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Amaç: Bu çalışmanın amacı sıçanlarda letrozolle oluşturulmuş PKOS (polikistik over sendromu) üzerinde fisetinin antioksidan etkilerini değerlendirmektir. Gereç ve Yöntem: Çalışmada 36 adet Albino Wistar dişi sıçan kullanıldı. Dişi sıçanlara, PKOS modelini indüklemek için 21 gün boyunca %1 karboksimetil selüloz (2 mL/kg) içinde çözülmüş 1 mg/kg dozunda letrozol oral yolla uygulanmıştır. PKOS oluşturulduktan sonra 7 gün boyunca metformin ve fisetin kendi gruplarına uygulandı. Hayvanların overleri alındı ve biyokimyasal, histolojik incelemeler için uygun koşullarda saklandı. Bulgular: PKOS grubunda sağlıklı gruba göre glutatyon (GSH) seviyelerinde düşüş, malondialdehit (MDA) seviyelerinde artış görüldü. PKOS+MET+FIS25 (PKOS+20 mg/kg metformin+25 mg/kg fisetin) ve PKOS+MET+FIS50 (PKOS+20 mg/kg metformin+50 mg/kg fisetin) gruplarında doza bağlı olarak GSH seviyelerinin yükseldiği, MDA seviyelerinin düştüğü tespit edildi. Histolojik bulgularda PKOS grubunda kontrol grubuna göre çok sayıda kistik folikül içerdiği görüldü. PKOS+MET+FIS25 ve PKOS+MET+FIS50 gruplarında doza bağlı olarak kistik foliküllerin ve foliküllerin içerdiği apoptotik ve nekrotik hücrelerin azaldığı görüldü. Sonuç: Sonuç olarak, fisetin metforminin ortadan kaldıramadığı oksidatif hasarı engelleyerek faydalı etkiler göstermiştir. Fisetin antioksidan aktivite göstererek yeni hücrelerin nekroza gitmesini engellemiş ve korumuştur. Bundan dolayı metformin tedavisine fisetin eklenmesi faydalı olacaktır.

Purpose: In this study, our aim was to investigate the potential effects of strong antioxidant daidzein (DZ) on ovarian ischemia and reperfusion injury. Materials and Methods: A total of 42 female Sprague-Dawley rats were randomly divided into seven groups. For the experimental model, the clamps were removed after 3 hours of ischemia, and blood flow was provided again. Then, reperfusion process was terminated for 3 hours. Daidzein was orally administered to animals at doses of 35 and 70 mg/kg 30 minutes before ischemia (I) and ischemia and reperfusion (I/R) procedures. Results: Severe immunoreactivity of the IL-1β, IL-6 and Caspase-3 were detected in I and I/R groups. Moderate immunoreactivity of IL-1β, IL-6 and Caspase-3 was detected in I+DZ35 and I/R+DZ35 groups, and slightly positivity was detected in I+DZ70 and I/R+DZ70 groups. The SOD activity level increased in the groups treated with Daidzein, while MDA levels decreased. In addition, hemorrhage areas and inflammatory cell migration decreased in I/R+DZ70 and I/R+DZ35 groups, when compared to I/R group in a dose dependent manner. Conclusion: Daidzein has a strong protective role in the treatment of ovarian ischemia-reperfusion injury and can be used as a therapeutic agent.

Purpose: We aimed to examine the potential protective effects of phloretin and phloridzin in indomethacin induced ulcer model in mice. Materials and Methods: In total 54 female Balb/C mice were separated into nine groups. Famotidine was used as standard antiulcer agent. The phloretin and phloridzin was given at the doses of 50 and 100 mg/kg as a pre-treatment. After experimental procedures stomach tissue oxidative parameters (SOD, GSH and MDA), inflammatory cytokine TNF-α, and COX1 and COX2 mRNA expressions were analyzed. In addition, to clarify antiulcer effect mechanism of phloretin and phloridzin, numerical densities of ulcerative areas were analyzed. Results: Phloretin and phloridzin inhibited indomethacin-induced ulcer formation in dose dependent manner. Tissue inflammation and oxidative stress were increased after the indomethacin administration. Phloretin and phloridzin treatment normalized all parameters compared to indomethacin treated group. After the treatments, SOD activities and GSH levels were increased while MDA levels were decreased. Phloretin and phloridzin treatments decreased TNF-α, COX1 and COX2 mRNA expressions. Conclusion: Our results showed that phloretin and phloridzin may be an alternative treatment for peptic ulcer disease due to their potential regulatory effects against oxidative stress and inflammation.