revealed the presence of intra-genotype reassortments among porcine RVA strains in South Korea. Thus, our data provide genetic information of G9 RVA strains increasingly detected in both humans and pigs, and will help to establish the role of pigs as a source or reservoir for novel human RVA strains. [Copyright &y& Elsevier]
- Published
- 2012
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4. Detection and molecular chracterization of porcine type 3 orthoreoviruses circulating in South Korea
- Author
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Kwon, Hyung-Jun, Kim, Ha-Hyun, Kim, Hyun-Jeong, Park, Jun-Gyu, Son, Kyu-Yeol, Jung, Juyeon, Lee, Woo Song, Cho, Kyoung-Oh, Park, Su-Jin, and Kang, Mun-Il
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ORTHOREOVIRUSES , *MOLECULAR microbiology , *VIRUS diseases in swine , *REVERSE transcriptase polymerase chain reaction , *DNA primers , *CELLULAR inclusions , *BLOOD agglutination , *NUCLEOTIDE sequence - Abstract
Abstract: Orthoreoviruses infect virtually all mammalian species, causing systemic infections including mild gastrointestinal and respiratory illnesses. However, little is known about the prevalence or genetic diversity of porcine orthoreoviruses in South Korea. We examined 237 diarrheic fecal samples collected from 78 pig farms around the country. RT-PCR utilizing primers specific for the L1 gene of mammalian orthoreoviruses showed that 45 (19.0%) samples were positive. The 10 strains isolated from orthoreovirus-positive samples formed typical perinuclear cytoplasmic inclusion bodies and had an atypical hemagglutination pattern; these are characteristics of type 3 orthoreovirus. Phylogenetic analysis of the S1 gene in these 10 Korean and other strains showed that type 3 orthoreoviruses could be divided into four lineages; the 10 Korean strains were included in porcine lineage IV, along with T3/porcine/Sichuan/2006. Sequence analysis showed that strains in lineage IV had nucleotide identities of 97.0–98.1% and deduced amino acid identities of 96.4–98.2%. Sequence analysis of the σ1 protein, a viral attachment protein, revealed that the amino acid sequences associated with neurotropism (amino acids 198–204, 249I, 350D, and 419E) were highly conserved among the Korean strains, confirming that neural tropism was present. In conclusion, our findings suggest that porcine orthoreovirus infections are endemic in pig farms in South Korea and that the 10 novel Korean porcine orthoreoviruses belong to porcine lineage IV of type 3 orthoreovirus. In addition, sequence analysis of S1 genes encoding the σ1 protein showed that the 9 of 10 Korean porcine orthoreoviruses exhibited neural tropism. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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