1. Granulocyte colony-stimulating factor-induced upregulation of Jak3 transcription during granulocytic differentiation is mediated by the cooperative action of Sp1 and Stat3.
- Author
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Mangan JK, Tantravahi RV, Rane SG, and Reddy EP
- Subjects
- Animals, Cells, Cultured, Electrophoretic Mobility Shift Assay, Janus Kinase 3, Mice, Protein-Tyrosine Kinases metabolism, Up-Regulation, Cell Differentiation, Gene Expression Regulation, Granulocyte Colony-Stimulating Factor pharmacology, Granulocytes cytology, Protein-Tyrosine Kinases genetics, STAT3 Transcription Factor metabolism, Sp1 Transcription Factor metabolism, Transcription, Genetic
- Abstract
We previously demonstrated that Jak3 is a primary response gene for G-CSF and ectopic overexpression of Jak3 can accelerate granulocytic differentiation of normal mouse bone marrow cells induced by G-CSF and GM-CSF. To gain insight into the regulation of G-CSF-induced transcription of Jak3, we constructed deletion and linker scanning mutants of the Jak3 promoter sequences and performed luciferase reporter assays in the murine myeloid cell line 32Dcl3, with and without G-CSF stimulation. These experiments showed that mutation of a -67 to -85 element, which contained a putative Sp1 binding site, or mutation of a -44 to -53 GAS element resulted in a marked reduction of Jak3 promoter activity. Electrophoretic mobility shift assays revealed that Sp1 and Stat3 present in nuclear lysates of 32Dcl3 cells stimulated with G-CSF can bind to the -67 to -85 element and -44 to -53 GAS element, respectively. In addition, cotransfection of a constitutively active mutant of Stat3 along with a Jak3 promoter/luciferase reporter resulted in enhanced Jak3 promoter activity. Together, these results demonstrate that activation of Jak3 transcription during G-CSF- induced granulocytic differentiation is mediated by the combined action of Sp1 and Stat3, a mechanism also shown to be important in IL-6-induced monocytic differentiation.
- Published
- 2006
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