1. c-Myb-mediated inhibition of miR-601 in facilitating malignance of osteosarcoma via augmentation of PKMYT1.
- Author
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Luo P, Fang J, Chen H, He F, Xiao S, Liu H, Zhu S, Luo J, and Jiang C
- Subjects
- Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Computational Biology, Gene Expression Regulation, Neoplastic, Humans, Membrane Proteins metabolism, Prognosis, Bone Neoplasms genetics, Bone Neoplasms pathology, MicroRNAs genetics, MicroRNAs metabolism, Osteosarcoma genetics, Osteosarcoma pathology, Protein Serine-Threonine Kinases genetics, Protein Serine-Threonine Kinases metabolism, Protein-Tyrosine Kinases genetics, Protein-Tyrosine Kinases metabolism, Proto-Oncogene Proteins c-myb genetics, Proto-Oncogene Proteins c-myb metabolism
- Abstract
The crosstalk between osteosarcoma (OS) development and abnormally expressed microRNA (miR)-601 is not explored explicitly. Here, we identified the downregulated miR-601 in osteosarcoma (OS) through a comprehensive bioinformatics analysis of GEO Datasets. The results indicated that miR-601 was downregulated in both OS cells and tissues. The OS patients with reduced expression of miR-601 displayed worse prognosis. The results of in vitro and in vivo assay revealed that elevated miR-601 inhibited the proliferative, migratory and invasive capacities in OS cells. Mechanically, miR-601 exerted its function via targeting oncogene protein kinase membrane associated tyrosine/threonine 1 (PKMYT1) at post-transcriptional level. Moreover, miR-601 was attenuated by c-Myb at transcriptional level. Taken together, our studies reveal that miR-601 is a suppressive gene negatively correlated with malignancy of OS., (© 2022. The Author(s).)
- Published
- 2022
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