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Primenom metode PCR u ispitanim uzorcima svinja ustanovljeno je prisustvo virusa Aujeckijeve bolesti, parvovirusa svinja i svinjskog cirkovirusa tip 2. Metodom direktnog sekvenciranja po Sanger-u određen je redosled nukleotida dela gB gena virusa Aujeckijeve bolesti. Tri nukleotidne sekvence virusa Aujeckijeve bolesti uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa sekvencama navedenog virusa izolovanim u u Mađarskoj, Grčkoj i SAD. Na osnovu rezultata filogenetske analize ustanovljeno je da sva tri virusa Aujeckijeve bolesti identifikovana kod svinja u Crnoj Gori pripadaju genotipu I navedenog virusa. Metodom direktnog sekvenciranja po Sanger-u određen je redosled pet odabranih parvovirusa svinja identifikovana u Crnoj Gori. Na osnovu rezultata filogenetske analize parvovirusi svinja identifikovani u Crnoj Gori grupisani su zajedno sa sojevima navedenog virusa poreklom Velike Britanije, Kine, Brazila i SAD. Dobijeni rezultati filogenetske analize su pokazali da svinjski parvovirusi poreklom od svinja sa teritorije Crne Gore pripadaju klasteru E navedenog virusa. Primenom metode direktnog sekvenciranja po Sanger-u određen je redosled nukleotida dela ORF1 regiona genoma virusa PCV2. Nukleotidne sekvence identifikovanih PCV2 virusa imale su visok stepen sličnosti sa sojevima navedenog virusa izolovanim u Italiji, Austriji, Srbiji, Nemačkoj i Kini. Dobijeni rezultati filogenetske analize šest identifikovanih virusa PCV2 su potvrdili da njih pet pripada genotipu PCV2b, a jedan genotipu PCV2c. The presence of Aujeszky's disease virus, porcine parvovirus and porcine circovirus type 2 were detected by PCR method. Nucleotide sequences of Aujeszky's disease virus identified in swine in Montenegro were determined by direct sequencing method according to Sanger. Three nucelotide sequences included in phylogenetic analysis were highly similar with nucleotide sequences of Aujeszky's disease virus strains identified in Hungary, Greece and USA. According to results of phylogenetic analyisis, it was concluded that all three viruses belongs to genotype I. Nucleotide sequences of five porcine parvoviruses identified in Montenegro were determined by direct sequencing method according to Sanger and were highly similar with nucleotide sequences of porcine parvovirus strains isolated in United Kingdom, China, Brasil and USA. Phylogenetic analysis of five porcine parvoviruses revealed that all of them belongs to cluster E. The nucleotide sequences of six porcine circovirus type 2 identified in Montenegro were determined by direct sequencing method according to Sanger and were highly similar with nucleotide sequences of PCV2 strains isolated in Italy, Austria, Serbia, Germany and China. The phylogenetic analysis of six porcine circovirus type 2 showed that five of them belongs to genotype PCV2b, and one to genotype PCV2c.

Prisustvo svinjskog cirkovirusa 2 i parvovirusa svinja ispitano je u osamdeset zbirnih uzoraka (slezina, limfni čvorovi, pluća) poreklom od nevakcinisanih svinja primenom lančane reakcije polimeraze. Sve životinje su bile iz ekstenzivnog načina gajenja i iz različitih regiona Republike Srpske, BiH. Četiri uzorka limfnih čvorova i dva uzorka slezine su bili pozitivni na prisustvo DNK svinjskog cirkovirusa 2 (7,5%), dok je kod pet uzoraka limfnih čvorova utvrđeno prisustvo DNK parvovirusa svinja (6,25%). U uzorcima poreklom od tri svinje utvrđeno je prisustvo nukleinske kiseline oba prethodno navedena virusa (3,75%). Metodom sekvenciranja određena je nukleotidna sekvenca dela ORF1 regiona genoma svih izolata svinjskog cirkovirusa 2 i dela VP2 gena identifikovanih sojeva parvovirusa svinja. Nukleotidne sekvence virusa PCV2 utvrđene u uzorcima svinja poreklom iz RSBiH uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama soja Mantova izolovanog kod svinja u Italiji, zatim sojeva DE00614 i DE22213 izolovanih kod svinja u Nemačkoj kao i sa sojem Jvnan izolovanog kod svinja u Kini. Istovremeno, virusi PCV2 utvrđeni kod svinja u RSBiH su bili delimično slični sa sojem NIVC SRB virusa PCV2 izolovanim kod svinja u Srbiji. Nukleotidnesekvence svinjskih parvovirusa identifikovanih kod svinja u Republici Srpskoj uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa sojem analognim sekvencama sojeva Challenge izolovanim kod svinja u UK, Kresse izolovanim kod svinja u SADu kao i sa sojevima 77 i LZ izolovanim kod svinja u Kini... The presence of porcine circovirus 2 and porcine parvovirus was examined in eighty clinical samples of spleen, lymph nodes and lungs originating from nonvaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (7.5%), while porcine parvovirus DNA was identified in five lymph node samples (6.25%). The presence of both viruses was detected in three lymph node samples (3.75%). Partial nucleotide sequence of ORF1 gene of porcine circovirus 2 and VP2 gene of porcine parvovirus isolates was determined. The nucleotide sequences of identified PCV2 viruses from RSBIH included in phylogenetic typing are similar and cluster together with strain Mantova isolated from domestic pigs in Italy, strains DE00614 and DE22213 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar with the strain NIVC SRB isolated from pigs in Serbia. The nucleotide sequences of identified PPV viruses that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.