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The purpose of the research is to study of local irritant action of complex insectoacaricide drug "RolfClub 3D shampoo” on rats’ skin and guinea pigs’ mucous membranes of the eyes.Materials and methods. Local irritant action of complex insectoacaricide drug "RolfClub 3D shampoo” was evaluated in the experiment on determination of acute skin toxicity of rats on the thickness of skin fold. Only animals with healthy skin, which was kept on standard dietary and which has undergone 7 days absolute quarantine before experiment, were used. Studies on determination of local irritant action of aqueous solution of active ingredients combination of shampoo on conjunctiva of guinea pigs’ eyes were conducted on adult albino guinea pigs with body weight of 250–300 g. Each animal's eye condition was visually evaluated for determination of expressed injuries not later than 24 hours.Results and discussion. Symptoms of irritant action were not noticed in the case of exposure of aqueous solution of active ingredients combination of shampoo on rats’ skin. After application skin areas was not differ from control skin areas. Redness of whole conjunctiva and sclera was noted in the case of application of solution on mucous membranes of the eyes. This event disappeared in 24 hours. Condition of animals’ mucous membranes of the eyes pretreated water was within the physiological range. Aqueous solution of active ingredients combination has mild effect on mucous membranes of the eyes; recovery up to normal range carries within 24 hours.

The purpose of the research is to study the efficiency of complex medicinal product for veterinary use "RolfClub 3D shampoo” for treatment of dogs’ and cats’ ectoparasitosis.Materials and methods. Cats and dogs affected by fleas Ctenocephalides felis and C. canis, lice Felicola subrostratus and Trichodectes canis, sucking lice Linognathus setosus were selected for the experiment. Presence of specific disease symptoms (pruritus, rumpled and flat coat, scratchings, and places of hair removal) was noted at the time of visual examination of animals’ dermatic hair-coat covering. All infected animals were divided into experimental and control groups. Complex insectoacaricide drug "RolfClub 3D shampoo” ("SPC "Ecoprom") in the form of solution was administered according to the instruction. Before dressing animals’ dermatic hair-coat covering was richly washed by warm water, then the drug was applied at a rate of 1 ml per 1 kg of body weight spreading throughout surface of dermatic hair-coat covering and slightly rubbing till foaming avoiding contact with eyes, ears, and avoiding licking off drug by animals. Shampoo was carefully washed out with warm water in 5–7 minutes. Zoohygienical shampoo was used for the animals from control group. In 24 hours, 2, 5, 10 days shampoo efficiency was evaluated on the basis of calculation of amount of ectoparasites before and after single dressing by the drug "RolfClub 3D shampoo”.Results and discussion. It has been established that the drug "RolfClub 3D shampoo” has significant insecticide effect. Single animals’ dressing by the drug "RolfClub 3D shampoo” provided death of fleas, sucking lice, lice. In 24 hours, 2, 5, 10 days after beginning of experiment all treated animals were free of ectoparasites (fleas, sucking lice, lice). Therapeutic drug efficiency was 100% in the case of all ectoparasites diseases (ctenocephalosis, linognatozis, trichodectozis). Side effects and events were not identified in the case of drug administration.

The purpose of the research is to study the effectiveness of «Neoterica Protecto 12» product as polymer tape to prevent myiasis of dogs andcats. Materials and methods. In course of the experiment, spontaneously infected dogs and cats of different ages and breeds were used. The animals were not treated with anti-parasitic drugs for the previous 30 days; they had not been wearing insecticide collars for three months. The diagnosis was made comprehensively taking into account the epizootological data, clinical features and on the basis of careful examination and detection of ectoparasites and their eggs on the skin-and-coat of animals. The intensity of the invasion of animals was estimated by visual rating of insects on the animal's body parts (abdomen, head, lumbar region, and edge bone, inguinal and perineal zone) with a size of 10 × 10 cm. Results and discussion. The high therapeutic effectiveness of the product was found to be 100% when the animals were infected with Ctenocephalides canis, C. felis, Felicola subrostratus, Trichodectes canis and Linognathus setosus ectoparasites. No animal infected with ectoparasites was found within 28 days of applying the collar. No behavior changes and characteristic symptoms were found when observing the animals; general condition of the animals did not deteriorate. Clinical examination showed no evidence of itching; the restoration of skin and coat where scratches and alopecia were earlier noted. The re-infection of dogs and cats with ectoparasites during the following 6 months is hardly in evidence.

The purpose of the research is to assess the subchronic toxicity of the complex drug for dogs and cats “Inspector Kvadro” for cutaneous use.Materials and methods. The experiment was carried out on 40 male rats with an initial body weight of 220–240 g. The animals were divided into 4 equivalent groups of 10 rats each. The drug was applied daily for 7 days on a clipped skin in the back at doses of 1080; 540 and 216 mg/kg. During the experiment, we observed the general condition and behavior of animals, possible death, intake of food and water, visible physiological functions, the condition of the coat and skin at the site of application of the drug in order to evaluate the irritating effect. On the first day and 10 days after the last use of the drug, the animals were euthanized and blood samples were taken to determine hematological and biochemical parameters, a macroscopic examination of the organs was carried out, and the functional state of the central nervous system was evaluated.Results and discussion. Daily application on the skin of the drug in test doses did not lead to a change in the general condition and behavior of rats. Experienced animals normally took food and water, adequately responded to external stimuli. However, the recorded changes in some indicators indicate damage to the hepatocytes and indicate insufficient liver function. Given the increased relative liver mass of the animals of the first experimental group and the change in biochemical parameters, we can conclude that the drug has a pronounced hepatotoxic effect at a dose of 1080 mg/kg for cutaneous use for 7 days. Accordingly, the dose of 1080 mg/kg is toxic, the doses of 540 and 216 mg/kg are threshold.