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Start Over Descriptor "synthetic DNA fragments" Topic prrsv
2 results on '"synthetic DNA fragments"'

1. Rapid reconstruction of porcine reproductive and respiratory syndrome virus using synthetic DNA fragments

Author

Julien Mélade, Géraldine Piorkowski, Hawa Sophia Bouzidi, Alain Medawar, Claudine Raffy, Xavier de Lamballerie, and Antoine Nougairède

Subjects
PRRSV, Reverse genetics, synthetic DNA fragments, ISA, Biotechnology, TP248.13-248.65
Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most challenging infectious disease of pig populations causing devastating economic loss to swine industry. Reverse genetics allow to engineer modified viruses such attenuated strains for vaccine development. Some reverse genetic systems were described for PRRSVs but, due to genome complexity of PRRSVs, construction and modification of such systems remain laborious and time-consuming.In this study, we described a reverse genetics approach based on the “Infectious-Subgenomic Amplicons” (ISA) method to rescue infectious PRRSV particles. Permissive cells were transfected with 4 overlapping synthetic DNA fragments covering the entire genome of PRRSV which allowed the rapid reconstruction of the complete virus genome and the subsequent generation of infectious wild-type particles within days.The ISA method represent a rapid alternative of conventional reverse genetic systems. This method will help to generate genetically modified and attenuated strains for the development of sanitary countermeasures in the future.

Published
2021
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2. Rapid reconstruction of porcine reproductive and respiratory syndrome virus using synthetic DNA fragments

Author

Alain Medawar, Antoine Nougairède, Géraldine Piorkowski, Xavier de Lamballerie, Claudine Raffy, Hawa Sophia Bouzidi, and Julien Mélade

Subjects
Biophysics, ISA, Transfection, synthetic DNA fragments, Biology, Amplicon, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, Biochemistry, Virology, Genome, Reverse genetics, Virus, Computer Science Applications, Genetically modified organism, Structural Biology, Infectious disease (medical specialty), PRRSV, Genetics, TP248.13-248.65, ComputingMethodologies_COMPUTERGRAPHICS, Research Article, Biotechnology
Abstract

Graphical abstract, Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most challenging infectious disease of pig populations causing devastating economic loss to swine industry. Reverse genetics allow to engineer modified viruses such attenuated strains for vaccine development. Some reverse genetic systems were described for PRRSVs but, due to genome complexity of PRRSVs, construction and modification of such systems remain laborious and time-consuming. In this study, we described a reverse genetics approach based on the “Infectious-Subgenomic Amplicons” (ISA) method to rescue infectious PRRSV particles. Permissive cells were transfected with 4 overlapping synthetic DNA fragments covering the entire genome of PRRSV which allowed the rapid reconstruction of the complete virus genome and the subsequent generation of infectious wild-type particles within days. The ISA method represent a rapid alternative of conventional reverse genetic systems. This method will help to generate genetically modified and attenuated strains for the development of sanitary countermeasures in the future.

Published
2021

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