1. Phase separation ability and phosphatase activity of the SHP1-R360E mutant.
- Author
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Zhang Q, Kong W, Zhu T, Zhu G, Zhu J, Kong X, and Du Y
- Subjects
- Cell Differentiation, Protein Tyrosine Phosphatase, Non-Receptor Type 6 genetics, Protein Tyrosine Phosphatase, Non-Receptor Type 6 metabolism, Protein Tyrosine Phosphatase, Non-Receptor Type 11 genetics, Protein Tyrosine Phosphatase, Non-Receptor Type 11 metabolism
- Abstract
SHP1 is a non-receptor protein tyrosine phosphatase that is widely expressed in hematopoietic cells such as white blood cells, neutrophils, and immune cells. SHP1 can regulate the occurrence and differentiation of immune cells and plays an important role as a tumor suppressor. Previous studies have suggested that SHP2, the homologous protein of phosphatase SHP1, can undergo liquid-liquid phase separation (LLPS). Therefore, in this study, we investigated if SHP1 is also capable of LLPS. To the best of our knowledge, our study is the first to reveal that SHP1 has the ability to undergo LLPS. In addition, we identified an important residue, SHP1-R360E, that can completely inhibit the LLPS ability of SHP1, but this mutation has no remarkable effect on SHP1's enzymatic activity. This allows us to explore the phosphatase activity and phase separation ability of SHP1 separately, providing a basis for future exploration of the phase separation mechanism of phosphatases., Competing Interests: Declaration of competing interest The authors have no conflict of interests to declare., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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