5 results on '"Chaves, Luciana Botelho"'
Search Results
2. Development of biotinylated polyclonal anti-ribonucleoprotein IgG for detection of rabies virus antigen by direct rapid immunohistochemical test.
- Author
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Castro BS, Guedes F, Fernandes ER, Koike G, Katz ISS, Chaves LB, and Silva SR
- Subjects
- Animals, Antibodies, Monoclonal metabolism, Antibodies, Viral immunology, Antibodies, Viral metabolism, Biotinylation, Brain immunology, Brain virology, Cats, Cattle, Chiroptera, Dogs, Fluorescent Antibody Technique, Direct methods, Horses, Immunoglobulin G metabolism, Immunohistochemistry methods, Primates, Rabies diagnosis, Rabies virology, Sensitivity and Specificity, Species Specificity, Swine, Antibodies, Monoclonal immunology, Antigens, Viral immunology, Immunoglobulin G immunology, Rabies immunology, Rabies virus immunology, Ribonucleoproteins immunology
- Abstract
The direct rapid immunohistochemical test (dRIT) has been recommended for laboratorial diagnosis of rabies, especially in developing countries. The absence of commercial primary antibodies, however, still represents a major limitation to its wider use in testing. We describe here the development of a biotinylated polyclonal antibody against Rabies lyssavirus (RABV) ribonucleoprotein (RNP) and its use as a primary reagent in dRIT. Anti-RNP polyclonal horse IgG was purified by ionic exchange chromatography followed by immunoaffinity column chromatography, and its affinity, diagnostic sensitivity, and specificity were evaluated. CNS samples (120) of suspected rabies cases in different animal species were tested by dRIT, with the positive (n = 14) and negative (n = 106) results confirmed by direct fluorescence antibody testing (dFAT). Comparing the results of dRIT and dFAT, we found that the biotinylated anti-RNP IgG delivered 100% diagnostic specificity and sensibility for rabies diagnosis. Our findings show that the biotinylated anti-RNP polyclonal IgG can be produced with the quality required for application in dRIT. This work represents an important step in efforts to diagnose rabies in developing countries., (Copyright © 2020 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
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3. Delayed progression of rabies transmitted by a vampire bat.
- Author
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Katz IS, Fuoco NL, Chaves LB, Rodrigues AC, Ribeiro OG, Scheffer KC, and Asano KM
- Subjects
- Animals, Antigens, Viral, Callithrix virology, Cell Line, Tumor, Dogs virology, Mice, Rabies pathology, Rabies virology, Rabies virus classification, Chiroptera virology, Rabies veterinary, Rabies virus physiology
- Abstract
Here, we compared the growth kinetics, cell-to-cell spread, and virus internalization kinetics in N2a cells of RABV variants isolated from vampire bats (V-3), domestic dogs (V-2) and marmosets (V-M) as well as the clinical symptoms and mortality caused by these variants. The replication rate of V-3 was significantly higher than those of V-2 and V-M. However, the uptake and spread of these RABV variants into N2a cells were inversely proportional. Nevertheless, V-3 had longer incubation and evolution periods. Our results provide evidence that the clinical manifestations of infection with bat RABV variant occur at a later time when compared to what was observed with canine and marmoset rabies virus variants.
- Published
- 2016
- Full Text
- View/download PDF
4. First production of fluorescent anti-ribonucleoproteins conjugate for diagnostic of rabies in Brazil.
- Author
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Medeiros Caporale GM, Rodrigues da Silva Ade C, Peixoto ZM, Chaves LB, Carrieri ML, and Vassão RC
- Subjects
- Animals, Antibodies, Viral chemistry, Antibodies, Viral immunology, Antigens, Viral immunology, Brazil, Cell Line, Cricetinae, Fluorescein-5-isothiocyanate chemistry, Humans, Immunoglobulin G chemistry, Immunoglobulin G immunology, Male, Rabbits, Rabies immunology, Rabies virology, Rabies virus immunology, Rabies virus isolation & purification, Sensitivity and Specificity, Virus Cultivation, Fluorescent Antibody Technique, Direct methods, Rabies diagnosis, Ribonucleoproteins immunology, Viral Proteins immunology
- Abstract
The laboratory tests recommended by the World Health Organization for detection of rabies virus and evaluation of specific antibodies are performed with fluorescent antibodies against the virus, the ribonucleoproteins (RNPs), or by monoclonal antibodies. In this study, we purified the rabies virus RNPs for the production of a conjugate presenting sensibility and specificity compatible with commercial reagents. The method employed for the purification of RNPs was ultracentrifugation in cesium chloride gradient, the obtained product being used for immunizing rabbits, from which the hyperimmune sera were collected. The serum used for conjugate production was the one presenting the highest titer (1/2,560) when tested by indirect immunofluorescence. The antibodies were purified by anion exchange chromatography (QAE-Sephadex A-50),conjugated to fluorescein isothiocyanate and separated by gel filtration (Sephadex G-50). The resulting conjugate presented titers of 1/400 and 1/500 when assayed by direct immunofluorescence (DIF) and simplified fluorescence inhibition microtest, respectively. Sensibility and specificity tests were performed by DIF in 100 central nervous system samples of different animal species, presenting 100% matches when compared with the commercial reagent used as standard, independent of the conservation state of the samples. The quality reached by our conjugate will enable the standardization of this reagent for use by the laboratories performing diagnosis of rabies in Brazil, contributing to the intensification of the epidemiological vigilance and research on this disease., (Copyright 2009 Wiley-Liss, Inc.)
- Published
- 2009
- Full Text
- View/download PDF
5. EXPOSURE OF WILD BOAR (SUS SCROFA) TO THE COMMON VAMPIRE BAT AND LACK OF IMMUNE PROTECTION TO RABIES VIRUS IN BRAZILIAN HUNTERS.
- Author
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Teider-Junior, Pedro Irineu, Felipetto, Laís Giuliani, Kmetiuk, Louise Bach, Machado, Fernanda Pistori, Chaves, Luciana Botelho, Cunha Neto, Rene Dos Santos, Corona, Thaila Francini, Martins, Camila Marinelli, Bach, Renato Van Wilpe, Barros-Filho, Ivan Roque De, Dos Santos, Andrea Pires, and Biondo, Alexander Welker
- Abstract
Rabies virus is recognized as one of the most fatal zoonotic agents affecting all mammals. Wild boars (Sus scrofa), classified as a large-size exotic invasive species in Brazil with nationwide hunting permitted, may serve as an extra blood source for the common vampire bat (Desmodus rotundus). Our aim was to document wild boar exposure to vampire bats to determine the seroprevalence of rabies virus antibodies in wild boars and to determine the immune status of hunters in southern and central-western Brazilian regions. Serum samples were collected from 80 wild boars and 49 hunters from natural and degraded areas of the Atlantic Forest biome of southern Brazil and in degraded areas of the Cerrado biome of central-western Brazil. The rabies-modified rapid fluorescent focus inhibition test was performed to detect the presence of rabies virus neutralizing antibodies in wild boars and considered seropositive when ≥0.10 IU/mL. The simplified fluorescence inhibition microtest was used for samples from hunters with a titer of ≥0.50 IU/mL and considered indicative of seroconversion. While 11% (9/80) of wild boars had serum titers for rabies exposure (≥0.10 IU/mL), 88% (43/49) of corresponding hunters lacked immune protective titers (<0.50 IU/mL). Wild boars showed serum titers for rabies likely due to contact with contaminated saliva of vampire bats or from infected carcass consumption. Additionally, Brazilian wild boars can be exposed to rabies and may play an important role in the sylvatic rabies cycle by providing a blood supply for vampire bats, highlighting the possibility of direct transmission of rabies virus to hunting dogs and hunters. These findings suggested hunters are a potential risk group for contracting rabies, and the World Health Organization may consider adding this occupation to their recommendations of who should receive the pre-exposure rabies vaccination. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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