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Burkholderia vietnamiensis is a diazotroph capable of producing siderophores, as well as colonizing the rhizosphere and endosphere of rice. Although its taxonomy is not completely resolved, more isolations are needed to clarify the phylogenetic relationships with other species. The present work sought to implement a collection of phytobeneficial Burkholderia strains associated with different rice cultivars in the Peruvian jungle in order to carry out comparative genomics studies. 211 root endophytic bacteria were isolated and selected according to their ability to fix atmospheric nitrogen and production of siderophores, subsequently, molecular discrimination was carried out by amplifying the recA housekeeping gene. Only 16.1% of the isolates were able to produce siderophores and fix atmospheric nitrogen, using a specific primer for the genus Burkholderia (recA), the presence of at least 4 strains of Burkholderia was determined. The percentages of similarity in the recA gene varied between 98.36 to 99.51(%) with respect to the type strain. It is concluded that B. vietnamiensis would present growthpromoting characteristics due to its ability to fix nitrogen and siderophores and constitutes a potential Inoculant resource. This work has been financed by PROCIENCIA- CONCYTEC, through Contract 062- 2021-FONDECYT. [ABSTRACT FROM AUTHOR]

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