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Istraživanja su provedena tijekom 2012. i 2013. godine na dva tipa tla: humoglej i močvarno glejno djelomično hidromeliorirano tlo. Korišten hibrid u sjetvi bila je Colonia KWS (KWS). U pokusu je korišten mikrobiološki pripravak Mikro – Vital koji u svom sastavu sadrži bakterijske vrste Azotobacter vinelandii, Azospirillum lipoferum, A. brasilense, Bacillus megaterium, B. subtilis, B. cirkulans, B. polymiksa i Pseudomonas fluorescens. Dvofaktorijalni pokus postavljen je po randomiziranom blok rasporedu u 4 ponavljanja i 6 varijanti: A1 – kontrola ; A2 – primjena mikrobiološkog pripravka Mikro – Vital (1 l ha-1) ; B1 – gnojidba dušikom na osnovi analiza tla ; B2 – reducirana gnojidba dušikom za 20% ; B3 – reducirana gnojidba dušikom za 40%. Najbolji rezultati dobiveni su uz primjenu mikrobiološkog pripravka Mikro – Vital, dok je gnojidba dušikom ovisila o kemijskim svojstvima tla, kao i o vremenskim prilikama tijekom vegetacije.// Investigations were carried out in 2012 and 2013 on two soil types Humogley and Swampy gleys partially hydroameliorated soil. Hybrid Colonia KWS (KWS) was used in sowing .In experiment we used microbial preparation Mikro – Vital which in its composition contains Azotobacter vinelandii, Azospirillum lipoferum, A. brasilense, Bacillus megaterium, B. subtilis, B. cirkulans, B. polymyxa and P. fluorescens. The experiment was set up in completely randomized block design, with 6 different variants and 4 repetitions: A1 – control ; A2 – application of microbiological preparation Mikro – Vital (1 lha-1) ; B1 – mineral nitrogen fertilization on the basis of analysis of the soil ; B2 – reduced nitrogen mineral fertilization by 20% ; B3 – reduced nitrogen mineral fertilization by 40%. The best results were obtained with the application of microbiological preparation Mikro - Vital, while nitrogen fertilization depended on the chemical properties of soil and weather conditions during the growing season.

Dvogodišnji pokusi provedeni su na dva tipa tla (humoglej, eutrično smeđe tlo) tijekom 2012. i 2013. godine. Istraživan je utjecaj simbiotskih, asocijativnih i slobodnih nitrofiksirajućih bakterija na prinos i kvalitetu zrna soje sorte Ika. Pokus je postavljen po randomiziranom blok rasporedu u 4 ponavljanja i 6 varijanti (A1 – kontrola ; A2 – sjeme tretirano bakterijom B. japonicum ; A3 – sjeme tretirano bakterijama B. japonicum, A. brasilense i A. chroococcum ; B1 – mineralna gnojidba dušikom na osnovi analiza tla: B2 – reducirana mineralna gnojidba za 30% ; B3 – reducirana mineralna gnojidba za 60%). Najbolji rezultati na humogleju ostvareni su varijantom A3B3, a na eutričnom smeđem tlu varijantom A3B2.// The two-year experiments were conducted on two types of soil (humogley, eutric brown soil) during the 2012th and 2013th year. The effects of symbiotic, associative and free nitro-fixing bacteria on yield and quality of soybean cultivar Ika were investigated. The experiment was set up by randomized block design in 4 repetitions and 6 different variants, as follows: A1 - control ; A2 - seed treated with the bacteria B. japonicum ; A3 - seed treated with bacteria B. japonicum, A. brasilense and A. chroococcum ; B1 - mineral nitrogen fertilization on the basis of soil analysis ; B2 - mineral fertilization reduced by 30% ; B3 - mineral fertilization reduced by 60%. The best results were achieved on the humogley variant A3B3, and the eutric brown soil variant A3B2.