1. Protective properties of ginsenoside Rb3 against UV-B radiation-induced oxidative stress in HaCaT keratinocytes.
- Author
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Oh SJ, Oh Y, Ryu IW, Kim K, and Lim CJ
- Subjects
- Cell Line, Transformed, Enzyme Precursors antagonists & inhibitors, Enzyme Precursors genetics, Enzyme Precursors metabolism, Gene Expression Regulation, Glutathione agonists, Glutathione metabolism, Humans, Keratinocytes radiation effects, Matrix Metalloproteinase 2 genetics, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Oxidation-Reduction, Oxidative Stress drug effects, Reactive Oxygen Species metabolism, Signal Transduction, Skin Aging drug effects, Skin Aging radiation effects, Superoxide Dismutase genetics, Superoxide Dismutase metabolism, Ginsenosides pharmacology, Keratinocytes drug effects, Reactive Oxygen Species antagonists & inhibitors, Sunscreening Agents pharmacology, Ultraviolet Rays adverse effects
- Abstract
This work aimed to evaluate the skin anti-photoaging properties of ginsenoside Rb3 (Rb3), one of the main protopanaxdiol-type ginsenosides from ginseng, in HaCaT keratinocytes. The skin anti-photoaging activity was assessed by analyzing the levels of reactive oxygen species (ROS), pro-matrix metalloproteinase-2 (proMMP-2), pro-matrix metalloproteinase-9 (proMMP-9), total glutathione (GSH), and superoxide dismutase (SOD) activity as well as cell viability in HaCaT keratinocytes under UV-B irradiation. When HaCaT keratinocytes were exposed to Rb3 prior to UV-B irradiation, Rb3 exhibited suppressive activities on UV-B-induced ROS, proMMP-2, and proMMP-9 enhancements. On the contrary, Rb3 displayed enhancing activities on UV-B-reduced total GSH and SOD activity levels. Rb3 could not interfere with cell viabilities in UV-B-irradiated HaCaT keratinocytes. Rb3 plays a protective role against UV-B-induced oxidative stress in human HaCaT keratinocytes, proposing its potential skin anti-photoaging properties.
- Published
- 2016
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