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Your search keyword '"Mohammad Sadeq Khosravy"' showing total 4 results
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4 results on '"Mohammad Sadeq Khosravy"'

1. Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus

Author

Vahid Asgary, Omid Kord Mafi, Mohammad Sadeq Khosravy, Alireza Janani, Nabiollah Namvar Asl, Rouzbeh Bashar, Hadi Poortaghi, Hossein Ahangari Cohan, Alireza Shoari, and Reza Ahangari Cohan

Subjects
adjuvant, silver nanoparticle, rabies vaccine, neutralizing antibodies, Medicine, Science
Abstract

Background: Nanoparticles have been considered as promising tools because of their high applicability. Recently, nanoparticles have been evaluated for their ability to increase the immune responses as adjuvants. Silver-nanoparticles (AgNPs) have shown promising results in enhancing Th-2 immune responses and to produce potent neutralizing antibodies. Neutralizing antibodies are considered as the main defense mechanism in pre- and post-exposure treatments of rabies disease. Therefore in this study, the effects of AgNPs in enhancing the immunogenicity of inactivated rabies virus were assessed. Materials and Methods: Different concentrations of AgNPs (0.2, 0.4, 0.6 and 0.8 mg/ml) were added to inactivated rabies virus. Mice were immunized by two intra-peritoneal injections of each concentration on days 1 and 7. The inactivated virus and Alum were used as negative and positive controls, respectively. Blood was collected from healthy and immunized mice, one week after the last immunization. Serum was isolated from each sample and the amounts of neutralizing antibodies were determined by Rapid Florence Focus Inhibition Test (RFFIT). The cytotoxicity of AgNPs was also assessed by in vitro MTT assay on J774A.1 cell line. Results: The results showed that 0.4, 0.6 and 0.8 mg/ml of AgNPs had significantly increased the immune responses compared to the control however, 0.2 mg/ml of AgNPs did not show a significant effect. No cytotoxicity was observed for 0.001 and 0.01 mg/ml concentrations of AgNPs but cell viability was decreased significantly at 0.1 mg/ml concentration. Conclusion: It was shown that the virus-loaded AgNPsat 0.4 mg/ml concentration could raise the neutralizing antibodies against rabies virus in mice, but their adverse effect on cell viability excludes their use as an adjuvant. Vac Res , 2014, 1 (1): 32-35

Published
2014

2. Evaluation of G2 Citric Acid-Based Dendrimer as an Adjuvant in Veterinary Rabies Vaccine

Author

Alireza Shoari, Maliheh Abedi, Vahid Asgary, Mohammad Sadeq Khosravy, Leila Ghazizadeh, Razieh Bigdeli, Majid Afshar Moayad, Reza Ahangari Cohan, Mehdi Shafiee Ardestani, Rouzbeh Bashar, Alireza Janani, and Erfan Panahnejad

Subjects
Veterinary Medicine, 0301 basic medicine, Dendrimers, Veterinary medicine, Rabies, medicine.medical_treatment, Immunology, 02 engineering and technology, Antibodies, Viral, medicine.disease_cause, Citric Acid, Cell Line, Polyethylene Glycols, Lethal Dose 50, Mice, 03 medical and health sciences, Rabies vaccine, Adjuvants, Immunologic, Neutralization Tests, In vivo, Virology, Adjuvanticity, Dendrimer, medicine, Animals, Neutralizing antibody, biology, Chemistry, Rabies virus, 021001 nanoscience & nanotechnology, Antibodies, Neutralizing, Survival Rate, Disease Models, Animal, 030104 developmental biology, Rabies Vaccines, Vaccines, Inactivated, Inactivated vaccine, biology.protein, Nanoparticles, Molecular Medicine, 0210 nano-technology, Adjuvant, medicine.drug
Abstract

For induction of an appropriate immune response, especially in the case of an inactivated vaccine, the use of an adjuvant is crucial. In this study, adjuvanticity effect of G2 dendrimer in veterinary rabies vaccine has been investigated. A nonlinear globular G2 dendrimer comprising citric acid and polyethylene glycol 600 (PEG-600) was synthesized and the toxicity was studied in vitro on the J774A.1 cell line. The adjuvanticity effect of the dendrimer was then investigated on rabies virus in NMRI mice as a model. Different concentrations of dendrimer were used to determine the best formulation for the survival of the mice after virus challenge. The rise of neutralizing antibody was also checked by rapid fluorescent focus inhibition test (RFFIT). The relative potency of the prepared formulation was finally calculated using standard NIH test and the results were compared (and discussed) with the commercially available rabies vaccine. The accuracy of dendrimer synthesis was confirmed using Fourier transform infrared (FT-IR), size, and zeta potential analysis. The in vitro toxicity assay revealed that no significant toxic effect is observed in cells when data are compared with the control group. The in vivo assay showed that a higher survival rate in the mice received a special formulation due to adjuvanticity effect of dendrimer, which is also confirmed by RFFIT. However, the relative potency of that formulation does not give expected results when compared with the alum-containing rabies vaccine. In the current investigation, the adjuvanticity effect of G2 dendrimer was demonstrated for the first time in rising of neutralizing antibodies against rabies virus. Our data confirm that nanoparticles can enhance immune responses in an appropriate manner. Moreover, engineered nanoparticles will enable us to develop novel potent multivalent adjuvants in vaccine technology.

Published
2018
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3. Development of a Time and Cost Benefit Antibody Binding Test-Based Method for Determination of Rabies Vaccine Potency

Author

Mohammad Sadeq Khosravy, Jamshid Hadjati, Alireza Janani, Vahid Asgary, Reza Ahangari Cohan, Nazanin Mojtabavi, and Tahereh Mousavi

Subjects
0301 basic medicine, Time Factors, Serial dilution, Cost-Benefit Analysis, Immunology, Modified method, Antibodies, Viral, 03 medical and health sciences, 0302 clinical medicine, Rabies vaccine, Virology, Medicine, Potency, Animals, Humans, Technology, Pharmaceutical, 030212 general & internal medicine, Vaccine Potency, Immunoassay, business.industry, Reproducibility of Results, Antigen binding, medicine.disease, 030104 developmental biology, Rabies Vaccines, Molecular Medicine, Rabies, Cost benefit, business, medicine.drug, Protein Binding
Abstract

This study is an improvement on the antibody binding test, known as ABT method, to develop a simple and fast method in comparison with NIH for determination of rabies vaccine potency. In the current study, several commercial human and veterinary vaccines were tested using both modified ABT and NIH methods. The ED50 was calculated using the probit method and the relative potency of each vaccine was measured based on the reference vaccine. The test was repeated four times to calculate the reproducibility of the method. Statistical analysis indicated that there was no significant difference between the result obtained from NIH and modified ABT method for either human or veterinary vaccines (p > 0.05). In addition, the linearity of the method (R2) was calculated as 0.94 by serial dilution of a test vaccine. Coefficient variances were determined as less than and more than 10% for the human and veterinary rabies vaccines, respectively. In conclusion, the findings suggest that the modified method could ...

Published
2017

4. Green synthesis and evaluation of silver nanoparticles as adjuvant in rabies veterinary vaccine

Author

Razieh Bigdeli, Alireza Janani, Fahimeh Baghbani-Arani, Rouzbeh Bashar, Seyed Attaollah Sadat Shandiz, Vahid Asgary, Mohammad Sadeq Khosravy, Reza Ahangari Cohan, and Alireza Shoari

Subjects
0301 basic medicine, Veterinary medicine, medicine.medical_treatment, Pharmaceutical Science, Metal Nanoparticles, 02 engineering and technology, medicine.disease_cause, Silver nanoparticle, chemistry.chemical_compound, Mice, Rabies vaccine, X-Ray Diffraction, International Journal of Nanomedicine, Drug Discovery, Alum adjuvant, Original Research, Eucalyptus, green synthesis, General Medicine, 021001 nanoscience & nanotechnology, Silver nitrate, Female, 0210 nano-technology, Adjuvant, medicine.drug, Materials science, Silver, Rabies, Biophysics, Bioengineering, Biomaterials, 03 medical and health sciences, Dogs, Adjuvants, Immunologic, adjuvant, medicine, Potency, Animals, rabies virus, Plant Extracts, Organic Chemistry, Lethal dose, Rabies virus, Green Chemistry Technology, Plant Leaves, 030104 developmental biology, chemistry, Rabies Vaccines, Microscopy, Electron, Scanning, Spectrophotometry, Ultraviolet, nanoparticles
Abstract

Vahid Asgary,1,2 Alireza Shoari,1 Fahimeh Baghbani-Arani,3 Seyed Ataollah Sadat Shandiz,4 Mohammad Sadeq Khosravy,5 Alireza Janani,1 Razieh Bigdeli,6 Rouzbeh Bashar,1 Reza Ahangari Cohan1,7 1Virology Research Group, Department of Rabies, Pasteur Institute of Iran, 2Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, 3Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, 4Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, 5Department of Laboratory of Animal Sciences, Pasteur Institute of Iran, 6Department of Genetic, Science and Research Branch, Islamic Azad University, 7New Technologies Research Group, Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran Background: Green synthesis of nanoparticles by plant extracts plays a significant role in different applications. Recently, several studies were conducted on the use of nanoparticles as adjuvant. The main aim of this study was to evaluate green synthesized silver nanoparticles (AgNPs) as adjuvant in rabies veterinary vaccine and compare the results with the existing commercially available alum adjuvant.Materials and methods: In the current study, AgNPs were prepared by the reduction of aqueous silver nitrate by leaf extract of Eucalyptus procera. The formation of AgNPs was confirmed by ultraviolet (UV)–visible spectrophotometer, scanning electron microscopy, dynamic light scattering, and X-ray diffraction analysis. Then, different amounts of AgNPs (200µg, 400µg, 600µg, and 800µg) were added to 1mL of inactivated rabies virus. The loaded vaccines (0.5mL) were injected intraperitoneally into six Naval Medical Research Institute mice in each group on days 1 and 7. On the 15th day, the mice were intracerebrally challenged with 0.03mL of challenge rabies virus (challenge virus strain-11, 20 lethal dose [20 LD50]), and after the latency period of rabies disease in mice (5days), the mice were monitored for 21days. Neutralizing antibodies against rabies virus were also investigated using the rapid fluorescent focus inhibition test method. The National Institutes of Health test was performed to determine the potency of optimum concentration of AgNPs as adjuvant. In vitro toxicity of AgNPs was assessed in L929 cell line using MTT assay. In addition, in vivo toxicity of AgNPs and AgNPs-loaded vaccine was investigated according to the European Pharmacopeia 8.0.Results: AgNPs were successfully synthesized, and the identity was confirmed by UV–visible spectrophotometry and X-ray diffraction analysis. The prepared AgNPs were spherical in shape, with an average size of 60nm and a negative zeta potential of -14mV as determined by dynamic light scattering technique. The highest percentage of viability was observed at 15mg/kg and 20mg/kg of AgNPs-loaded vaccine concentrations after injecting into the mice. The calculated potencies for alum-containing vaccine and AgNPs-loaded vaccine (dose 15mg/kg) were 1.897 and 1.303, respectively. MTT assay demonstrated that alum at the concentration of 10mg/mL was toxic, but AgNPs were not toxic. The in vivo toxicity also elucidated the safety of AgNPs and AgNPs-loaded vaccine in mice and dogs, respectively.Conclusion: In the current study, for the first time, the adjuvanticity effect of green synthesized AgNPs on veterinary rabies vaccine potency with no in vivo toxicity was elucidated according to the European Pharmacopeia 8.0. Keywords: green synthesis, nanoparticles, rabies virus, adjuvant

Published
2016

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