6 results on '"Wang, Kaijing"'
Search Results
2. TIMP1 Is A Potential Key Gene Associated With The Pathogenesis And Prognosis Of Ulcerative Colitis-Associated Colorectal Cancer
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Huang, Ru, Wang, Kaijing, Gao, Lei, and Gao, Wei
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high-throughput screening assays ,colitis ulcerative ,prognosis ,colorectal neoplasms ,OncoTargets and Therapy ,Original Research - Abstract
Ru Huang,1,* Kaijing Wang,2,* Lei Gao,1 Wei Gao2 1Department of Heart Failure, Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China; 2Department of Colorectal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei GaoDepartment of Colorectal Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of ChinaEmail yoursgaowei@163.comLei GaoDepartment of Heart Failure, Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of ChinaEmail gldlykdx@163.comPurpose: Colorectal cancer (CRC) is the third most frequently diagnosed cancer worldwide. As a high-risk factor for CRC, ulcerative colitis (UC) has been demonstrated to lead to epithelial dysplasia, DNA damage, and eventually cancer. There are approximately 18% of patients with UC may develop CRC.Patients and methods: The gene expression profiles were retrieved from the Gene Expression Omnibus. The Database for Annotation, Visualization and Integrated Discovery was employed to conduct gene annotations. Protein-protein interaction network was constructed by the Search Tool for the Retrieval of Interacting Genes, and further analysed by the Molecular Complex Detection. The correlation between TIMP1 and prognosis was evaluated by the Gene Expression Proï¬ling Interactive Analysis. To predict the potential functions of TIMP1, the GeneMANIA, Coremine, and FunRich were employed. After transfection with small interfering RNA targeting TIMP1, cell proliferation, migration, and apoptosis were determined by CCK-8, scratch wound, and Annexin V-FITC/PI assays, respectively.Results: TIMP1, consistently overexpressed in the initiation and progression of UC-associated CRC (ucaCRC), was identified to be a potential biomarker for the prognosis of patients with CRC. Experimental results showed knockdown of TIMP1 could increase the migration, while did not affect the proliferation and apoptosis of RKO cells. The role of TIMP1 in the malignant transformation of ucaCRC was confirmed by using the protein/gene interactions and biological process annotation and validated by analysing the transcription factors targeting TIMP1.Conclusion: TIMP1 is consistently upregulated in the pathological process of ucaCRC and can be a potential biomarker for the worse prognosis of CRC.Keywords: colitis ulcerative, colorectal neoplasms, high-throughput screening assays, prognosis
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- 2019
3. Tumor Immune Microenvironment Components and Checkpoint Molecules in Anaplastic Variant of Diffuse Large B-Cell Lymphoma.
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Xu, Tianqi, Chai, Jia, Wang, Kaijing, Jia, Qingge, Liu, Yixiong, Wang, Yingmei, Xu, Junpeng, Yu, Kangjie, Zhao, Danhui, Ma, Jing, Fan, Linni, Yan, Qingguo, Guo, Shuangping, Chen, Gang, Chen, Qiongrong, Xiao, Hualiang, Liu, Fang, Qi, Chubo, Liang, Rong, and Li, Mingyang
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DIFFUSE large B-cell lymphomas ,MYELOID-derived suppressor cells ,TUMOR microenvironment ,REGULATORY T cells ,OVERALL survival ,ANAPLASTIC thyroid cancer - Abstract
Background: Anaplastic diffuse large B-cell lymphoma(A-DLBCL) is a rare morphological subtype characterized by the presence of polygonal, bizarre-shaped tumor cells. Our previous research found that A-DLBCL displays many genetic alterations and biological features that differ greatly from those of ordinary DLBCL. However, the status of tumor immune microenvironment components and checkpoint molecules in A-DLBCL remains unclear. Methods: Thirty A-DLBCL patients were enrolled to study tumor immune microenvironment components and checkpoint molecules and their associations with clinicopathological features and prognosis. Results: Patients with A-DLBCL presented higher expression of PD-L1 (40% vs 10%, P=0.004) than patients with ordinary DLBCL. FISH analysis showed that extra copies of PD-L1 were more frequent in A-DLBCL cases than in ordinary DLBCL cases (23.3% vs 4.0%, P=0.001). The numbers of PD-1
+ TILs (tumor infiltrating lymphocytes) and CD8+ T cells were significantly lower in A-DLBCL versus ordinary DLBCL. In contrast, the numbers of GATA3+ Th2 cells, FOXP3+ Tregs and CD33+ myeloid-derived suppressor cells (MDSCs) were significantly higher in A-DLBCL than in ordinary DLBCL. The associations between clinicopathological features and tumor immune microenvironment cell frequency were analyzed in A-DLBCL patients. Briefly, the number of PD-1+ TILs was lower and the number of CD33+ MDSCs was higher in patients with mutated TP53 compared to those with wild-type TP53. The number of FOXP3+ Tregs was much lower in patients with a noncomplete response (CR) to chemotherapy than in those with a complete response. The number of CD8+ T cells showed a decreasing trend in patients with high International Prognostic Index (IPI) scores and in those with concurrent MYC and BCL2 and/or BCL6 abnormalities. Univariate survival analysis showed that patients with PD-L1+ , mPD-L1+ (PD-L1+ nonmalignant stromal cells) or mPD-L1+ status had a significantly poorer overall survival (OS) than those with PD-L1- status. An increase in the number of CD3+ T cells, FOXP3+ Treg cells and T-bet+ Th1 cells was significantly associated with prolonged OS in patients with A-DLBCL. Conclusion: Our study suggests that A-DLBCL displays a distinct pattern of tumor immune microenvironment components and checkpoint molecules that distinguish it from ordinary DLBCL. The analysis of tumor immune microenvironment components and checkpoint molecules could help in predicting the prognosis of A-DLBCL patients and determining therapeutic strategies targeting the tumor immune microenvironment. [ABSTRACT FROM AUTHOR]- Published
- 2021
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4. High RSK4 expression constitutes a predictor of poor prognosis for patients with clear cell renal carcinoma.
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Ma, Jing, Wang, Kaijing, Chai, Jia, Xu, Tianqi, Wei, Jie, Liu, Yixiong, Wang, Yangang, Xu, Junpeng, Li, Mingyang, and Fan, Linni
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RENAL cell carcinoma , *PROGNOSIS , *SURVIVAL rate , *TREATMENT effectiveness , *HYDRONEPHROSIS - Abstract
This research focuses on exploring RSK4 protein expression within Clear Cell Renal Cell Carcinoma (ccRCC), based on these investigations on level of expressions coupled with the relevance to clinicopathologic features and clinical outcomes. The expression of RSK4 in 48 ccRCC and 20 hydronephrosis samples were under the detection of immunohistochemistry; besides, its relevance to the combination of clinicopathologic features with prognosis was committed in virtue of statistical approaches. The 48 ccRCC samples included 36 (75%, 36/48) positive for RSK4, while the positive rate in hydronephrosis samples were 5 (25%, 5/20). Statistical analysis showed that RSK4 in ccRCC samples express higher expression the hydronephrosis samples (P < 0.05). Furthermore, the expression of RSK4 in ccRCC samples weren't correlated with ages and genders (P > 0.05), while WHO/ISUP nucleolar grade harboured relevance to low survival rate (P = 0.018). Molecular researches demonstrated that over-expression of RSK4 was able to upgrade the proliferation capability of ccRCC cell lines. According to the expression pattern and molecular systems featured RSK4 in ccRCCs, it performed the function of a latent independent prognostic factor performing the function of a newly built latent therapeutic aim oriented with the patients undergoing RCC. Moreover, the specific mechanism of action is expected to be revealed in the future research. [ABSTRACT FROM AUTHOR]
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- 2021
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5. TOPK: A new predictor of the therapeutic response to neoadjuvant chemotherapy and prognosis in triple-negative breast cancer.
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Wang, Kaijing, Chai, Jia, Xu, Junpeng, Wei, Jie, Li, Peifeng, Liu, Yixiong, Ma, Jing, Xu, Tianqi, Zhao, Danhui, Yu, Kangjie, Fan, Linni, Yan, Qingguo, Guo, Shuangping, Li, Mingyang, and Wang, Zhe
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TRIPLE-negative breast cancer , *NEOADJUVANT chemotherapy , *BREAST cancer prognosis , *BREAST cancer , *OVERALL survival , *CANCER cells , *DOCETAXEL - Abstract
Triple-negative breast cancer (TNBC) has a high probability of relapse and poor overall survival. Neoadjuvant chemotherapy (NACT) is currently a routine treatment strategy for TNBC, but some patients do not respond well. T-LAK cell-originated protein kinase (TOPK) is highly expressed in breast cancer cells and contributes to cancer cell proliferation. The present study aimed to investigate the correlation of TOPK expression with NACT treatment response and prognosis in TNBC. We collected 66 pairs of TNBC samples before and after NACT with docetaxel + epirubicin + cyclophosphamide (TEC). The Miller-Payne (MP) system was used to assess the therapeutic response to NACT in TNBC patients. Immunohistochemistry analysis showed that TNBC patients with high TOPK expression before NACT had a poor treatment response and a poor prognosis. The expression of TOPK after NACT was significantly higher than that before NACT in patients with MP grade 1–3. In contrast, patients with MP grade 4–5 had significantly lower TOPK expression after NACT than before NACT, and the expression change in Ki-67 in patients with MP grade 4–5 exhibited the same trend. Survival analysis revealed that patients with TNBC accompanied by elevated TOPK expression before NACT had a worse prognosis than those with lower TOPK expression. TOPK may be a novel predictor for the therapeutic response to NACT and prognosis for patients with TNBC. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Overexpression of OTX1 promotes tumorigenesis in patients with esophageal squamous cell carcinoma.
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Chai, Jia, Xu, Tianqi, Yang, Yanru, Yuan, Yuan, Xu, Junpeng, Liu, Jin, Wang, Kaijing, Lv, Yao, Chai, Jialin, Kang, Yulin, Chen, Ligang, Qin, Junhui, Jia, Qingge, and Li, Mingyang
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SQUAMOUS cell carcinoma , *GENETIC profile , *GENETIC overexpression , *GENE expression , *PROGNOSIS - Abstract
Esophageal squamous cell carcinoma (ESCC) is a multifactorial disease and the sixth leading cause of death from cancer worldwide. Patients with ESCC usually have a short survival period due to the late stage at diagnosis. Incidence rates of ESCC remain high among the elderly. With recent advances, it has been demonstrated that ESCC tumors display a unique genetic profile. This study aimed to examine the possible function of OTX1 in ESCC. We collected paraffin-embedded tumor tissues from 107 patients originally diagnosed with ESCC at Xijing Hospital and fresh-harvested and paired adjacent normal esophageal tissues from 15 ESCC patients undergoing curative resection. The expression level of OTX1 was evaluated through immunohistochemistry and western blot. Prognostic and survival analyses were conducted using univariate/multivariate analysis and log-rank analysis with SPSS 23.0. Cell models and xenograft models were used to examine the overexpression of OTX1 in vivo and in vitro. OTX1 was overexpressed in ESCC tissues compared with normal esophageal tissues. Both the mRNA expression level and protein level of OTX1 were higher than they were in paired normal tissue. Prognostic and OS analyses showed that the OTX1 expression level was an individual prognostic factor in ESCC patients. Cell viability was significantly promoted when OTX1 was overexpressed in ESCC cell, Furthermore, downregulating OTX1 in EC109 cell significantly attenuated the cell proliferation migration and invasion. Flow cytometric detection showed that cells overexpressing OTX1 were predominantly in the S and G2&M phases. In the xenograft model, both tumor size and weight in the OTX1 overexpression group were significantly larger than those in the control group. OTX1 is an independent prognostic factor of ESCC and contributes to tumorigenesis both in vivo and in vitro. [ABSTRACT FROM AUTHOR]
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- 2022
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