1. Comparative [ 18 F]FDG and [ 18 F]DPA714 PET imaging and time-dependent changes of brown adipose tissue in tumour-bearing mice.
- Author
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Niu N, Xing H, Wang X, Ding J, Hao Z, Ren C, Ba J, Zheng L, Fu C, Zhao H, and Huo L
- Subjects
- Animals, Biomarkers, Cell Line, Tumor, Disease Models, Animal, Energy Metabolism, Heterografts, Humans, Mice, Molecular Imaging methods, Neoplasms diagnostic imaging, Neoplasms metabolism, Neoplasms pathology, Adipose Tissue, Brown diagnostic imaging, Adipose Tissue, Brown metabolism, Fluorodeoxyglucose F18, Positron-Emission Tomography methods, Pyrazoles, Pyrimidines
- Abstract
Brown adipose tissue (BAT) is important in monitoring energy homeostasis and cancer cachexia. Different from white adipose tissue, BAT is characterized by the presence of a large number of mitochondria in adipocytes. Translocator protein 18 kDa (TSPO), a critical transporter, is expressed in the outer membrane of mitochondria. We speculated that [
18 F]DPA714, a specific TSPO tracer, may monitor BAT activity in tumor-bearing mice in vivo. We first analyzed the radioactive uptake of positron emission tomography (PET) tracers in BAT of CT26 xenograft mice with 18F-fluorodeoxyglucose ([18 F]FDG) and [18 F]DPA714. We also studied the BAT uptake of [18 F]DPA714 in CT26, A549 and LLC tumor models. The dynamic distribution of [18 F]FDG is quite variable among animals, even in mice of the same tumor model (%ID/g-mean: mean ± SDM, 8.61 ± 8.90, n = 16). Contrarily, [18 F]DPA714 produced high-quality and stable BAT imaging in different tumor models and different animals of the same model. Interestingly, %ID/g-mean of [18 F]DPA714 in BAT was significantly higher on day 26 than that on day 7 in CT26 xenograft model. Taken together, these results strongly indicate the potential feasibility of [18 F]DPA714 PET imaging in investigating BAT and energy metabolism during tumor progression in preclinical and clinical study.- Published
- 2020
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