1. Identification and Validation of a PPP1R12A-Related Five-Gene Signature Associated With Metabolism to Predict the Prognosis of Patients With Prostate Cancer.
- Author
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Zou, Zhihao, Liu, Ren, Liang, Yingke, Zhou, Rui, Dai, Qishan, Han, Zhaodong, Jiang, Minyao, Zhuo, Yangjia, Zhang, Yixun, Feng, Yuanfa, Zhu, Xuejin, Cai, Shanghua, Lin, Jundong, Tang, Zhenfeng, Zhong, Weide, and Liang, Yuxiang
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PROSTATE cancer prognosis ,PROSTATE cancer patients ,PROGNOSIS ,PHOSPHOPROTEIN phosphatases ,ANDROGEN receptors ,PROGNOSTIC models - Abstract
Background: Prostate cancer (PCa) is the most common malignant male neoplasm in the American male population. Our prior studies have demonstrated that protein phosphatase 1 regulatory subunit 12A (PPP1R12A) could be an efficient prognostic factor in patients with PCa, promoting further investigation. The present study attempted to construct a gene signature based on PPP1R12A and metabolism-related genes to predict the prognosis of PCa patients. Methods: The mRNA expression profiles of 499 tumor and 52 normal tissues were extracted from The Cancer Genome Atlas (TCGA) database. We selected differentially expressed PPP1R12A-related genes among these mRNAs. Tandem affinity purification-mass spectrometry was used to identify the proteins that directly interact with PPP1R12A. Gene set enrichment analysis (GSEA) was used to extract metabolism-related genes. Univariate Cox regression analysis and a random survival forest algorithm were used to confirm optimal genes to build a prognostic risk model. Results: We identified a five-gene signature (PPP1R12A , PTGS2 , GGCT , AOX1 , and NT5E) that was associated with PPP1R12A and metabolism in PCa, which effectively predicted disease-free survival (DFS) and biochemical relapse-free survival (BRFS). Moreover, the signature was validated by two internal datasets from TCGA and one external dataset from the Gene Expression Omnibus (GEO). Conclusion: The five-gene signature is an effective potential factor to predict the prognosis of PCa, classifying PCa patients into high- and low-risk groups, which might provide potential novel treatment strategies for these patients. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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