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To understand potential pro-oncological effects of lower dose paclitaxel treatment in cervical cancer cells, we investigated the potential roles of KRT17 on migration and proliferation of cervical cancer cells which might respond to cytoskeletal-based drugs treatments. We extracted the clinic data of cervical cancer patients from TCGA database to investigate mRNA expression of different keratins. HPV genotypes were identified by reverse transcription PCR. krt17 mRNA and EMT markers were quantified by real-time PCR. krt17 and EMT markers protein were immunoblotted by western blot. Cell viability was detected by CCK8. Cell migration was performed by transwell migration assay. Our results showed that HPV16 infection correlated with the expression of KRT17 in cervical cancer cell lines. KRT17 knockdown would decrease Snail2 and elevate E-Cadherin to inhibit migration of Caski cells and SiHa cells. Lower dose of paclitaxel promoted SiHa proliferation, it also significantly promoted the migration of Caski cells. Otherwise, colchicine and higher dose of paclitaxel dose-dependently suppressed the proliferation and migration of Caski cells and SiHa cells. Moreover, KRT17 knockdown significantly facilitated cytoskeletal-based drugs to inhibit migration and induce cytotoxicity in cervical cancer cells. KRT17 played pivotal oncogenic roles in cell survival, migration and paclitaxel-induced resistance of cervical cancer cells. Thus, KRT17 would serve as a promising target for compromising paclitaxel-induced resistance and metastasis. KRT17 confers paclitaxel-induced resistance and migration to cervical cancer cells. Tumor tissue is consist of different tumor cells, they show distinct cellular morphology, metastatic, chemosensitivity or other potentials, this is called tumor heterogeneity. In the present study, we found that lower-dose of paclitaxel induced proliferation and paclitaxel-resistance in certain cervical cancer cells, while also promoted migration in the other cervical cancer cells. Interestingly, KRT17 knockdown abolished above effects of paclitaxel, which facilitated cytoskeletal-based drugs to inhibit migration and induce cytotoxicity in these cervical cancer cells. Unlabelled Image [ABSTRACT FROM AUTHOR]