1. N-(2- 18 F-fluoropropionyl)-l-glutamate as a potential oncology tracer for PET imaging of glioma.
- Author
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Sun A, Liu S, Tang X, Pan Q, Zhang Z, Ma H, Nie D, Tang C, and Tang G
- Subjects
- Animals, Cell Line, Tumor, Fluorescent Antibody Technique, Glutamates pharmacokinetics, Heterografts, Magnetic Resonance Imaging methods, Rats, Rats, Sprague-Dawley, Brain diagnostic imaging, Fluorine Radioisotopes chemistry, Glioma diagnostic imaging, Glutamates chemistry, Positron-Emission Tomography methods, Radiopharmaceuticals chemistry
- Abstract
N-(2-
18 F-fluoropropionyl)-l-glutamate (18 F-FPGLU), a new N-substituted18 F-labeling l-glutamate, is a potential amino acid tracer for oncology PET imaging with good tumor-to-background contrast in several tumor-bearing mice. Herein, we evaluated the potential value of18 F-FPGLU for PET imaging of glioma in orthotopic glioma-bearing SD rats. A series of competitive inhibition experiments with various types of inhibitors were conducted with C6 cells to investigate the transport mechanism of18 F-FPGLU in glioma. Establishment of orthotopic rat C6 glioma-bearing SD rats models was confirmed by MRI. Then PET imaging of18 F-FPGLU was performed on the orthotopic C6 glioma-bearing SD rats and compared with that of18 F-FDG. After the rats sacrificed, the whole brain was collected and immunofluorescence staining of glial fibrillary acidic protein (GFAP) and matrix metalloproteinase 2 (MMP2) were processed. Na+ -dependent system XAG - and Na+ -independent system XC - are the mainly transporters of18 F-FPGLU in C6 cells. N-methyl-d-aspartate (NMDA) receptor, which is associated with the invasiveness and proliferation of glioma cells, is also involved in the uptake of18 F-FPGLU. High uptake and retention of18 F-FPGLU was observerd in orthotopic glioma with good visualization and the tumor/background ratio reached 2.35 at 60 min post-injection, which was significantly higher than that of18 F-FDG (1.72) in small-animal PET images. High expression of MMP-2 and GFAP was observed in the immunofluorescence staining of glioma xerography slices.18 F-FPGLU seems to be a better potential PET tracer than18 F-FDG for brain glioma imaging with good visualization and ability to assess the tumor activity., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2021
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