1. Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
- Author
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Chen JF, Tsai YT, Lai YH, Lin CC, Chou HC, Kuo WH, Ko ML, Wei YS, Wang YS, Lin MW, Chen YJ, Lee YR, and Chan HL
- Subjects
- Cell Line, Cell Line, Tumor, Cytoskeleton drug effects, Cytoskeleton metabolism, Endoplasmic Reticulum Stress drug effects, Hep G2 Cells, Hepatocytes drug effects, Hepatocytes metabolism, Humans, Liver Neoplasms metabolism, Oxidation-Reduction drug effects, Protein Folding drug effects, Antineoplastic Agents pharmacology, Liver Neoplasms drug therapy, Polyporales chemistry, Proteomics
- Abstract
Antrodia Cinnamomea is a fungus species widely used as a herb medicine for hypertension, cancer and handover. Nevertheless, the biological roles of Antrodia Cinnamomea on the molecular mechanism of liver cancer are not entirely understood. To determine whether Antrodia Cinnamomea is able to be used for the treatment of liver cancer and its molecular mechanism, we examined the effect of Antrodia Cinnamomea on the differential proteomic patterns in liver cancer cell lines HepG2 and C3A as well as in Chang's liver cell, a normal liver cell, using quantitative proteomic approach. The proteomic analysis demonstrated that abundance of 82, 125 and 125 proteins was significantly altered in Chang's liver cells, C3A and HepG2, respectively. The experimental outcomes also demonstrated that Antrodia Cinnamomea-induced cytotoxicity in liver cancer cells mostly involved dysregulation of protein folding, cytoskeleton regulation, redox-regulation, glycolysis pathway as well as transcription regulation. Further analysis also revealed that Antrodia Cinnamomea promoted misfolding of intracellular proteins and dysregulate of cellular redox-balance resulting in ER-stress. To sum up our studies demonstrated that the proteomic strategy used in this study offered a tool to investigate the molecular mechanisms of Antrodia Cinnamomea-induced liver cancer cytotoxicity. The proteomic results might be further evaluated as prospective targets in liver cancer treatment., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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