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Your search keyword '"femtosecond studies"' showing total 3 results
Start Over Descriptor "femtosecond studies" Topic protein structure
3 results on '"femtosecond studies"'

2. In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells

Author

Arjen J. Jakobi, Daniel M. Passon, Kèvin Knoops, Francesco Stellato, Mengning Liang, Thomas A. White, Thomas Seine, Marc Messerschmidt, Henry N. Chapman, and Matthias Wilmanns

Subjects
protein structure, X-ray crystallography, femtosecond studies, nanocrystals, free-electron laser, Crystallography, QD901-999
Abstract

The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. The observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.

Published
2016
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3. In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells

Author

Marc Messerschmidt, Mengning Liang, Henry N. Chapman, Matthias Wilmanns, Thomas A. White, Francesco Stellato, Daniel M. Passon, Arjen J. Jakobi, Kèvin Knoops, Thomas Seine, and Molecular Cell Biology

Subjects
0301 basic medicine, 02 engineering and technology, SYNCHROTRON-RADIATION, Biochemistry, FEMTOSECOND CRYSTALLOGRAPHY, 03 medical and health sciences, Protein structure, free-electron laser, nanocrystals, Protein purification, Microbody, General Materials Science, ddc:530, MICROBODIES, protein structure, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries), X-ray crystallography, ELECTRON-MICROSCOPY, Crystallography, Chemistry, femtosecond studies, Settore FIS/07, General Chemistry, nanochrystals, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Yeast, Research Letters, Alcohol oxidase, PROTEIN NANOCRYSTALLOGRAPHY, 030104 developmental biology, QD901-999, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, 2-DIMENSIONAL CRYSTALS, METHANOL, 0210 nano-technology, Protein crystallization, GROWN HANSENULA-POLYMORPHA, PEROXISOMES, Powder diffraction
Abstract

The application of serial femtosecond crystallography to naturally occurring peroxisomal protein crystals within yeast cells is described. The concept of utilizing peroxisomes for the production of protein nanocrystals is outlined., The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. The observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.

Published
2016
Full Text
View/download PDF

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