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The aim of this study was the identification of the causative agent of the basal glume rot of wheat Pseudomonas syringae pv. atrofaciens from the affected weeds in wheat crops, and determination of its virulent properties. Isolation of P. syringae pv. atrofaciens from weeds of wheat crops was carried out by classical microbiological methods. To identify isolated bacteria, their morphological, cultural, biochemical, and serological properties as well as fatty acids and Random Amplification of Polymorphic DNA (RAPD)-PCR (Polymerase chain reaction) profiles with the OPA-13 primer were studied. Pathogenic properties were investigated by artificial inoculation of wheat plants and weed plants, from which bacteria were isolated. For the first time, bacteria that are virulent both for weeds and wheat were isolated from weeds growing in wheat crops. It was shown that the fatty acids profiles of the bacteria isolated from the weeds contained typical for P. syringae pv. atrofaciens fatty acids, in particular, hydroxy acids: 3-hydroxydecanoic, 2-hydroxydodecanoic, and 3-hydroxydodecanoic. RAPD-PCR profiles of the newly isolated strains were identical to those of the collection strains P. syringae pv. atrofaciens UCM B-1011 and P. syringae pv. atrofaciens UCM B-1014 and contained a dominant fragment of 700 bp. The isolated strains, according to their phenotypic and genotypic properties, were identified as P. syringae pv. atrofaciens. It was established that the causative agent of basal glume rot of wheat P. syringae pv. atrofaciens is polyphagous and capable of infecting a wide range of plants. The main control measure for cereals diseases caused by P. syringae pv. Atrofaciens—crop rotations with nonhost species, should be revised, and alternative control methods must be proposed. [ABSTRACT FROM AUTHOR]

Electron microscope investigations have revealed disintegration of the cell wall of Pseudomonas syringae pv. atrofaciens and subsequent killing of bacterial cells after application of 1% solutions of the citrates of Ag-Cu nanoparticles, the complex Co-Cu-Zn-Fe-Mn-Mo-Mg (Avatar-1) and iodine-selenium. In field conditions, it was revealed that pre-sowing treatment of wheat seeds with the aforementioned 1% solutions significantly inhibited the development of basal glume rot in cereals with artificial infection of Pseudomonas syringae pv. atrofaciens. Consequently, reduction of the typical symptoms of basal glume rot of cereals and the percentage of isolated cells of Pseudomonas syringae pv. atrofaciens D13 were detected in wheat plants upon pre-sowing treatment of wheat seeds by experimental biocides. These results can be explained by structural changes in the bacterial cells of the pathogen that lead to reduction of viable forms of bacteria due to damage of both the cell wall and internal contents. [ABSTRACT FROM AUTHOR]