1. Evaluation of the effect of sample suspension concentration and viral load on the outcome of the rabies tissue culture infection test.
- Author
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de Souza TCP, de Sousa E Silva G, de Oliveira Fahl W, Rodrigues AC, Asano KM, Scheffer KC, Mori E, Siena A, and Iamamoto K
- Subjects
- Cattle, Animals, Viral Load, Reproducibility of Results, Brain, Rabies diagnosis, Rabies virus
- Abstract
In this study, we examined various brain suspension concentrations and viral loads in Neuro-2a cell cultures using 20 rabies-positive bovine samples. The reproducibility of results varied: 65% showed consistent outcomes across all concentrations, while 35% disagreed in at least one. Viral titers ranged from less than 25 × 10
1 to 25 × 103.50 TCID50 /mL, with 20% below 25 × 101 TCID50 /mL. Concentrations between 5% and 20% yielded over 90% agreement in positive results, but at 30%, agreement dropped from 85% to 50%. Cell confluence was successfully maintained at 5%, 10%, and 20%, while concentrations of 30% and above led to confluence loss. Low viral loads also negatively impacted reproducibility. These results suggest that sample concentration has a direct influence on preservation of cell confluence and that low viral loads may influence the reproducibility of the rabies tissue culture infection test (RTCIT)., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)- Published
- 2023
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