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Ho: GdVO4 crystal is the host material for the production of laser working in the middle infrared range. In this contribution, the characteristic parameters of the Ho: GdVO4 crystal were measured, and the material was used as a gain medium to build a diode-pumped laser for the first time, to reach a laser output at 2047.9 nm. The output beam quality factor M2 was measured to be 1.4 and 1.3 in x-direction and y-direction, respectively. In addition, the influence of the transmittance of the output mirror on the generation of laser was obtained through exploration. The results showed that the laser wavelength blue-shifted as the output transmittance increased.